Monoclonal antibody to dengue capsid protein

Abstract

Dengue fever (DF) and dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) are considered the most important arthropod-borne viral diseases in terms of morbidity and mortality. The emergency and severity of dengue (Den) infections increase the necessity of an early, quick and effective dengue laboratory diagnostic. Viral isolation is considered a gold standard for diagnosis of dengue infection using monoclonal antibodies (mAbs) as a tool for determining serotype specificity. Alternatives have been used to improve sensitivity and time to dengue diagnosis. Based on the early expression of dengue C protein in the life cycle, we focused our study on the application of an anti-dengue 2 virus capsid protein mAb in dengue diagnosis. The kinetic expression of dengue-2 capsid in mosquito cells and its immuno-localization in experimentally infected suckling albin Swiss (OF-1) mice brain tissues was established. The results demonstrate the possible utility of this mAb in early dengue diagnosis versus traditional isolation. In addition, a preliminary study of an enzyme immunoassay method using 8H8 mAb for specific detection of dengue C protein antigen was performed, making possible recombinant C protein quantification. The results suggest that detection of dengue capsid protein could be useful in the diagnosis of early dengue infection.

Elevated serum levels of high mobility group box 1 (HMGB1) protein in dengue-infected patients are associated with disease symptoms and secondary infection

“…All sera were collected from adult patients. Different methodologies, such as isolation and viral identification by immunofluorescent assays employing serotype-specific monoclonal antibodies, PCR and detection of IgM/IgG, [21][22][23][24] were employed in dengue diagnosis. The IgG levels were used to define primary and secondary cases.…”

Monoclonal antibody against Saint Louis encephalitis prM viral protein

“…The majority of mAbs have been generated to flavivirus envelope (E) protein (Henchal et al, 1989;Gentry et al, 1982;Roehrig et al, 1998); however, mAb specific for capsid and prM protein in mice and humans have been reported (Vásquez et al, 2009;Kaufman et al, 1989;Dejnirattisai et al, 2010). mAbs generated against prM, prM/M or prM/E flavivirus proteins versus Japanese encephalitis and West Nile virus have been useful in seroepidemiological studies to determine which flaviviruses have circulated in a community where more than one flavivirus cocirculates and to analyze the antigenic structure of WNV prM and its contribution Fig.…”

Development and Characterization of Monoclonal Antibodies to Yellow Fever Virus and Application in Antigen Detection and IgM Capture Enzyme-Linked Immunosorbent Assay

“…One of the major challenges in YF laboratory diagnosis is the lack of availability of commercial diagnostic kits. The use of flavivirus antigens in the development of MAbs and diagnostic tests has been documented with very little focus on YF ( 22 , 23 , 25 – 28 , 39 – 42 ). The application of MAbs in immunoassays offers the advantage of high specificity due to their ability to bind to specific epitopes of the antigen.…”

“…A number of antibody (Ab)-based diagnostic tests have been developed for detection of dengue virus (DENV), West Nile virus (WNV), and Japanese encephalitis virus (JEV) using monoclonal and polyclonal antibodies ( 22 – 27 ). However, not much has been reported on the development of such diagnostic tests for YFV ( 28 ).…”