Monoglucosyl-rutin as a potential radioprotector in mammalian cells

Sunada, Shigeaki; Fujisawa, Hiroshi; Cartwright, Ian M.; Maeda, Jun; Brents, Colleen A.; Mizuno, Kazue; Aizawa, Yasushi; Kato, Takamitsu A.; Uesaka, Mitsuru

doi:10.3892/mmr.2014.2181

Cited by 31 publications

(29 citation statements)

References 12 publications

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“…It has been shown that rutin prevents UV-induced changes in skin cell membrane function and, by inhibiting the activity of cyclooxygenases and lipoxygenases as well as non-enzymatic lipid mediators generation, inhibits pro-inflammatory signaling [20,21]. Moreover, rutin exerts cytoprotective effects on cells exposed to various types of physical factors by substantially increasing their viability [22,23]. Additionally, rutin exerts cytoprotective effects on cells exposed to different types of radiation by substantially increasing their viability [16].…”

Section: Introductionmentioning

confidence: 99%

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Gęgotek

Jarocka-Karpowicz

2020

Nutrients

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The combination of ascorbic acid and rutin, commonly used in oral preparations for their antioxidant and anti-inflammatory properties, can also be used to protect skin cells from the effects of UV radiation in sunlight. Here, we tested the potential protective effect of ascorbic acid and rutin used together in UVB-irradiated human skin fibroblasts, and assessed the proteomic profile of these cells, grown in a three-dimensional (3D) system. Proteomic findings revealed a combined effect of ascorbic acid and rutin in UV-irradiated fibroblasts against overexpression of pro-inflammatory signaling proteins and DNA reorganization/expression. These effects were not observed when cells were treated with either compounds alone. The antioxidant effects of ascorbic acid and rutin also prevented protein modifications by lipid peroxidation products. Further, ascorbic acid stimulated rutin-protein adduct formation, which supports intra/extracellular signaling and the Nrf2/ARE antioxidant pathway, contributing to the protective effects against UV-induced oxidative stress. The combined effect of ascorbic acid and rutin suggests that this combination of compounds is potentially effective against skin damage caused by UV radiation.

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Section: Introductionmentioning

confidence: 99%

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Gęgotek

Jarocka-Karpowicz

2020

Nutrients

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“…Rutin is a flavonoid found in plant extracts, and inhibits the activities of cyclooxygenases and lipoxygenases, thereby reducing pro-inflammatory processes [8]. Moreover, rutin exerts cytoprotective effects on cells exposed to various types of radiation by substantially increasing their viability [79]. So far, the interaction of orally administered rutin with ascorbic acid has been demonstrated in terms of their antiinflammatory and vascular sealing actions [4,51].…”

Section: Introductionmentioning

confidence: 99%

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

et al. 2019

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The combination of ascorbic acid and rutin is frequently used in oral preparations. However, despite numerous protective effects of each component individually, their combined effect on ultraviolet (UV)-irradiated skin cells has never been evaluated. The aim of this study was to evaluate the combined effect of ascorbic acid and rutin on human keratinocytes and fibroblasts exposed to UVA and UVB radiation. Skin keratinocytes and fibroblasts exposed to UVA and UVB radiation were treated with ascorbic acid or/and rutin. The total antioxidant properties of both components, as well as their effect on cellular pro-and antioxidant status, lipid and protein oxidation, transmembrane transport, and pro-inflammatory and pro/ antiapoptotic protein expression were measured. The combination of ascorbic acid and rutin had higher antioxidant properties compared to the activity of the single compound alone, and showed a stronger effect against UV-induced reactive oxygen species generation. The ascorbic acid and rutin combination also showed increased antioxidant enzyme activity (catalase, superoxide dismutase, thioredoxin reductase), which was impaired following UV irradiation. Moreover, ascorbic acid additional stimulated UV-induced bilitranslocase activity responsible for rutin transport, and therefore favored rutin effect on Nrf2 pathway, simultaneously differentiating the reaction of keratinocytes and fibroblasts. In keratinocytes, Nrf2 is strongly activated, while in fibroblasts decreased Nrf2 activity was observed. Used mixture, also significantly silenced UV-induced expression of pro-inflammatory factor NFκB and pro-apoptotic proteins such as caspases 3, 8, and 9. These results showed that ascorbic acid and rutin are complementary in their antioxidant actions, transport and signaling functions. Their combined antioxidant, antiinflammatory and antiapoptotic actions suggest rutin and ascorbic acid are a potentially cytoprotective team against UV-induced skin damage.

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“…The CHO10B2 (wild-type), V3 (protein kinase DNA-activated catalytic polypeptide deficient) (12) (16). Heavy ion irradiation was performed at the National Institute of Radiological Sciences (Chiba, Japan).…”

Section: Methodsmentioning

confidence: 99%

Investigation of the relative biological effectiveness and uniform isobiological killing effects of irradiation with a clinical carbon SOBP beam on DNA repair deficient CHO cells

Sunada¹,

Cartwright²,

Hirakawa³

et al. 2017

Oncology Letters

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Abstract. Spread-out Bragg peak (SOBP) C ions have been used clinically in charged particle radiation therapy for years. An SOBP beam consists of various monoenergetic Bragg peaks; however, the biological effect of irradiation with an SOBP beam track has not been well-studied. In order to determine the clinically prospective molecular targets, radiosensitivity to the beam track in DNA repair deficient cell lines was investigated. A total of four distinct Chinese hamster ovary (CHO) cell lines, including CHO10B2 (wild-type), V3 (protein kinase DNA-activated catalytic polypeptide deficient), 51D1 (RAD51 paralog D deficient) and PADR9 [poly(ADP-ribose) polymerase (PARP) deficient], were irradiated with gamma-rays, C ions (290 MeV/n) and Fe ions (500 MeV/n), in order to compare cellular lethality. An OptiCell™ culture system was used to evaluate the lethality at distinct depths of SOBP C ions. Relative biological effectiveness (RBE) values of C ions (linear energy transfer (LET), 13 and 70 keV/µm) and Fe ions (LET, 200 keV/µm) were calculated from cell survival using colony formation assay with standard cell dishes. All cell lines exhibited similar sensitivity to 70 keV/µm C ions and 200 keV/ µm Fe ions. Furthermore, V3 cells did not exhibit increased sensitivity to high LET C ions and Fe ions, compared with low LET gamma-rays and C ions, and 51D1 cells irradiated with 13 keV/µm C ions exhibited relatively high RBE values among the tested cell lines. Conversely, PADR9 cells exhibited low RBE values for 13 keV/µm C ions and high RBE values for 70 keV/µm C ions. Obtained using the OptiCell system, the survival fractions in the SOBP region were uniform for wild-type and PADR9 cells. Conversely, V3 and 51D1 cells exhibited decreased cell death in the distal region of the SOBP. These results indicated that PARP is a more effective target for clinical beam therapy, compared with the non-homologous end joining repair and homologous recombination repair pathways. PARP deficiency may be an optimal target for C ion therapy and the results of the present study may contribute to the development of a more effective heavy ion radiation therapy. IntroductionHeavy ion radiation therapy began in the 1970s in the USA as a more efficient radiation therapy compared with classical X-ray therapy (1,2). Heavy ion therapy is gaining prevalence, and has been increasingly utilized in Japan, China, Germany and Italy (1,2). Heavy ions exhibit three advantageous cancer cell killing characteristics when compared with X-rays. Heavy ions exhibit a more efficient dose distribution in cancer tissues due to the Bragg peak. The phenomenon allows for the majority of the dose to be deposited within the cancer tissues, avoiding unnecessary radiation exposure to normal tissues. In addition, heavy ions form complex DNA lesions within the cell due to the large amount of energy deposited during particle/DNA interactions. Heavy ions are able to produce more complex DNA lesions compared with X-rays and gamma-rays (1,2). Complex DNA damage lesions are more diff...

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Monoglucosyl-rutin as a potential radioprotector in mammalian cells

Cited by 31 publications

References 12 publications

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Cytoprotective Effect of Ascorbic Acid and Rutin against Oxidative Changes in the Proteome of Skin Fibroblasts Cultured in a Three-Dimensional System

Rutin and ascorbic acid cooperation in antioxidant and antiapoptotic effect on human skin keratinocytes and fibroblasts exposed to UVA and UVB radiation

Investigation of the relative biological effectiveness and uniform isobiological killing effects of irradiation with a clinical carbon SOBP beam on DNA repair deficient CHO cells

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