Monoclonal antibody (SBU-1 and SBU-3) identification of cells dissociated from the sheep placentomal trophoblast.

Lee, C S; Ralph, M. M.; Gogolin-Ewens, K J; Brandon, Malcolm R.

doi:10.1177/38.5.1692053

Cited by 11 publications

(5 citation statements)

References 9 publications

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“…These results indicate that the process of losing basal contact does not have to be completed before the cells become binucleate. Findings of previous studies showed that alpha-keratin is localized in basally located binucleate cells, but not in mature, apically migrated cells [Lee et al, 1990]. This coincides with our results, as cytokeratin constitutes an important intracellular anchorage of hemidesmosomes, which attach epithelial cells to the BM [Alberts et al, 2015].…”

Section: Discussionsupporting

confidence: 80%

Morphological Characterization of Basally Located Uninucleate Trophoblast Cells as Precursors of Bovine Binucleate Trophoblast Giant Cells

Attiger¹,

Boos²,

Klisch³

2018

Cells Tissues Organs

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Binucleate trophoblast giant cells (TGCs) are one characteristic feature of the ruminant placenta. In cows, the frequency of TGCs remains constant for most of the duration of pregnancy. As TGCs are depleted by their fusion with uterine epithelial cells, they need to be constantly formed. It is still unclear whether they develop from stem cells within the trophectoderm or whether they can arise from any uninucleate trophoblast cell (UTC). Within the latter, generally accepted theory, a basally located uninucleate cell (BUC) without contact to the feto-maternal interface would represent a transient cell between a UTC and a TGC. So far, no evidence for the existence of such transient cells or for the presence of stem cells has been shown. The aim of the present study is to morphologically characterize the early stages of TGC development. Placentomal tissue of 6 pregnant cows from different gestational stages (gestational days 51–214) was examined for BUCs, UTCs, and TGCs either in serial sections (light and transmission electron microscopy, TEM, n = 3), in single sections (TEM, n = 2), or by serial block face-scanning electron microscopy (n = 1). These investigations revealed the occurrence of BUCs, as well as young TGCs showing contact with the basement membrane (BM), but without apical contact to the feto-maternal interface. The study morphologically defines these 2 cell types as early stages of TGC development and shows that binucleation of TGCs can precede detachment from the BM.

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Section: Discussionsupporting

confidence: 80%

Morphological Characterization of Basally Located Uninucleate Trophoblast Cells as Precursors of Bovine Binucleate Trophoblast Giant Cells

Attiger¹,

Boos²,

Klisch³

2018

Cells Tissues Organs

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“…Migrated binucleate cells are free from the basal lamina, but keep a tight junction with adjacent cells . Such migratory behavior of binucleate cells is associated with altered expression of cytokeratin (Lee et al 1990;Nakano et al 2001) and β 1 -integrin (MacLaren and Wildeman 1995).…”

Section: Discussionmentioning

confidence: 99%

Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen

et al. 2001

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The differentiation of trophectoderm in ruminants is marked by the appearance of binucleate cells in cytotrophoblasts. Binucleate cells are produced by the acytokinesis of cytotrophoblasts and undergo endoreduplication. They secrete hormones such as placental lactogen, and exhibit migratory behavior to transfer their hormones into maternal circulations. In this study, we showed that a bovine trophoblastic cell line (BT-1) established from in vitro fertilized blastocysts differentiated into binucleate cells on collagen gel. BT-1 had cytotrophoblastic epithelial characteristics in that it expressed cytokeratin, E-cadherin and interferon-tau. It spontaneously formed multicellular spherical vesicles floating in the medium. We cultured these vesicles on type I collagen substrata. Most vesicles attached to the collagen substrata, and exhibited cell outgrowth and proliferation. We found that after more than 10 days, clusters of binucleate cells appeared in the cell colonies on the collagen gel, but not on the collagen film. These binucleate cells have features characteristic of those in vivo, including an increased nuclear DNA content and the expression of placental lactogen. BT-1 is a useful model with which to study trophoblast differentiation in ruminants.

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“…It is possible that binucleate cells lose their capacity to express cytokeratin during the course of differentiation. Further evidence supporting this hypothesis is that in ovine placentomes the anti-SBU-1 antibody (an anti-cytokeratin antibody) stains mononucleate epithelial cells and young binucleate cells at the trophectoderm basal lamina, but not binucleate cells in the apical region of trophectoderm epithelial sheets (Lee et al 1990). The study of bovine placenta tissue sections will confirm our results in vitro experiments.…”

Section: Discussionmentioning

confidence: 94%

“…The study of Lee et al (1990) is also of note that binucleate cells in migration are devoid of cytokeratin staining. Cytokeratin filaments are anchored to desmosomes and hemidesmosomes through which cell-to-cell and cell-to-matrix connections are performed.…”

Section: Discussionmentioning

confidence: 96%

Expression of placental lactogen and cytokeratin in bovine placental binucleate cells in culture

Nakano

Takahashi

Imai

et al. 2001

Cell and Tissue Research

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Binucleate cells are present in ruminant placenta and play an endocrine role in the production of many hormones during pregnancy. We isolated and cultured binucleate cells from bovine placenta at middle to late gestation and characterized these cells using immunofluorescence techniques. Enriched preparations of binucleate cells were obtained using Percoll density gradient centrifugation following collagenase digestion. Binucleate cells in culture preferentially attached to collagen-coated dishes rather than to noncoated plastic dishes. The cells gradually extended their edges on collagen substrata, and finally assumed a flattened morphology. Antibodies to placental lactogen (PL) and pregnancy-associated glycoprotein-1 (PAG-1) specifically stained the majority of round binucleate cells, but not the flat cells. We found that PL-positive binucleate cells were consistently devoid of cytokeratin. In contrast, cytokeratin was expressed in PL-negative binucleate cells as well as mononuclear epithelial cells. Furthermore, the PL-negative flat binucleate cells also developed intense cytokeratin networks in the cytoplasm. These results indicate that cytokeratin expression is inversely proportionate to that of PL in cultured binucleate cells. We conclude that downregulation of cytokeratin in binucleate cells is a function of the state of cellular differentiation.

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Monoclonal antibody (SBU-1 and SBU-3) identification of cells dissociated from the sheep placentomal trophoblast.

Cited by 11 publications

References 9 publications

Morphological Characterization of Basally Located Uninucleate Trophoblast Cells as Precursors of Bovine Binucleate Trophoblast Giant Cells

Morphological Characterization of Basally Located Uninucleate Trophoblast Cells as Precursors of Bovine Binucleate Trophoblast Giant Cells

Bovine trophoblastic cell differentiation on collagen substrata: formation of binucleate cells expressing placental lactogen

Expression of placental lactogen and cytokeratin in bovine placental binucleate cells in culture

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