Monoclonal antibody probes discriminate early and late mutant defects in development of the Drosophila retina

Renfranz, Patricia J.; Benzer, Seymour

doi:10.1016/0012-1606(89)90267-4

Cited by 50 publications

(23 citation statements)

References 62 publications

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“…The predominant phenotype of hh 1 is a reduction of eye facets. Therefore, the deletion leading to the hh 1 allele may affect an eye-specific enhancer of hh (Renfranz and Benzer, 1989).…”

Section: Eyeless Is a Direct Target Of Somentioning

confidence: 99%

Identification of functionalsine oculismotifs in the autoregulatory element of its own gene, in theeyelessenhancer and in the signalling genehedgehog

et al. 2005

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In Drosophila, the sine oculis (so) gene is important for the development of the entire visual system, including Bolwig's organ, compound eyes and ocelli. Together with twin of eyeless, eyeless, eyes absent and dachshund, sobelongs to a network of genes that by complex interactions initiate eye development. Although much is known about the genetic interactions of the genes belonging to this retinal determination network, only a few such regulatory interactions have been analysed down to the level of DNA-protein interactions. Previous work in our laboratory identified an eye/ocellus specific enhancer of the sine oculis gene that is directly regulated by eyeless and twin of eyeless. We further characterized this regulatory element and identified a minimal enhancer fragment of so that sets up an autoregulatory feedback loop crucial for proper ocelli development. By systematic analysis of the DNA-binding specificity of so we identified the most important nucleotides for this interaction. Using the emerging consensus sequence for SO-DNA binding we performed a genome-wide search and have thereby been able to identify eyeless as well as the signalling gene hedgehog as putative targets of so. Our results strengthen the general assumption that feedback loops among the genes of the retinal determination network are crucial for proper development of eyes and ocelli.

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“…The predominant phenotype of hh 1 is a reduction of eye facets. Therefore, the deletion leading to the hh 1 allele may affect an eye-specific enhancer of hh (Renfranz and Benzer, 1989).…”

Section: Eyeless Is a Direct Target Of Somentioning

confidence: 99%

Identification of functionalsine oculismotifs in the autoregulatory element of its own gene, in theeyelessenhancer and in the signalling genehedgehog

et al. 2005

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“…A class of mutations including Bar (B) may also be intimately related to early events (9). For clarification of this point, examination was first made of possible determinant genes for the Bar mutation B of Drosophila melanogaster (10) and optic morphology mutation Om(JD) of Drosophila ananassae (11), in both of which ommatidium differentiation is suppressed in the anterior portion of the eye.…”

mentioning

confidence: 99%

Identification of a different-type homeobox gene, BarH1, possibly causing Bar (B) and Om(1D) mutations in Drosophila.

Kojima

Ishimaru

Higashijima

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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The Bar mutation B of Drosophila melanogaster and optic morphology mutation Om(JD) of Drosophila ananassae result in suppression of ommatidium differentiation at the anterior portion of the eye. Examination was made to determine the genes responsible for these mutations. Both loci were found to share in common a different type of homeobox gene, which we call "BarH1." Polypeptides encoded by D. melanogaster and D. ananassae BarHl genes consist of 543 and 604 amino acids, respectively, with homeodomains identical in sequence except for one amino acid substitution. A unique feature of these homeodomains is that the phenylalanine residue in helix 3, conserved in all metazoan homeodomains so far examined, is replaced by a tyrosine residue. By Northern blotting, considerably more BarH1 RNA was detected in the Bar mutant than in wild type. P element-mediated transformation showed Bar-like eye malformation to be induced by transient overexpression of the BarH1 gene in the late thirdinstar larvae. Somatic recombination analysis indicated normal gene functions ofthe Bar region, including the BarH1 gene, to be required for normal eye morphogenesis.

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“…3). These results suggest that the c n o gene product is needed after pupation, as has been implied for the mutations in which the eye discs appear normal through the third instar but the adult eyes are irregular (Renfranz and Benzer 1989;Baker et al 1992), although we cannot exclude the possibility that defects in the c n o mutant eye could be the consequence of abnormalities present in the larval eye disc but too subtle to be detected in our light microscopic studies.…”

Section: Phenotypic Characterization Of Cno Mislmentioning

confidence: 61%

canoe encodes a novel protein containing a GLGF/DHR motif and functions with Notch and scabrous in common developmental pathways in Drosophila.

Miyamoto¹,

Nihonmatsu²,

Kondo³

et al. 1995

Genes Dev.

113

109

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The canoe mistyl (cno ~I~1) mutation was isolated by virtue of its severe rough eye phenotype from ~500 fly lines, each harboring a single autosomal insertion of a P element (BmAw). Excision of the P element generated a lethal, null allele, cno mis~~ together with many revertants with normal eye morphology. Ommatidia homozygous for cno ~s~~ produced in an otherwise wild-type eye by somatic recombination, typically contain a reduced number of outer photoreceptors. Some

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Monoclonal antibody probes discriminate early and late mutant defects in development of the Drosophila retina

Cited by 50 publications

References 62 publications

Identification of functionalsine oculismotifs in the autoregulatory element of its own gene, in theeyelessenhancer and in the signalling genehedgehog

Identification of functionalsine oculismotifs in the autoregulatory element of its own gene, in theeyelessenhancer and in the signalling genehedgehog

Identification of a different-type homeobox gene, BarH1, possibly causing Bar (B) and Om(1D) mutations in Drosophila.

canoe encodes a novel protein containing a GLGF/DHR motif and functions with Notch and scabrous in common developmental pathways in Drosophila.

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