MONOCULM 3, an Ortholog of WUSCHEL in Rice, Is Required for Tiller Bud Formation

Lu, Zefu; Shao, Gaoneng; Xiong, Jinsong; Jiao, Yongqing; Wang, Jing; Liu, Guifu; Meng, Xiangbing; Liang, Yan; Xiong, Guosheng; Wang, Yonghong; Li, Jiayang

doi:10.1016/j.jgg.2014.12.005

Cited by 115 publications

(102 citation statements)

References 39 publications

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“…Nevertheless, it is possible that the general effect of WUS on SAM function has an indirect role on AM initiation in the leaf axil. Recent studies also showed that formation of functional tiller buds in rice (Oryza sativa) requires the rice ortholog of WUS (Lu et al, 2015;Tanaka et al, 2015). In Arabidopsis wus mutants, leaf-like structures or even-terminal branches can still form in a portion of leaf axils (23% in wus-1; Figure 1C).…”

Section: Wus Is Required For Am Initiation and Integritymentioning

confidence: 94%

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

et al. 2017

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The homeodomain transcription factor WUSCHEL (WUS) defines the shoot stem cell niche, but the mechanisms underlying the establishment of WUS expression remain unclear. Here, we show that cytokinin signaling precedes WUS expression in leaf axils and activates WUS expression de novo in the leaf axil to promote axillary meristem initiation. Furthermore, type-B Arabidopsis response regulator proteins, which are transcriptional activators in the cytokinin signaling pathway, directly bind to the WUS promoter and activate its expression. Finally, we show that cytokinin activation of WUS in the leaf axil correlates with increased histone acetylation and methylation markers associated with transcriptional activation, supporting the fact that WUS expression requires a permissive epigenetic environment to restrict it to highly defined meristematic tissues. Taken together, these findings explain how cytokinin regulates axillary meristem initiation and establish a mechanistic framework for the postembryonic establishment of the shoot stem cell niche.

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Section: Wus Is Required For Am Initiation and Integritymentioning

confidence: 94%

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

et al. 2017

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“…MOC2 , encodes cytosolic fructose-1, 6-bisphosphatase 1 (FBP1), is essential for the outgrowth of tiller buds in rice [6]. MOC3 , in which a point mutation causes the premature termination of OsWUS , is required for the formation of axillary buds of rice [7]. During the past decade, a series of EMS-induced reduced culm number ( rcn ) mutants and their responsible genes have been characterized in rice.…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice

Liu

Meng

et al. 2017

PLoS ONE

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Tiller number is one of the key factors that influences rice plant type and yield components. In this study, an EMS-induced rice tiller suppression mutant ts1 was characterized. Morphological and histological observations revealed that, in the ts1 plants, the tiller buds were abnormally formed and therefore cannot outgrow into tillers. With an F2 population derived from a cross between ts1 and an indica cultivar Wushansimiao, a major gene, tiller suppression 1 (ts1) was fine-mapped to a 108.5 kb genomic region between markers ID8378 and SSR6884 on the short arm of rice chromosome 2. Candidate gene analysis identified nineteen putative genes. Among them, ORF4 (LOC_Os02g01610) is a PPR gene which harbored a point mutation c.+733/C→T in ts1 mutant plants. A co-dominant SNP marker cd-733C/T was subsequently developed and the SNP assay demonstrated that the point mutation co-segregated with tiller suppression phenotype. Quantitative RT-PCR analysis showed that the expression level of ORF4 in ts1 plants was significantly lower than that in their wild plants, and the expression of rice tillering regulators MOC1 and HTD1 was also significantly decreased in ts1 plants. Our data indicated that ORF4 was a strong candidate gene for ts1 and ts1 might play a role in regulating rice tillering through MOC1 and HTD1 associated pathway. The results above provide a basis for further functional characterization of ts1 and will shed light on molecular mechanism of rice tillering. The informative SNP marker cd-733C/T will facilitate marker-assisted selection of ts1 in rice plant type breeding.

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“…

by which a key tillering regulator (i.e., MOC1) links the cellcycle machinery to regulate tiller bud patterning.Yet the challenge, as this new paper (Lu et al, 2015) also brings to the fore, lies in understanding how OsWUS and MOC1 act in tiller bud cells. It is also of great interest to figure out the genetic and functional relationship of OsWUS, MOC1 and other tillering regulators including the phytohormones, like auxin, cytokinins and strigolactones.

…”

mentioning

confidence: 98%

“…Therefore, the stem-cell origin and molecular regulation of tiller bud formation remain poorly understood. In page 71e78 of this issue (Lu et al, 2015), Jiayang Li group describes an exciting finding that MONO-CULM3 (MOC3), the rice ortholog of WUS, is required for the formation of tiller buds. Molecular genetic studies revealed that MOC3 encodes OsWUS and moc3 is a new rice monoculm mutant in which the tiller bud formation was disrupted.…”

mentioning

confidence: 99%

“…The Li laboratory's work (Lu et al, 2015) comes in the wake of recent reports that several other members of the rice WOX family proteins regulate different aspects of development by determining cell fate and meristem activity. Together, these observations suggest that the WOX proteins in rice have a generally conserved, yet species-specific, function in regulating plant development compared to their Arabidopsis orthologs.…”

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confidence: 99%

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Toward Understanding the Stem-Cell Origin and Molecular Regulation of Rice Tillering

2015

Journal of Genetics and Genomics

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MONOCULM 3, an Ortholog of WUSCHEL in Rice, Is Required for Tiller Bud Formation

Cited by 115 publications

References 39 publications

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

Cytokinin Signaling Activates WUSCHEL Expression during Axillary Meristem Initiation

Fine Mapping and Candidate Gene Analysis of the Tiller Suppression Gene ts1 in Rice

Toward Understanding the Stem-Cell Origin and Molecular Regulation of Rice Tillering

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