Monoclonal antibody-based sandwich ELISA for the detection of mammalian meats

Jiang, Xingyi; Rao, Qinchun; Mittl, Kristen; Hsieh, Yun-Hwa Peggy

doi:10.1016/j.foodcont.2019.107045

Cited by 22 publications

(8 citation statements)

References 29 publications

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“…In this study, a pair of monoclonal antibodies (MAbs 8F10 and 5H9) were used with Mab, 8F10 specific for skeletal muscle troponin I (TnI) and biotin-conjugated MAb 5H9 specific for prok [ 120 ]. In a more recent study, Jiang et al (2020) conducted a study to identify two monoclonal antibodies specific for mammalian skeletal troponins (sTn) (mAbs 6G1 and 8F10) and used them to develop a mAb-based sandwich enzyme-linked immunosorbent assay (ELISA) for mammalian meat detection [ 121 ]. In general test kits for the detection of meat species can be divided into two groups: ELISA tests and lateral flow devices.…”

Section: Detection Methods For Authentication and Adulteration Of Hal...mentioning

confidence: 99%

Adulteration detection technologies used for halal/kosher food products: an overview

2022

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In the Islamic and Jewish religions, there are various restrictions that should be followed in order for food products to be acceptable. Some food items like pork or dog meat are banned to be consumed by the followers of the mentioned religions. However, illegally, some food producers in various countries use either the meat or the fat of the banned animals during food production without being mentioned in the label on the final products, and this considers as food adulteration. Nowadays, halal or kosher labeled food products have a high economic value, therefore deceiving the consumers by producing adulterated food is an illegal business that could make large gains. On the other hand, there is an insistent need from the consumers for getting reliable products that comply with their conditions. One of the main challenges is that the detection of food adulteration and the presence of any of the banned ingredients is usually unnoticeable and cannot be determined by the naked eye. As a result, scientists strove to develop very sensitive and precise analytical techniques. The most widely utilized techniques for the detection and determination of halal/kosher food adulterations can be listed as High-Pressure Liquid Chromatography (HPLC), Capillary Electrophoresis (CE), Gas Chromatography (GC), Electronic Nose (EN), Polymerase Chain Reaction (PCR), Enzyme-linked Immuno Sorbent Assay (ELISA), Differential Scanning Calorimetry (DSC), Nuclear Magnetic Resonance (NMR), Near-infrared (NIR) Spectroscopy, Laser-induced Breakdown Spectroscopy (LIBS), Fluorescent Light Spectroscopy, Fourier Transform Infrared (FTIR) Spectroscopy and Raman Spectroscopy (RS). All of the above-mentioned techniques were evaluated in terms of their detection capabilities, equipment and analysis costs, accuracy, mobility, and needed sample volume. As a result, the main purposes of the present review are to identify the most often used detection approaches and to get a better knowledge of the existing halal/kosher detection methods from a literature perspective.

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Section: Detection Methods For Authentication and Adulteration Of Hal...mentioning

confidence: 99%

Adulteration detection technologies used for halal/kosher food products: an overview

2022

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“…Protein-based detection methods, such as the enzyme-linked immunosorbent assay 10 and immunosensors, 11 have been developed for food authenticity. However, high temperatures and other conditions will lead to the denaturation of target proteins.…”

Section: ■ Introductionmentioning

confidence: 99%

CRISPR-Based Colorimetric Nucleic Acid Tests for Visual Readout of DNA Barcode for Food Authenticity

Yin

Yang

Yongzhe

et al. 2022

J. Agric. Food Chem.

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Food authenticity is a critical issue associated with the economy, religion, and food safety. Herein, we report a label-free and colorimetric nucleic acid assay for detecting DNA barcodes, enabling the determination of food authenticity with the naked eye. This method, termed the CRISPR-based colorimetric DNA barcoding (Cricba) assay, utilizes CRISPR/Cas12a (CRISPR = clustered regularly interspaced short palindromic repeats; Cas = CRISPR associated protein) to specifically recognize the polymerase chain reaction (PCR) products for further trans-cleavaging the peroxidase-mimicking G-quadruplex DNAzyme. Based on this principle, the presence of the cytochrome oxidase subunit I gene could be directly observed with the naked eye via the color change of 3,3′,5,5′-tetramethylbenzidine sulfate (TMB). The whole detection process, including PCR amplification and TMB colorimetric analysis, can be completed within 90 min. The proposed assay can detect pufferfish concentrations diluted to 0.1% (w/w) in a raw pufferfish mixture, making it one of the most sensitive methods for food authenticity. The robustness of the assay was verified by testing four common species of pufferfish, including Lagocephalus inermis, Lagocephalus spadiceus, Takifugu bimaculatus, and Takifugu alboplumbeus. The assay is advantageous in easy signal readout, high sensitivity, and general applicability and thus could be a competitive candidate for food authenticity.

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“…Numerous analytical techniques using various instrumental methods have been developed for meat products authentication, such as gas chromatography-flame ionization detector (GC-FID) [ 7 ], gas chromatography-mass spectrometry (GC-MS) [ 8 ], high-performance liquid chromatography with UV-Vis and fluorescence detector, liquid chromatography-mass spectrometry (LC-MS) [ 9 , 10 ], vibrational spectroscopy such as Fourier transform infrared (FTIR) spectroscopy [ 11 ], near-infrared (NIR) spectroscopy [ 12 ], Raman spectroscopy [ 13 ], DNA-based methods such as polymerase chain reaction (PCR) and real time-polymerase chain reaction (RT-PCR) as well as protein-based methods using enzyme-linked immunosorbent assay (ELISA) [ 14 , 15 ]. Methods based on RT-PCR have been widely used in most countries for halal authentication of meat products, such as the detection of pork in beef meatballs [ 3 ], analysis of wild boar in beef meatballs [ 16 ], and detection of dog meat in beef meatballs [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Pork in Beef Meatballs Using LC-HRMS Based Untargeted Metabolomics and Chemometrics for Halal Authentication

et al. 2022

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Adulteration of high-quality meat products using lower-priced meats, such as pork, is a crucial issue that could harm consumers. The consumption of pork is strictly forbidden in certain religions, such as Islam and Judaism. Therefore, the objective of this research was to develop untargeted metabolomics using liquid chromatography-high resolution mass spectrometry (LC-HRMS) combined with chemometrics for analysis of pork in beef meatballs for halal authentication. We investigated the use of non-targeted LC-HRMS as a method to detect such food adulteration. As a proof of concept using six technical replicates of pooled samples from beef and pork meat, we could show that metabolomics using LC-HRMS could be used for high-throughput screening of metabolites in meatballs made from beef and pork. Chemometrics of principal component analysis (PCA) was successfully used to differentiate beef meatballs and pork meatball samples. Partial least square-discriminant analysis (PLS-DA) clearly discriminated between halal and non-halal beef meatball samples with 100% accuracy. Orthogonal projection to latent structures-discriminant analysis (OPLS-DA) perfectly discriminated and classified meatballs made from beef, pork, and a mixture of beef-pork with a good level of fitness (R2X = 0.88, R2Y = 0.71) and good predictivity (Q2 = 0.55). Partial least square (PLS) and orthogonal PLS (OPLS) were successfully applied to predict the concentration of pork present in beef meatballs with high accuracy (R2 = 0.99) and high precision. Thirty-five potential metabolite markers were identified through VIP (variable important for projections) analysis. Metabolites of 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine, acetyl-l-carnitine, dl-carnitine, anserine, hypoxanthine, linoleic acid, and prolylleucine had important roles for predicting pork in beef meatballs through S-line plot analysis. It can be concluded that a combination of untargeted metabolomics using LC-HRMS and chemometrics is promising to be developed as a standard analytical method for halal authentication of highly processed meat products.

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Monoclonal antibody-based sandwich ELISA for the detection of mammalian meats

Cited by 22 publications

References 29 publications

Adulteration detection technologies used for halal/kosher food products: an overview

Adulteration detection technologies used for halal/kosher food products: an overview

CRISPR-Based Colorimetric Nucleic Acid Tests for Visual Readout of DNA Barcode for Food Authenticity

Detection of Pork in Beef Meatballs Using LC-HRMS Based Untargeted Metabolomics and Chemometrics for Halal Authentication

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