Monoclonal antibody to cytokeratin for use in routine histopathology.

Makin, C. A.; Bobrow, Lynda G.; Bodmer, W F

doi:10.1136/jcp.37.9.975

Cited by 435 publications

(151 citation statements)

References 16 publications

Supporting

Mentioning

141

Contrasting

Unclassified

Order By: Relevance

“…Sections from the same tissue pieces were also stained with an anti-cytokeratin antibody (K903), from Boehringer Mannheim Biochemicals (Indianapolis IN). This antibody recognizes high molecular weight keratins expressed by normal basal epithelial cells in the prostate but not expressed in prostate carcinoma cells (Makin et al, 1984). Additional sections were stained with an antibody to the proliferation-associated antigen, Ki-67 (Key et al, 1993).…”

Section: Organ Culture Procedures and Assessmentmentioning

confidence: 99%

“…Staining with the anti-keratin antibody, K903, revealed strong reactivity in virtually all of the hyper-proliferative glands, as well as in the cells which migrated out from the glands ( Figure 2C). Reactivity of the proliferating cells with this antibody, which is known to react with basal epithelial cells but not with malignant cells (Makin et al, 1984), indicates that the proliferating cells were derived from the non-malignant epithelial cell component present in the original tissue. Consistent with what we have reported in the past , the vast majority of histologically abnormal, K903-negative cells (presumably tumour) were lost from the tissue after 8 days in organ culture under the conditions employed here.…”

mentioning

confidence: 92%

“…Epithelial cells were defined on the basis of cobblestone morphology. Normal basal epithelial cells and malignant epithelial cells were distinguished on the basis of reactivity with anti-keratin antibody K903 (Makin et al, 1984). Virtually all of the epithelial cells that grew in monolayer culture were strongly positive (indicative of normal basal cells).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Matrix metalloproteinases (MMPs) in fresh human prostate tumour tissue and organ-cultured prostate tissue: Levels of collagenolytic and gelatinolytic MMPs are low, variable and different in fresh tissue versus organ-cultured tissue

et al. 2001

View full text Add to dashboard Cite

Summary Prostate tissue was obtained from 22 radical prostatectomies (performed for clinical management of prostate carcinoma) immediately after surgery. A small piece of tissue was fixed immediately in formalin and used for routine histology while a second piece was frozen in OCT and used for immuno-histochemistry. Another small piece was used for isolation of epithelial and stromal cells. The remainder of the tissue was cut into 2 × 2 mm pieces and incubated in organ culture for 8 days. In organ culture, non-malignant, basal epithelial cells underwent a proliferative response. This was accompanied by de-differentiation of glandular structures and by migration of epithelial cells across the surface of the tissue. Erosion of the basement membrane could also be seen in places, but was not widespread. Invasion of epithelial cells into the adjacent stroma was not evident. Production of matrix metalloproteinases (MMPs) with gelatinolytic activity or collagenolytic activity was assessed in organ culture and compared to expression patterns in fresh tissue. MMP-1 (interstitial collagenase) and MMP-9 (92-kDa gelatinase B) were undetectable or low in fresh tissue specimens. Both enzymes were detected in organ culture and both increased over time. Even after 6 days, however, there was only a low level of gelatin-hydrolytic activity and no measurable collagenhydrolytic activity. In past studies we used organ cultures of normal skin and malignant skin tumours (basal cell carcinomas) to help elucidate the role of collagenolytic and gelatinolytic MMPs in epithelial cell invasion (Varani et al, 2000). Compared to MMP levels observed in skin, levels of these enzymes in prostate are low. The low level of collagenolytic and gelatinolytic MMPs in fresh prostate tissue and in organcultured prostate tissue may help explain why there is little tissue destruction in many primary prostate tumours and why the majority of such tumours remain confined to the prostate for extended periods. Although these past studies clearly indicate that several MMPs are elaborated in prostate tumours and suggest differences between malignant and non-malignant tissue, the heterogeneity in prostate tumour presentation and the variability in the analytical methods used make elucidating the relationship between enzyme elaboration and disease-state difficult to ascertain. Furthermore, the presence of active forms of the enzymes has not been established in these studies, and the biological significance of the reported differences is unclear. In the present study, a combination of approaches has been employed to assess expression of collagenolytic and gelatinolytic MMPs in prostate tissue removed for treatment of prostate carcinoma. We report here that gelatinolytic and collagenolytic MMPs can be detected in freshly isolated prostate tissue or in short-term (2-3 day) organ culture. Expression is heterogeneous from tissue to tissue, consistent with the variation in disease presentation. Expression patterns in fresh tissue and organ-cultured tissue also differ. E...

show abstract

Section: Organ Culture Procedures and Assessmentmentioning

confidence: 99%

mentioning

confidence: 92%

See 1 more Smart Citation

Matrix metalloproteinases (MMPs) in fresh human prostate tumour tissue and organ-cultured prostate tissue: Levels of collagenolytic and gelatinolytic MMPs are low, variable and different in fresh tissue versus organ-cultured tissue

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Epithelial cells within the tumour were stained with Cam 5.2, a MAb that reacts with secretory epithelia of normal tissue as well as epithelial adenocarcinomas (Makin et al, 1984).…”

Section: Identification Of Tumour Cellsmentioning

confidence: 99%

Immunohistochemical localization of CD1a-positive putative dendritic cells in human breast tumours

1999

View full text Add to dashboard Cite

SummaryThe presence of a high number of infiltrating CD1a + cells in malignant neoplasms has been reported to be associated with an improved prognosis, reduced tumour recurrence and fewer metastases. This study identified a population of CD1a + cells within the lymphoid cell infiltrate in human ductal breast carcinoma (n = 52), which was significantly different from normal breast tissue, in which only two out of nine cases expressed CD1a + cells (P = 0.0192). In the majority of cases, the infiltrate was low compared with the number of macrophages and T cells present (results not shown). There was no correlation between the number of CD1a + cells and tumour grade, with all tumour grades expressing similar numbers of infiltrating CD1a + cells. There was clear evidence, however, that the CD1a + cells were closely associated with tumour cells. It is likely that CD1a + cells have a role in antigen capture and presentation in human tumours, and this study documents the density of CD1a + cells in a large sample of all histological grades of human breast carcinomas.

show abstract

“…Results from serial sections (3 -4 mm) of two representative biopsy specimens within the Checkerboard TMA containing cancer stained by H&E, PSA, CAM 5.2 (low-molecular-weight keratin) and LP34 (high-molecularweight keratin) are shown (Figures 2A and B). The PSA, CAM 5.2 and LP34 antibodies are used routinely in the diagnosis of prostate cancer (Moll et al, 1982;Makin et al, 1984;Gillespie et al, 2002;Evans, 2003). An interesting feature of the Checkerboard TMA analyses was that cancer was identified in nine out of 19 (five out of 19 based on H&E staining alone) checkers containing biopsy specimens originally judged as normal.…”

Section: Analysis Of Ihc Markers Using Checkerboard Tmasmentioning

confidence: 99%

Construction of tissue microarrays from prostate needle biopsy specimens

et al. 2005

View full text Add to dashboard Cite

Needle biopsies are taken as standard diagnostic specimens for many cancers, but no technique exists for the high-throughput analysis of multiple individual immunohistochemical (IHC) markers using these samples. Here we present a simple and highly reliable technique for constructing tissue microarrays (TMAs) from prostatic needle biopsies. Serial sectioning of the TMAs, called 'Checkerboard TMAs', facilitated expression analysis of multiple proteins using IHC markers. In total, 100% of the analysed biopsies within the TMA both preserved their antigenicity and maintained their morphology. Checkerboard TMAs will allow the use of needle biopsies (i) alongside other tissue specimens (trans-urethral resection of prostates and prostatectomies in the case of prostate cancer) in clinical correlation studies when searching for new prognostic markers, and (ii) in a diagnostic context for assessing expression of multiple proteins in cancers from patients prior to treatment.

show abstract

Monoclonal antibody to cytokeratin for use in routine histopathology.

Cited by 435 publications

References 16 publications

Matrix metalloproteinases (MMPs) in fresh human prostate tumour tissue and organ-cultured prostate tissue: Levels of collagenolytic and gelatinolytic MMPs are low, variable and different in fresh tissue versus organ-cultured tissue

Matrix metalloproteinases (MMPs) in fresh human prostate tumour tissue and organ-cultured prostate tissue: Levels of collagenolytic and gelatinolytic MMPs are low, variable and different in fresh tissue versus organ-cultured tissue

Immunohistochemical localization of CD1a-positive putative dendritic cells in human breast tumours

Construction of tissue microarrays from prostate needle biopsy specimens

Contact Info

Product

Resources

About