Monoclonal antibody production to human and bovine 2′:3′-cyclic nucleotide 3′-phosphodiesterase (CNPase): high-specificity recognition in whole brain acetone powders and conservation of sequence between CNP1 and CNP2

Sprinkle, T.J.; Agee, Julia F.; Tippins, Russell B.; Chamberlain, C R; Faguet, Guy B.; Vries, George H. De

doi:10.1016/0006-8993(87)90888-2

Cited by 42 publications

(17 citation statements)

References 23 publications

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“…The neuronal markers neurofilament light isoform, microtubule associated protein 2C, tau, nonphosphorylated neurofilament heavy isoform (SMI32), neuronspecific enolase, and tyrosine hydroxylase were weakly expressed in many of the cultures, with occasionally stronger expression of tau and SMI32 when cultures SL and LU2 were grown in EGM2MV media on human extracellular matrix or on tissue culture plastic. CNPase and galactocerebroside are specifically expressed in oligodendrocytes and Schwann cells (18,19). Both markers were strongly expressed in most of the EBD cultures.…”

Section: ϫ5mentioning

confidence: 94%

Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro

Shamblott¹

2000

Proceedings of the National Academy of Sciences

120

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Section: ϫ5mentioning

confidence: 94%

Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro

Shamblott¹

2000

Proceedings of the National Academy of Sciences

120

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“…The CNPase activity purified from wheat germ immunostains with a polyvalent anti-CNPase antiserum prepared against mammalian brain CNPase. The enzyme preparation from wheat germ comigrated with CNPl and CNP2 of various mammalian brain CNPases (Sprinkle et al, 1988). CNPase and a cholesterol ester hydrolase with a pH optimum of 7.2 (EC 3.1.1.13 .)…”

Section: Introductionmentioning

confidence: 99%

Glycerophosphorylcholine phosphocholine phosphodiesterase activity in cultured oligodendrocytes, astrocytes, and central nervous tissue of dysmyelinating rodent mutants

Yuan

McCartney

Monge

et al. 1992

J of Neuroscience Research

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The levels of GPC phosphocholine phosphodiesterase, pNP phosphocholine phosphodiesterase, CNPase, and UDP galactose: ceramide galactosyltransferase activities were estimated with pure cultures of oligodendrocytes and astrocytes; mixed primary glial cells cultures; C-6 cells; and CNS tissue of the dysmyelinating md rat, the jimpy mouse, and the quaking mouse. The highest activity of GPC and pNP phosphocholine phosphodiesterases as with CNPase and C gal T was found in the pure cultured oligodendrocytes. C-6 cells had very low or undetectable activities for these two phosphodiesterases but possessed very high CNPase activity. The activity of GPC phosphocholine phosphodiesterase was significantly decreased in the CNS tissue of the md rat and the jimpy and the quaking mouse. Similar reductions were observed for the pNP phosphocholine phosphodiesterase, CNPase, and C gal T activities. The selective cellular enrichment in oligodendrocytes of the GPC phosphocholine phosphodiesterase activity and decreases of its activity in three dysmyelinating mutants in the same ratio as for CNPase and C gal T suggest that GPC phosphocholine phosphodiesterase is a myelin marker enzyme and it may reflect the quantity of myelin and oligodendrocyte present.

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“…For instance, platelet-derived growth factor (PDGF) alpha receptor (Pringle and Richardson, 1993;Oumesmar et al, 1997), monoclonal antibody A2B5 (Eisenbarth et al, 1979), and the rat NG2 proteoglycan (Dawson et al, 2000) are present in early oligodendrocyte precursor cells, although some of them are also present in other glial or neuronal cell types. Many myelin genes (Jordan et al, 1989) or antigenic markers for myelin/oligodendrocyte-specific components such as monoclonal antibody O4 (Sommer and Schachner, 1981), galactocerebrosides (GalC) , proteolipid protein isoform DM20 (Dickinson et al, 1996), 2Ј,3Ј-cyclic nucleotide-3Ј-phohydrolase (CNP; Sprinkle et al, 1987), and myelin basic protein (MBP; Kies et al, 1972) recognize both myelin and oligodendrocyte cell bodies. In the present study, we used a specific ABCA2-antibody combined with several selected glial markers to demonstrate that ABCA2 begins to be expressed in spinal cord oligodendrocyte progenitors in situ and continues to be expressed into adulthood in mature oligodendrocytes during postnatal development of rats.…”

mentioning

confidence: 99%

ATP‐binding cassette transporter ABCA2 (ABC2) expression in the developing spinal cord and PNS during myelination

Zhou

Inagaki

Pleasure

et al. 2002

J of Comparative Neurology

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We examined developmental characteristics of the ATP-binding cassette transporter ABCA2 (or ABC2) -expressing cells in rat spinal cord and peripheral nerves. In adult spinal cord, ABCA2 immunoreactivity was detected in lysosome-like organelles of mature oligodendrocyte cell bodies, and a single specific band was detected by Western blot analysis. In postnatal developing spinal cord, ABCA2 immunolabeling was first detected in a small number of cells restricted to the ventral marginal area and the dorsal funiculus at birth (P0). ABCA2-positive cells were co-immunolabeled by O4, a marker for late progenitor and immature oligodendrocytes. At the same time, myelin basic protein was apparent in the same restricted regions. The number of ABCA2 and O4 co-immunolabeled cells increased quickly in both dorsal and ventral regions from P2 and reached a peak at P8. After transient expression from P0 to P8, O4 labeling in white matter tracts decreased and disappeared. In contrast, ABCA2-positive oligodendrocytes persisted in gray and white matter throughout the spinal cord into adulthood. These data suggest a role for the ABCA2 transporter in maturation of oligodendrocyte lineage cells and the onset of myelination in the central nervous system. In addition, ABCA2 immunoreactivity was detected in the ciliated region of the ependyma in the central canal from early postnatal development. ABCA2 immunoreactivity was also detected in the Schwann cell lineage in developing spinal nerves and in adult trigeminal and sciatic nerves. ABCA2 was also expressed in numerous undetermined cells distributed in para-nerve connective tissues and nerve sheaths throughout early postnatal development. These data indicate multiple levels of involvement for ABCA2 in nervous system development especially with strong evidence for a role in myelination.

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Monoclonal antibody production to human and bovine 2′:3′-cyclic nucleotide 3′-phosphodiesterase (CNPase): high-specificity recognition in whole brain acetone powders and conservation of sequence between CNP1 and CNP2

Cited by 42 publications

References 23 publications

Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro

Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro

Glycerophosphorylcholine phosphocholine phosphodiesterase activity in cultured oligodendrocytes, astrocytes, and central nervous tissue of dysmyelinating rodent mutants

ATP‐binding cassette transporter ABCA2 (ABC2) expression in the developing spinal cord and PNS during myelination

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