Monolayers assembled from a glycolipid biosurfactant from Pseudozyma (Candida) antarctica serve as a high-affinity ligand system for immunoglobulin G and M

Abstract

A carbohydrate ligand system has been developed which is composed of self-assembled monolayers (SAMs) of mannosylerythritol lipid-A (MEL-A) from Pseudozyma antarctica, serving for human immunoglobulin G and M (HIgG and HIgM). The estimated binding constants from surface plasmon resonance (SPR) measurement were Ka = 9.4 x 10(6) M(-1) for HIgG and 5.4 x 10(6) M(-1) for HIgM, respectively. The binding site was not in the Fc region of immunoglobulin but in the Fab region. Large amounts of HIgG and HIgM bound to MEL-A SAMs were directly observed by atomic force microscopy.

Biosurfactants and Bioemulsifiers Biomedical and Related Applications – Present Status and Future Potentials

“…Konishi et al, (2007), Imura et al, (2007Imura et al, ( , 2008, and Ito et al, (2007), for example, developed and studied the kinetics of interactions in carbohydrate ligand systems composed of self-assembled monolayers of mannosylerythritol lipid-A (MEL-A) serving as a high-affinity, easy to handle and low-cost ligand system for immunoglobulin G and M and lectins.. Table 1 below lists the latest discoveries in the biotechnological and nanotechnological fields applicable to biosurfactants, and in particular the latest successful results of mannosylerythritol lipids (MELs) application in the enhancement of the gene transfection efficiency of cationic liposomes as well as some interesting applications of glycolipids and other biosurfactants in drug delivery and gene therapy. Biosurfactants use as a "green" alternative for high-performance nanomaterials production and, in particular, for the synthesis and stabilization of metal-bound nanoparticles will also be described.…”

Identification of Pseudozyma graminicola CBS 10092 as a Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids

“…They show not only excellent surface-active 3) and self-assembling properties [4][5][6] , but also versatile biochemical actions including antitumor and cell-differentiation activities with respect to human leukemia 7,8) , rat pheochromocytoma 9) , and mouse melanoma cells 10,11) . In addition, they show high binding affinity toward different immunoglobulins 12,13) and lectins 14) . On conventional high-level MEL producers such as P. antarctica T-34 2,15) , P. aphidis DSM 70725 16,17) and P. rugu- 18) , the product is consistently a mixture of MEL-A, -B and -C ( ); MEL-A is the major component and comprises more than 70% of all the MELs.…”

Identification of Ustilago cynodontis as a New Producer of Glycolipid Biosurfactants, Mannosylerythritol Lipids, Based on Ribosomal DNA Sequences

“…They show not only excellent surface-active 3) and self-assembling properties [4][5][6] , but also versatile biochemical actions including antitumor and cell-differentiation activities with respect to human leukemia 7,8) , rat pheochromocytoma 9) , and mouse melanoma cells 10,11) . In addition, they show high binding affinity toward different immunoglobulins 12,13) and lectins 14) . On conventional high-level MEL producers such as P. antarctica T-34 2,15) , P. aphidis DSM 70725 16,17) and P. rugulosa NBRC 10877 18) , the product is consistently a mixture of MEL-A, -B and -C ( ); MEL-A is the major component and comprises more than 70% of all the MELs.…”