Monolayer transformation by nucleophilic substitution: Applications to the creation of new monolayer assemblies

Balachander, Natarajan; Sukenik, Chaim N.

doi:10.1021/la00101a001

Cited by 327 publications

(340 citation statements)

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“…The single S2p binding energy peak present in the XPS spectrum of the oxidized thiol-modified surfaces at 169 eV suggests that the thiol groups were indeed fully oxidized to a sulfonate-like (SO 3 H) functionality. 19 However, the exact stoichiometry of the sulfur oxidation state could not be determined due to the large background O signal of the underlying SiO 2 substrate. Our results indicate that when material surfaces are exposed to dilute serum, cell attachment, spreading, and cytoskeletal organization are greater on hydrophilic surfaces relative to hydrophobic surfaces.…”

Section: Discussionmentioning

confidence: 99%

Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization

Webb

Hlady

Tresco

1998

J. Biomed. Mater. Res.

627

427

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Understanding the relationships between material surface properties, adsorbed proteins, and cellular responses is essential to designing optimal material surfaces for implantation and tissue engineering. In this study, we have prepared model surfaces with different functional groups to provide a range of surface wettability and charge. The cellular responses of attachment, spreading, and cytoskeletal organization have been studied following preadsorption of these surfaces with dilute serum, specific serum proteins, and individual components of the extracellular matrix. When preadsorbed with dilute serum, cell attachment, spreading, and cytoskeletal organization were significantly greater on hydrophilic surfaces relative to hydrophobic surfaces. Among the hydrophilic surfaces, differences in charge and wettability influenced cell attachment but not cell area, shape, or cytoskeletal organization. Moderately hydrophilic surfaces (20-40 degree water contact angle) promoted the highest levels of cell attachment. Preadsorption of the model surfaces with bovine serum albumin (BSA) resulted in a pattern of cell attachment very similar to that observed following preadsorption with dilute serum, suggesting an important role for BSA in regulating cell attachment to biomaterials exposed to complex biological media.

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Section: Discussionmentioning

confidence: 99%

Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization

Webb

Hlady

Tresco

1998

J. Biomed. Mater. Res.

627

427

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“…Proper chemical derivatization of the resulting monolayer can be achieved by using 1-bromo-16-(trichlorosilyl)hexadecane, which forms close packed ordered monolayers. 27 The bromo groups can be later substituted by azide anions which can be subsequently reduced to amine groups. Heise and co-workers 28 have also shown that mixed self-assembled monolayers (also known as SAMs) can be used for tuning the concentration of amine groups on the surface (see Fig.…”

Section: Short Survey In Chemical Modification Of Surfacesmentioning

confidence: 99%

New Developments for the Site-Specific Attachment of Protein to Surfaces

Camarero

2006

Biophys. Rev. Lett.

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Protein immobilization on surfaces is of great importance in numerous applications in biology and biophysics. The key for the success of all these applications relies on the immobilization technique employed to attach the protein to the corresponding surface. Protein immobilization can be based on covalent or noncovalent interaction of the molecule with the surface. Noncovalent interactions include hydrophobic interactions, hydrogen bonding, van der Waals forces, electrostatic forces, or physical adsorption. However, since these interactions are weak, the molecules can get denatured or dislodged, thus causing loss of signal. They also result in random attachment of the protein to the surface. Site-specific covalent attachment of proteins onto surfaces, on the other hand, leads to molecules being arranged in a definite, orderly fashion and uses spacers and linkers to help minimize steric hindrances between the protein surface. This work reviews in detail some of the methods most commonly used as well as the latest developments for the site-specific covalent attachment of protein to solid surfaces.

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“…Other interests include electrode modification [10] and catalysis [11]. A more recent application of organosilane coupling agents to develop a self-assembled systems has attracted considerable attention [12,13]. These organosilanes have hydrolyzable methoxyl groups on the silicon atom that permit covalent bond formation to a hydroxyl-substrate.…”

Section: Introductionmentioning

confidence: 99%