Monolayer-Controlled Substrate Selectivity Using Noncovalent Enzyme−Nanoparticle Conjugates

Abstract

Electrostatic interactions were used to noncovalently conjugate chymotrypsin to gold nanoparticles featuring hybrid tetraethylene(glycol)alkanethiol monolayers terminated with carboxylate groups. This conjugation process greatly alters the substrate selectivity of the adsorbed chymotrypsin, inhibiting the hydrolysis of anionic subtrates without affecting the hydrolysis rate of cationic analogues.

Inkjet-Printed Gold Nanoparticle Surfaces for the Detection of Low Molecular Weight Biomolecules by Laser Desorption/Ionization Mass Spectrometry

“…The AuNPs used in these experiments ( Figure 1) were prepared using previously published methods: TEGOH [36], TTMA [36,37], and TEGCOOH [38]. In short, the Brust-Schiffrin two-phase synthesis method was used to synthesize pentanethiol-coated AuNPs with core diameters around 2 nm [39].…”

Dynamic Light‐Scattering Analysis of the Electrostatic Interaction of Hexahistidine‐Tagged Cytochrome P450 Enzyme with Semiconductor Quantum Dots

“…One of the challenges in nano-biotechnology is to combine the intrinsic properties of biomolecules with the unusual and intriguing material properties of QDs. As covalent interactions often reduce the biological activity of the biomolecules once they are attached to the surface of the nanomaterial, [11,12] noncovalent bioconjugation techniques are preferred. [13] Nevertheless, the rich expertise in surface modification of nanomaterials and bioconjugation techniques has not always been supplemented with analytical techniques.…”

Nanoparticle Immobilization on Surfaces via Activatable Heterobifunctional Dithiocarbamate Bond Formation

“…Au-NPs [33], FePt [34], QDs [35], and ligands [36,37] were prepared according to a known procedure (see Supporting Information). The stamp material, Sylgard-184 poly(dimethylsiloxane) (PDMS), was obtained from Dow Corning.…”