Monoclonal antibody JC1: new reagent for studying cell proliferation.

Abstract

Aim: To characterise a newly developed mouse monoclonal antibody JC1 which recognises a nuclear antigen present in proliferating cells in normal tissues and neoplastic lesions, and which is absent in resting cells. Methods: The methodology was established using a representative range of frozen sections from normal tissues and from certain tumours which were immunostained with antibodies Ki67 and JC1. The molecular weight of the antigen recognised by JC1 was obtained by western blot analysis and this was compared with that of Ki67. IM-9 cell lysates containing Ki67 derived plasmids were also tested with JC1 antibody.

Validation of the S-phase specificity of histone (H3) in situ hybridization in normal and malignant cells.

“…[26][27][28][29][30][31][32][33]. The two proteins that have arguably found the most application to the study of human tumors are the Ki-67 antigen and PCNA (11,34).…”

Imunoexpression of Ki-67 and p53 in Rectal Cancer Tissue After Treatment with Neoadjuvant Chemoradiation

“…The constant increase in the incidence of these cancers in both sexes in recent years makes the knowledge of tumor types and identification of new prognosticators important. Histopathological characterization differs from other tumors and contradictory observations on the prognostic role of Ki-67 proliferative activity were reported [18][19][20][21][22][23][24].…”

“…Ki67 expression is highly present in a great number of tumor cells before any treatment [18,21,22]. In this assay, HPI of Ki67 was expressed in 9.67% of the tumors of the NG and in 33.33% of the tumors of the AG (p=0.052).…”

“…Ki-67 is a nuclear protein present in all cell phases, except for G0 and early G1 phase [17] making it a marker for cycling cells. Ki-67 detected in proliferating cells, in both frozen and paraffinembedded tissue has been related as a prognostic value in colorectal cancer, although this is still controversial [18][19][20][21][22][23][24].…”

From 3H to H3: Slide-Based Analyses of the Cell Cycle.

“…More recently, there have been new monoclonal antibodies generated to as-yet undefined nuclear antigens associated with cell proliferation, and which await further characterization: JC1 [18], which identifies 123 and 212 kd protein, and KiS1, which identifies a 160 kd protein [32].…”