Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels

Patel, Vyoma; Williams, Helen; Li, Stephen C.H.; Fletcher, J.; Medbury, Heather

doi:10.3389/fimmu.2021.616305

Cited by 3 publications

(17 citation statements)

References 56 publications

(69 reference statements)

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“…Of note, the skewing of monocytes to an atherogenic state in generally healthy individuals with a perturbed lipid profile was evident for all three monocyte subsets [23,42]. Though there were still notable differences between the subsets, these were overshadowed by differences between individuals, such that in some individuals, all three monocyte subsets produced inflammatory cytokines or expressed recruitment markers at a higher level than in other individuals.…”

Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 93%

“…The phenotype that monocytes acquire in generally healthy individuals, dyslipidemia or CVD (Tables 2, 4 and 5) is lipid-specific with, for example, TLR2 increased relative to total and LDL cholesterol [42], while most of the adhesion markers examined displayed an inverse relationship with ApoA1 [23]. The inverse correlation of the recruitment markers examined with ApoA1 [23] suggests that monocytes have an increased potential to extravasate when ApoA1 is low.…”

Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 97%

“…Additional monocyte surface markers have also been proposed to allow better separation of the intermediate and nonclassical subsets, such as their respective positive and negative expression of the chemokine receptor CCR2 [20]. Recently, it has been proposed that more than three subsets exist [21,22], which is unsurprising considering monocytes exist on a continuum (of expression of CD14 and CD16: as seen in Figure 1) rather than in discrete populations and that even within each subset, the expression of markers (such as adhesion molecules) is heterogeneous [23]. While classification into three subsets may be simplistic, it has been found to be relevant in studies of monocyte associations with CVD.…”

Section: Monocyte Subset Proportions In Cvdmentioning

confidence: 99%

“…Intermediate monocytes (compared to the other subsets) have been implicated as major producers of inflammatory cytokines, including Interleukin (IL)-1β, IL-6 and Tumor necrosis factor (TNF)α in response to lipopolysaccharide (LPS: an outer membrane component of bacteria) [42]. Similarly, the monocyte subsets have been shown to have different potential for adhesion and migration [23,43], therefore proportional changes may further impact atherogenesis through altered monocyte entry to the plaque.…”

Section: Monocyte Functional Changes In Cvdmentioning

confidence: 99%

“…Firstly, monocytes from hyperlipidemic patients display increased cytokine production (post LPS stimulation) [61] and adhesion to endothelial cells [61,62] compared with controls. Secondly, even for generally healthy controls, monocytes are skewed towards an atherogenic state in individuals with a perturbed lipid profile [23,37,42]. Thirdly, high fat feeding increases monocyte adhesion and chemotaxis [63,64].…”

Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 99%

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Nature versus Number: Monocytes in Cardiovascular Disease

Williams

Mack

et al. 2021

IJMS

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Monocytes play a key role in cardiovascular disease (CVD) as their influx into the vessel wall is necessary for the development of an atherosclerotic plaque. Monocytes are, however, heterogeneous differentiating from classical monocytes through the intermediate subset to the nonclassical subset. While it is recognized that the percentage of intermediate and nonclassical monocytes are higher in individuals with CVD, accompanying changes in inflammatory markers suggest a functional impact on disease development that goes beyond the increased proportion of these ‘inflammatory’ monocyte subsets. Furthermore, emerging evidence indicates that changes in monocyte proportion and function arise in dyslipidemia, with lipid lowering medication having some effect on reversing these changes. This review explores the nature and number of monocyte subsets in CVD addressing what they are, when they arise, the effect of lipid lowering treatment, and the possible implications for plaque development. Understanding these associations will deepen our understanding of the clinical significance of monocytes in CVD.

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Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 93%

Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 97%

Section: Monocyte Subset Proportions In Cvdmentioning

confidence: 99%

Section: Monocyte Functional Changes In Cvdmentioning

confidence: 99%

Section: Monocyte Functional Changes Associated With Lipid Levelsmentioning

confidence: 99%

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Nature versus Number: Monocytes in Cardiovascular Disease

Williams

Mack

et al. 2021

IJMS

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Influence of bariatric surgery on the peripheral blood immune system of female patients with morbid obesity revealed by high-dimensional mass cytometry

et al. 2023

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IntroductionObesity is associated with low-grade chronic inflammation, altered levels of adipocytokines, and impaired regulation of gastrointestinal hormones. Secreted, these factors exert immunostimulatory functions directly influencing peripheral immune cells.MethodsIn the realm of this study, we aimed to investigate the composition and activation status of peripheral blood immune cells in female patients with morbid obesity compared to lean controls using high-dimensional mass cytometry. Besides, we also assessed the influence of bariatric surgery with respect to its ability to reverse obesity-associated alterations within the first-year post-surgery.ResultsPatients with morbid obesity showed typical signs of chronic inflammation characterized by increased levels of CRP and fibrinogen. Apart from that, metabolic alterations were characterized by increased levels of leptin and resistin as well as decreased levels of adiponectin and ghrelin compared to the healthy control population. All these however, except for ghrelin levels, rapidly normalized after surgery with regard to control levels. Furthermore, we found an increased population of monocytic CD14+, HLA-DR-, CD11b+, CXCR3+ cells in patients with morbid obesity and an overall reduction of the HLA-DR monocytic expression compared to the control population. Although CD14+, HLA-DR-, CD11b+, CXCR3+ decreased after surgery, HLA-DR expression did not recover within 9 – 11 months post-surgery. Moreover, compared to the control population, patients with morbid obesity showed a perturbed CD4+ T cell compartment, characterized by a strongly elevated CD127+ memory T cell subset and decreased naïve T cells, which was not recovered within 9 – 11 months post-surgery. Although NK cells showed an activated phenotype, they were numerically lower in patients with morbid obesity when compared to healthy controls. The NK cell population further decreased after surgery and did not recover quantitatively within the study period.ConclusionsOur results clearly demonstrate that the rapid adaptions in inflammatory parameters and adipocytokine levels that occur within the first year post-surgery do not translate to the peripheral immune cells. Apart from that, we described highly affected, distinct immune cell subsets, defined as CD127+ memory T cells and monocytic CD14+, HLA-DR, CD11b+, CXCR3+ cells, that might play a significant role in understanding and further decoding the etiopathogenesis of morbid obesity.

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Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences

Williams,

Mack,

Baraz

et al. 2023

IJMS

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The three subsets of human monocytes, classical, intermediate, and nonclassical, show phenotypic heterogeneity, particularly in their expression of CD14 and CD16. This has enabled researchers to delve into the functions of each subset in the steady state as well as in disease. Studies have revealed that monocyte heterogeneity is multi-dimensional. In addition, that their phenotype and function differ between subsets is well established. However, it is becoming evident that heterogeneity also exists within each subset, between health and disease (current or past) states, and even between individuals. This realisation casts long shadows, impacting how we identify and classify the subsets, the functions we assign to them, and how they are examined for alterations in disease. Perhaps the most fascinating is evidence that, even in relative health, interindividual differences in monocyte subsets exist. It is proposed that the individual’s microenvironment could cause long-lasting or irreversible changes to monocyte precursors that echo to monocytes and through to their derived macrophages. Here, we will discuss the types of heterogeneity recognised in monocytes, the implications of these for monocyte research, and most importantly, the relevance of this heterogeneity for health and disease.

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Monocyte Subset Recruitment Marker Profile Is Inversely Associated With Blood ApoA1 Levels

Cited by 3 publications

References 56 publications

Nature versus Number: Monocytes in Cardiovascular Disease

Nature versus Number: Monocytes in Cardiovascular Disease

Influence of bariatric surgery on the peripheral blood immune system of female patients with morbid obesity revealed by high-dimensional mass cytometry

Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences

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