Monoclonal antibody-recognizing cone visual pigment

Szél, Ágoston; Takács, László; Monostori, Éva; Diamantstein, Tibor; Vigh-Teichmann, I.; Röhlich, P.

doi:10.1016/0014-4835(86)90066-7

Cited by 97 publications

(37 citation statements)

References 25 publications

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“…1B). GCAP1 colocalized with cone pigments when frozen sections were double stained with COS-1 (specific for M-cone pigment) and OS-2 (UV-cone pigment) (27). The staining of G1T3 ϩ GCAP Ϫ/Ϫ cones with UW-14 (GCAP1) seems uniform.…”

Section: Expression Of Transgenic Gcap1 In Conesmentioning

confidence: 95%

Guanylate cyclase-activating protein (GCAP) 1 rescues cone recovery kinetics in GCAP1/GCAP2 knockout mice

Pennesi

Howes

Baehr

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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Mediated by guanylate cyclase-activating proteins (GCAPs), cytoplasmic Ca 2؉ levels regulate the activity of photoreceptor guanylate cyclase (GC) and the synthesis of cGMP, the internal transmitter of phototransduction. When GCAP1 is expressed in transgenic mice on a GCAP null background, it restores the wild-type flash responses in rod photoreceptors. In this communication, we explored the role of GCAP1 in cone photoreceptors by using electroretinograms (ERGs). Under cone isolation conditions, ERGs recorded from mice lacking both GCAP1 and GCAP2 had normal amplitudes of the saturated a-wave and b-wave. However, recordings from these mice demonstrated a widened b-wave and increased sensitivity of both M-and UV-cone systems. Paired-flash ERGs revealed a delayed recovery of both the cone driven b-wave and a-wave and suggest that the delay originated from the photoreceptors. To test whether GCAP1 could restore normal cone response recovery, mice that expressed only transgenic GCAP1 in the absence of wild-type GCAP expression were tested. Immunohistochemical analysis demonstrated that cones of these mice expressed high levels of GCAP1. Paired-flash ERGs showed that the recovery of the cone-driven a-wave was restored to normal, whereas recovery of the cone-driven b-wave was slightly faster than that observed in wild-type mice. These studies reveal that, similar to rods, deletion of GCAP1 and GCAP2 delays the recovery of light responses in cones and GCAP1 restores the recovery of cone responses in the absence of GCAP2.

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Section: Expression Of Transgenic Gcap1 In Conesmentioning

confidence: 95%

Guanylate cyclase-activating protein (GCAP) 1 rescues cone recovery kinetics in GCAP1/GCAP2 knockout mice

Pennesi

Howes

Baehr

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

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“…Since bovine rhodopsin could be isolated by biochemical methods, an early approach was to raise polyclonal sera (Jan and Revel, 1974;Papermaster et al, 1978) or monoclonal antibodies against rhodopsin and subsequently identify the epitopes by blocking with synthetic peptides (Adamus et al, 1988;Fekete and Barnstable, 1983;MacKenzie and Molday, 1982;Molday and MacKenzie, 1983;Molday, 1989;Witt et al, 1984). The monoclonal anti-body technique was also used to fish out from the many hybridoma supernatants (resulting from mice immunized with retinal membranes) those that contained antibodies selectively staining certain cone types (Szél et al, 1986). A subsequent laborious work had to be performed until the spectral type of the labeled photoreceptor, as well as the molecular nature of the epitope, could be identified (Röhlich and Szél, 1993;Szél et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Photoreceptor cells in the Xenopus retina

Röhlich¹,

Szél

2000

Microsc. Res. Tech.

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“…D. S. PAPERMASTER, New Haven, Ct, USA: CONVERSE and PAPERMASTER, 1975;PAPERMASTER, 1982) and the rat (Dr. P. ROHLICH, Budapest: SzEL et al, 1985). The specificity of the latter antiserum was tested by immunoblotting (SzEL et al, 1986b). The primary 497 antisera were diluted appropriately (1:100 to 1: 250,000) in phosphate-buffered saline (PBS)-O.5%' human serum albumin (HSA) of pH 7.4 for up to 48 hr at 4°C.…”

Section: Methodsmentioning

confidence: 99%