Monocytes are required for optimum in vitro stimulation of bovine peripheral blood mononuclear cells by non-methylated CpG motifs

Pontarollo, Reno; Rankin, Robert; Babiuk, Lorne A.; Godson, Dale L.; Griebel, Philip; Hecker, Rolf; Krieg, Arthur Μ.; Hurk, Sylvia van Drunen Littel‐van den

doi:10.1016/s0165-2427(01)00379-8

Cited by 66 publications

(29 citation statements)

References 37 publications

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“…and combining it with the CpG oligodeoxynucleotide ODN 2007 (21). Emulsigen was added to a final concentration of 50% (vol/vol).…”

Section: Methodsmentioning

confidence: 99%

“…Vaccination with culture filtrate proteins (CFP) from Mycobacterium tuberculosis and M. bovis has been shown to induce protection against tuberculosis in mouse models (3,4,23). The vaccination of cattle with M. bovis CFP formulated with Emul-sigen, an emulsified oil-in-water adjuvant, and combined with a specific CpG oligodeoxynucleotide (ODN) (21) has been shown to induce a significant level of protection against tuberculosis (33). The aims of the present study were to determine if the protection could be enhanced further by increasing the concentration of adjuvant in the vaccine as well as by revaccinating animals twice instead of once.…”

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confidence: 99%

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Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine andMycobacterium bovisBCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone

et al. 2005

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The development of a subunit protein vaccine for bovine tuberculosis which could be used either in combination with Mycobacterium bovis BCG (to improve the efficacy of that vaccine) or alone would offer significant advantages over currently available strategies. A study was conducted with cattle to determine the protective efficacy of a strategy based on concurrent immunization with an M. bovis culture filtrate (

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“…and combining it with the CpG oligodeoxynucleotide ODN 2007 (21). Emulsigen was added to a final concentration of 50% (vol/vol).…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine andMycobacterium bovisBCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone

et al. 2005

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“…Whether bovine B cells respond directly to CpG ODN is still somewhat controversial. Evidence presented by Pontarollo et al (2002) indicates that myeloid cells are required for optimal B cell proliferation in response to CpG ODN. We have further elucidated that CD14 + monocytes are the myeloid cell population required for a robust, B cell proliferative response.…”

Section: Tlr-induced Innate Immune Responses In B Cellsmentioning

confidence: 99%

Modulation of B cell responses by Toll-like receptors

et al. 2010

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B lymphocytes are well known because of their key role in mediating humoral immune responses. Upon encounter with antigen and on cognate interaction with T cells, they differentiate into antibody-secreting plasma cells, which are critical for protection against a variety of pathogens. In addition to their antibody-production function, B cells are efficient antigen-presenting cells and express a variety of pathogen recognition receptors (PRRs). Engagement of these PRRs with their respective ligands results in cytokine and chemokine secretion and the upregulation of co-stimulatory molecules. These events constitute innate immune responses. Toll-like receptor (TLR) activation provides a third signal for B cell activation and is essential for optimal antigen-specific antibody responses. In some situations, TLR activation in B cells can result in autoimmunity. The purpose of this review is to provide some insights into the way that TLRs influence innate and adaptive B cell responses.

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“…The CpG ODN we used was ODN 2007 (TCGTCGTTGTCGTTTTGTCGTT; CpG motifs are underlined). We have shown that this ODN stimulates bovine peripheral blood mononuclear cells (PBMC) in vitro (33,36). To determine whether immune responses were induced by the CpG dinucleotides, we also used a non-CpG ODN, 2041 (CTGG TCTTTCTGGTTTTTTTCTGG).…”

Section: Cells and Virusmentioning

confidence: 99%

The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

Ioannou¹,

Griebel²,

Hecker

et al. 2002

J Virol

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117

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The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species.

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Monocytes are required for optimum in vitro stimulation of bovine peripheral blood mononuclear cells by non-methylated CpG motifs

Cited by 66 publications

References 37 publications

Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine andMycobacterium bovisBCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone

Vaccination of Cattle with a CpG Oligodeoxynucleotide-Formulated Mycobacterial Protein Vaccine andMycobacterium bovisBCG Induces Levels of Protection against Bovine Tuberculosis Superior to Those Induced by Vaccination with BCG Alone

Modulation of B cell responses by Toll-like receptors

The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

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