Monoglyceride biosynthesis from coconut milk with lypase enzyme of sesame seed sprouts as biocatalyst

Abstract

Monoglycerides are oleochemical compounds that were widely used as emulsifying agents for foods, anti-bacterial and antiviruses (monolaurin, monocaprin). The objective of this research was to study the optimal reaction conditions in monoglyceride production from coconut milk. To achieve that purpose, the research was carried out with two factors, namely the ratio of coconut milk: glycerol (50:5; 50:10; 50:15 and 50:20) and esterification reaction time (0, 1, 2, 3, 4, 5 and 6 hour). The observation was conducted on free fatty acid levels of hydrolized coconut milk, esterification reaction rates, the amount of monoglyceride and fatty acid compositions in monoglyceride. The results showed that the ratio of coconut milk with glycerol of 50: 5 and the reaction time for 4 hour had the optimum esterification reaction rate. The highest of monoglyceride amount was obtained on the reaction time for 4 hours and the ratio of coconut milk with glycerol of 50:5.

Transesterification synthesis of high-yield biodiesel from black soldier fly larvae by using the combination of Lipase Eversa Transform 2.0 and Lipase SMG1

“…Increasing the molar ratio will make the reaction move forward and accelerate the reaction process. However, the short-chain alcohol has a toxic effect on the enzyme activity (Xu et al, 2012), resulting in a notable reduction on the final product content (Su'i et al, 2021). Therefore, the optimal condition for FAME production was established to be a molar ratio of BSFL lipids to methanol at 1:3, reaction time at 25 °C and a mixture of Lipase Eversa Transform 2.0 and Lipase SMG1 as catalysis.…”

Enzymatic Glycerolysis of Palm Kernel Olein-Stearin Blend for Monolaurin Synthesis as an Emulsifier and Antibacterial

“…Zha et al found that the optimum conditions for coconut oil glycerolysis at 50 °C in the microemulsion system were the coconut oil to glycerol molar ratio 1:4, the concentration of Novozym 435 and sodium (bis-2-ethyl-hexyl) sulfosuccinate 8 and 16%, respectively [ 10 ]. The separation of monoacylglycerol or monolaurin can be achieved using column chromatography with silica gel as the stationary phase and a mixture of solvents n -hexane and ethyl acetate, which are gradually eluted [ 11 , 12 ], or the solvent method using n -hexane and hydroalcoholic solution [ 13 ]. However, there is limited information on the various factors that can impact the effectiveness of PKOo-PKS blend glycerolysis to produce monolaurin, including the concentration of enzyme, the molar ratio of oil to glycerol, the solvent to oil ratio, and the temperature reaction.…”

One-step method for improving the stability of coconut milk emulsion and keeping its flavor based on dynamic high-pressure microfluidization

“…Coconut milk is rich in protein, fat, minerals, vitamins, etc. The fat content of coconut milk can reach 35-37%, and protein can reach 2-5% (Su'i et al, 2020;Lad & Murthy, 2012). Because it is rich nutrition and it has a unique flavor, it is often used in cooking, especially in Thailand and Southeast Asian cuisine (Schiassi et al, 2020;Tansakul & Chaisawang, 2006).…”