Monoclonal antibody studies of ferredoxin:NADP+ oxidoreductase

Chang, Kai-Tai; Morrow, K. John; Hirasawa, Masakazu; Knaff, David B.

doi:10.1016/0003-9861(91)90576-5

Cited by 11 publications

(4 citation statements)

References 29 publications

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“…In contrast, the rice leaf FNR has a strong similarity to other leaf FNR ( > 80 7o), than to the rice root FNR. These data are consistent with the presence of enzymatically and immunochemically distinct FNR in roots and leaves [5,8,15,19,31].…”

Section: Homology Of Zmrprnl To the Rice Root Ferredoxin Nadp + Reducsupporting

confidence: 78%

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Identification of a maize root transcript expressed in the primary response to nitrate: characterization of a cDNA with homology to ferredoxin-NADP+ oxidoreductase

et al. 1994

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To more fully understand the biochemical and molecular events which occur in plants exposed to nitrate, cDNAs whose accumulation was enhanced in nitrate- and cycloheximide-treated maize (Zea mays L. W64A x W182E) roots were isolated. The 340 bp Zmrprn1 (for Zea mays root primary response to nitrate) cDNA also hybridized with a probe enriched for nitrate-induced sequences, and was characterized further. Sequence analysis of a near full-length cDNA (Zmrprn1A) showed strong homology (> 90% amino acid identity) with a root ferredoxin-NADP+ oxidoreductase (FNR) of rice, and 45-50% amino acid identify with leaf FNR genes. When expressed in Escherichia coli, the Zmrprn1A cDNA produced a protein with NADPH: ferricyanide reductase activity, consistent with the enzymatic properties of an FNR. The Zmrprn1 cDNA hybridized with a 1.4 kb transcript which was expressed in the maize root primary response to nitrate. That is, mRNA levels in roots increased rapidly and transiently in response to external nitrate, and low levels of nitrate (10 microM) induced transcript accumulation. The accumulation of the Zmrprn1 transcript was not prevented by cycloheximide, indicating that the cellular factor(s) required for expression were constitutively present in maize roots. The Zmrprn1 mRNA accumulated specifically in response to nitrate, since neither K+ nor NH4+ treatment of roots caused transcript accumulation. Maize leaves had about 5% of the transcript level found in roots, indicating a strong preference for expression of Zmrprn1 in roots. Analysis of maize genomic DNA indicated the presence of only a single gene or very small gene family for the Zmrprn1. Together, the data indicate that Zmrprn1A encodes a nitrate regulated maize root FNR.

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Section: Homology Of Zmrprnl To the Rice Root Ferredoxin Nadp + Reducsupporting

confidence: 78%

“…In non-green tissues, plastid Fd-NADP + oxidoreductase (FNR) is believed to use N A D P H to reduce Fd [8,19,31,32]. This is in contrast to the chloroplastic FNR, which uses reduced Fd generated from photosystem I to reduce NADP + [25].…”

Section: Introductionmentioning

confidence: 94%

Identification of a maize root transcript expressed in the primary response to nitrate: characterization of a cDNA with homology to ferredoxin-NADP+ oxidoreductase

et al. 1994

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“…We hypothesised, that three distinct sites at FNR molecule are involved in binding either NADP þ (H), Fd, or quinone, and quinone binding site at the membrane-anchored FNR molecule participates in the cyclic electron flow around photosystem 1 [20]. The earlier data, indicating that monoclonal antibodies against FNR inhibit the dichlorophenol indophenol reduction but have no effect on the enzyme activity in the presence of Fd and cytochrome c as an electron acceptor system [21], are consistent with this proposal. The three substrate binding sites have further been supported by the studies on respondents of the enzyme to selective inhibitors [20,22].…”

Section: Introductionmentioning

confidence: 94%

Effect of cadmium on ferredoxin:NADP+ oxidoreductase activity

Grzyb

Waloszek

Latowski

et al. 2004

Journal of Inorganic Biochemistry

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“…The first one is located in the domain at the C-terminus, whereas the second one was found in a cleft between two domains in the proximity to the FAD (Karplus et al 1991, Kurisu et al 2001. Chang et al (1991) found that the binding site involved in Fd-Cyt c reduction differs from that in-volved in the DCPIP reduction. They isolated eleven monoclonal antibodies IgG's which specifically blocked DCPIP reduction, but had no effect on the Fd-dependent Cyt c reduction.…”

mentioning

confidence: 91%

Effects of Amino and Thiol Group Reagents on the Ferredoxin:NADP<sup>+</sup> Oxidoreductase Catalysed Reduction of Dibromothymoquinone

Grzyb¹,

Bojko²,

Więckowski³

2003

Photosynt.

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Effects of selective reagents of amino groups (fluorescamine, Fc) and thiol [5,5'-dithio-bis(2-nitrobenzoic) acid, DTNB] groups on the diaphorase activity of spinach ferredoxin:NADP + oxidoreductase (FNR, E.C 1.18.1.2) in the presence of dibromothymoquinone (DBMIB) as an electron acceptor were studied. The incubation of FNR with 250 µM Fc in the time range from 0 to 120 min led to the gradual decrease of FNR activity according to biphasic kinetics. At the initial phase the activity (defined as the rate of NADPH oxidation) decreased about 4-time faster than at the subsequent second slower phase. Incubation of FNR simultaneously with Fc and DBMIB for more than 20 min caused restoration of the activity to about 80 % of the control. The inhibitory effect of Fc on the FNR-catalysed DBMIB reduction had non-competitive character. Incubation of FNR with DTNB led also to a gradual decrease of the enzyme activity, which reached about 45 % of the control after 2 h of incubation. Thus neither amino nor thiol groups in the FNR molecule are involved directly in the DBMIB reduction. However, the presence of DBMIB in the incubation medium influenced the inhibitory pattern of Fc and DTNB, and this suggests that DBMIB modified the conformational state of the FNR molecule.

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Monoclonal antibody studies of ferredoxin:NADP+ oxidoreductase

Cited by 11 publications

References 29 publications

Identification of a maize root transcript expressed in the primary response to nitrate: characterization of a cDNA with homology to ferredoxin-NADP+ oxidoreductase

Identification of a maize root transcript expressed in the primary response to nitrate: characterization of a cDNA with homology to ferredoxin-NADP+ oxidoreductase

Effect of cadmium on ferredoxin:NADP+ oxidoreductase activity

Effects of Amino and Thiol Group Reagents on the Ferredoxin:NADP<sup>+</sup> Oxidoreductase Catalysed Reduction of Dibromothymoquinone

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