Monogenean Parasite Cultures: Current Techniques and Recent Advances

Hutson, Kate S.; Brazenor, Alexander K.; Vaughan, David B.; Trujillo‐González, Alejandro

doi:10.1016/bs.apar.2018.01.002

Cited by 37 publications

(25 citation statements)

References 107 publications

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“…The continued pursuit to optimise aquatic parasite culture techniques will enable researchers In vitro and in vivo monogenean cultures have been used to examine the efficacy of commercial [91] and natural products [17,92,93] as well as biocontrols [94]. In vivo culture enabled parasite life cycle parameters to be examined in response to varied environmental parameters which were used to develop strategic treatment regimens on aquaculture farms [10,74].…”

Section: Discussionmentioning

confidence: 99%

“…The current impetus to culture parasites is driven by the need for fundamental and applied research to support human and animal health agendas. Successful, continuous aquatic parasite cultures can be maintained for years or even decades [6][7][8][9][10][11], yet short term cultures may also be valuable for targeted experiments or the production of parasite tissues free of host contaminants. Indeed, many successful helminth culture media were developed from short-term maintenance media used in biochemical experiments.…”

Section: Origins Establishment Maintenance and Ethicsmentioning

confidence: 99%

“…Establishing a new culture may involve co-habitation of laboratory animals with wild or infected farmed stock, although individual exposure to a known quantity of infective stages is preferred in the interest of animal welfare and to avoid over-dispersion, which can also compromise hosts [10]. Where possible, recipient laboratory hosts should have no previous history of infection.…”

Section: In Vivo Culturesmentioning

confidence: 99%

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Aquatic Parasite Cultures and Their Applications

Hutson

Cable

Grutter

et al. 2018

Trends in Parasitology

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In this era of unprecedented growth in aquaculture and trade, aquatic parasite cultures are essential to better understand emerging diseases and their implications for human and animal health. Yet culturing parasites presents multiple challenges, arising from their complex, often multi-host life cycles, multiple developmental stages, variable generation times and reproductive modes. Furthermore, the essential environmental requirements of most parasites remain enigmatic. Despite these inherent difficulties, in vivo and in vitro cultures are being developed for a small but growing number of aquatic pathogens. Expanding this resource will facilitate diagnostic capabilities and treatment trials, thus supporting the growth of sustainable aquatic commodities and communities. Aquatic parasite cultures permit advances in human, animal and environmental health Parasite cultures (see Glossary) facilitate the completion of life cycles over successive generations, either in vivo (i.e. on or in a host) or in vitro (i.e. in the absence of the host). Alternatively, if the full life cycle cannot be completed, some parasite development or extension of life span might be achieved. Ideally, cultures should have defined origins and depending on the application, it may be desirable for them to be genetically restricted and maintain the same restricted subset of genotypes across generations. The ultimate goal of a parasite culture is to provide readily manipulated material of defined life-stages for replicable experimental research. Aquatic cultures include the use of molluscan, crustacean, fish, amphibian, avian and mammalian hosts for a wide diversity of parasites (Table 1). Recent rapid growth in the mass production of aquatic animals for food, alongside growth in international trade, rapid domestication and application of new technologies, drive

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Section: Discussionmentioning

confidence: 99%

Section: Origins Establishment Maintenance and Ethicsmentioning

confidence: 99%

Section: In Vivo Culturesmentioning

confidence: 99%

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Aquatic Parasite Cultures and Their Applications

Hutson

Cable

Grutter

et al. 2018

Trends in Parasitology

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“…shrimp. The commercially important monogenean N. girellae is continuously cultured in the separate MPL culture facility at JCU (see Hutson et al 2018). Prior to experimentation, freshly laid N. girellae eggs were isolated from the culture and incubated at 24°C in a large glass Petri dish containing fresh, filtered sea water (salinity = 35 ppt).…”

Section: Animals and Experimental Designmentioning

confidence: 99%

“…Prior to experimentation, freshly laid N. girellae eggs were isolated from the culture and incubated at 24°C in a large glass Petri dish containing fresh, filtered sea water (salinity = 35 ppt). Eggs were monitored daily under a Leica M60 dissection microscope for embryonic development and hatching (see Hutson et al 2018). Free-swimming larvae (oncomiracidia) hatched on Day 4 (see Brazenor & Hutson 2015) and were collected via pipette and counted before immediately being transferred to a glass beaker of fresh, filtered seawater for the experiment.…”

Section: Animals and Experimental Designmentioning

confidence: 99%

Cleaner shrimp remove parasite eggs on fish cages

Vaughan¹,

Grutter²,

Hutson³

2018

Aquacult. Environ. Interact.

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Benthic stages of cultured fishes' ectoparasites are a major contributor to persistent reinfections in aquaculture. These stages are resistant to chemical therapies and are costly to manage in terms of time and labour. Cleaner shrimp, unlike cleaner fishes, prey on benthic stages, suggesting they have the potential to reduce parasite reinfection pressure without having to be in direct contact with the client fish. Cleaner shrimp have never been used as biocontrols in commercial aquaculture, but offer an advantage over cleaner fishes in that they are not susceptible to the ectoparasites of their clients. We present the first investigation of a cultured cleaner shrimp, Lysmata vittata, as a biocontrol agent against the eggs of the economically important cosmopolitan ectoparasite Neobenedenia girellae infecting cultured juvenile grouper, Epinephelus lanceolatus, under simulated recirculating aquaculture conditions. L. vittata removed the eggs of N. girellae entangled on the mesh of the culture cages and significantly reduced N. girellae recruitment to fish by ~87%. Our results demonstrate the value of cleaner shrimp in addressing ectoparasite problems and highlight the importance of investigating novel biocontrol strategies in aquaculture.

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Teleost Fish

Urdeș¹,

Hangan²

2023

Pathology and Epidemiology of Aquatic Animal Diseases for Practitioners

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Monogenean Parasite Cultures: Current Techniques and Recent Advances

Cited by 37 publications

References 107 publications

Aquatic Parasite Cultures and Their Applications

Aquatic Parasite Cultures and Their Applications

Cleaner shrimp remove parasite eggs on fish cages

Teleost Fish

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