Monolayer (2D) or spheroids (3D) cell cultures for nanotoxicological studies? Comparison of cytotoxicity and cell internalization of nanoparticles

Harnessing graphene oxide nanocarriers for siRNA delivery in a 3D spheroid model of lung cancer

“…This observation is supported by studies demonstrating a higher internalization of nanocarriers in HeLa (human cervical cancer cell line), MCF-7, and MDA-MB-231 (human breast cancer cell lines) cells grown as spheroids compared to 2D cultures. 7 Influence of GO flake size and functionalization on transfection efficiency in 3D spheroids…”

“…6 Therefore, cellular spheroids have become a complex heterogeneous model with cell-cell interactions and a gradient of oxygen, pH, and nanoparticle concentration between the outermost layers and the centre of mass, which is more representative of reality. 2,6,7 Moreover, cells arranged in spheroidal structures (spheroids) within 3D culture systems have been observed to exhibit a more realistic phenotype compared to cells grown in conventional 2D monolayer cultures. 8 In the last decade, gene therapy has gained increasing interest due to its high potential for the effective treatment of cancer, cardiovascular diseases, rare infections and genetic diseases.…”

Multicellular Quadruple Colorectal Cancer Spheroids as an In Vitro Tool for Antiangiogenic Potential Evaluation of Nanoparticles

“…2D cell cultures are frequently used in the literature for drug screening and evaluation of nanoparticles, but several studies show that this resource can be flawed in several points such as cell function, cell–matrix and cell–cell interaction, changes in cell shape, cells responses and do not efficiently mimic the biological tissue behavior and response to treatment. [ 52 ] The safety profile of our nanoparticles was therefore also evaluated in MCTS 3D model, as shown in Figure . All the concentrations tested were shown to be nontoxic in the first 24 h, with viability greater than 70%.…”

“…This behavior is attributed to a great absorption capacity of nanoparticles in spheroids and for more efficiently mimicking the complexity of the TME and cell-cell and cell-ECM interactions. [52,53]…”

35 Voices and 35 Years to Grow From

“…She gave an excellent presentation comparing the cellular effects of ions and nanoparticles, with an example of her research linked. 8 Additionally, I leveraged my experience in implementing bioassays and cellular and molecular biology tools. Gradually, my research focus began to center on Nanotoxicology.…”