Monocytes control natural killer cell differentiation to effector phenotypes

Soderquest, Katrina; Powell, Nick; Luci, Carmelo; Rooijen, Nico van; Hidalgo, Andrés; Geissmann, Frédéric; Walzer, Thierry; Lord, Graham M.; Martín-Fontecha, Alfonso

doi:10.1182/blood-2010-10-312264

Cited by 76 publications

(85 citation statements)

References 46 publications

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“…CNS-resident NK cells mediate immune suppression during the acute stage of CNS autoimmune diseases via cross-talk with microglia/macrophages of the CNS (17,18). The reciprocal interaction between NK cells and monocytes/macrophages of the innate immune system has also been considered crucial for the immune activity of these cells in various inflammatory conditions (19)(20)(21)(22). However, a more profound understanding of the detrimental and beneficial roles of macrophages in EAE has been reported, which relies on the new recognition of monocyte-derived macrophages being functionally distinct from microglia-derived macrophages (23)(24)(25)(26)(27).…”

Section: Ly6cmentioning

confidence: 99%

Acetylcholine-producing NK cells attenuate CNS inflammation via modulation of infiltrating monocytes/macrophages

Jiang

Han

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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The nonneural cholinergic system of immune cells is pivotal for the maintenance of immunological homeostasis. Here we demonstrate the expression of choline acetyltransferase (ChAT) and cholinergic enzymes in murine natural killer (NK) cells. Ly6Chi monocytes directly via the disruption of tolerance and inhibited the production of proinflammatory cytokines. Interestingly, ChAT + NK cells and CCR2 + Ly6C hi monocytes formed immune synapses; moreover, the impact of ChAT + NK cells was mediated by α7-nicotinic acetylcholine receptors. Finally, the NK cell cholinergic system up-regulated in response to autoimmune activation in multiple sclerosis, perhaps reflecting the severity of disease. Therefore, this study extends our understanding of the nonneural cholinergic system and the protective immune effect of acetylcholine-producing NK cells in autoimmune diseases.

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Section: Ly6cmentioning

confidence: 99%

Acetylcholine-producing NK cells attenuate CNS inflammation via modulation of infiltrating monocytes/macrophages

Jiang

Han

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…The T-box family member Eomesodermin (Eomes) is essential for the early transition from CD11b − to the DP stage (Gordon et al, 2012). Another T-box family member, T-bet, drives terminal NK cell maturation by reducing proliferation (Townsend et al, 2004), up-regulating the expression of S1pr5 mRNA (Jenne et al, 2009), and driving the transition to the CD27 − mature stage (Soderquest et al, 2011). Here, in an effort to identify novel TFs involved in NK cell maturation, we screened microarray data for genes up-regulated in mature NK cells and selected Zeb2 (zinc finger E-boxbinding protein 2) as a putative regulator of maturation.…”

Section: Terminal Nk Cell Maturation Is Controlled By Concerted Actiomentioning

confidence: 99%

“…3 D). Next, we isolated lung mononuclear cells at different time points after B16 injection and followed NK cell degranulation and −/− mice lack mature CD27 − NK cells (Soderquest et al, 2011), their peripheral NK cells display high rates of apoptosis (Townsend et al, 2004), and they have an impaired capacity to exit BM as a result of decreased expression of S1PR5 (Jenne et al, 2009). These observations prompted us to compare the phenotype of NK cells mutant for either TF in more details.…”

Section: Not Depicted) Correlating With This Zeb2mentioning

confidence: 99%

Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection

Helden¹,

Goossens²,

Daussy³

et al. 2015

J Cell Biol

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“…1B). In the intermediary phase of our culture system, these monocytic cells might be important for promoting NK cell differentiation in terms of providing secreted factors and/or cellular interactions such as transpresenting IL-15 via IL-15 receptor expressed on monocytes [32][33][34] To further characterize our NK cell differentiation system, we performed microarray profiling at different stages of differentiation. The obtained data demonstrate that a broad range of genes characteristic for NK cells was reproducibly upregulated.…”

Section: Lehmann Et Almentioning

confidence: 99%

Ex Vivo Generated Natural Killer Cells Acquire Typical Natural Killer Receptors and Display a Cytotoxic Gene Expression Profile Similar to Peripheral Blood Natural Killer Cells

Lehmann

Spanholtz

Osl

et al. 2012

Stem Cells and Development

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Ex vivo differentiation systems of natural killer (NK) cells from CD34+ hematopoietic stem cells are of potential importance for adjuvant immunotherapy of cancer. Here, we analyzed ex vivo differentiation of NK cells from cord blood-derived CD34+ stem cells by gene expression profiling, real-time RT-PCR, flow cytometry, and functional analysis. Additionally, we compared the identified characteristics to peripheral blood (PB) CD56 bright and CD56dim NK cells. The data show sequential expression of CD56 and the CD94 and NKG2 receptor chains during ex vivo NK cell development, resulting finally in the expression of a range of genes with partial characteristics of CD56 bright and CD56 dim NK cells from PB. Expression of characteristic NK cell receptors and cytotoxic genes was mainly found within the predominant ex vivo generated population of NKG2A + NK cells, indicating the importance of NKG2A expression during NK cell differentiation and maturation. Furthermore, despite distinct phenotypic characteristics, the detailed analysis of cytolytic genes expressed within the ex vivo differentiated NK cells revealed a pattern close to CD56 dim NK cells. In line with this finding, ex vivo generated NK cells displayed potent cytotoxicity. This supports that the ex vivo differentiation system faithfully reproduces major steps of the differentiation of NK cells from their progenitors, constitutes an excellent model to study NK cell differentiation, and is valuable to generate large-scale NK cells appropriate for immunotherapy.

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Monocytes control natural killer cell differentiation to effector phenotypes

Cited by 76 publications

References 46 publications

Acetylcholine-producing NK cells attenuate CNS inflammation via modulation of infiltrating monocytes/macrophages

Acetylcholine-producing NK cells attenuate CNS inflammation via modulation of infiltrating monocytes/macrophages

Terminal NK cell maturation is controlled by concerted actions of T-bet and Zeb2 and is essential for melanoma rejection

Ex Vivo Generated Natural Killer Cells Acquire Typical Natural Killer Receptors and Display a Cytotoxic Gene Expression Profile Similar to Peripheral Blood Natural Killer Cells

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