Monoclonal antibody-based diagnostic assays for pepper mild mottle virus

Phatsaman, Teewasit; Hongprayoon, Ratchanee; Wasee, Sirikul

doi:10.1007/s42161-019-00421-4

Cited by 5 publications

(6 citation statements)

References 10 publications

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“…As PMMoVs, they were inducing mild mottle in plants sampled in the field. Serological tests with two anti-PMMoV IgGs showed cross-reaction among them, similar to those obtained in other works [45, 46]. In fact, these three viruses were genetically close in the phylogenetic tree made from tobamovirus genome sequences available on public databases.…”

Section: Full-textsupporting

confidence: 87%

Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles

Vélez‐Olmedo

Fribourg

Melo

et al. 2021

Journal of General Virology

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Tobamoviruses are often referred to as the most notorious viral pathogens of pepper crops. These viruses are not transmitted by invertebrate vectors, but rather by physical contact and seeds. In this study, pepper plants displaying mild mottle and mosaic symptoms were sampled in four different regions of Peru. Upon double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) tests, seven samples cross-reacted weakly with antibodies against pepper mild mottle virus (PMMoV), suggesting the presence of tobamoviruses. When employing RT-PCR, conserved primers amplified cDNA fragments of viruses from two putative new tobamovirus species in the samples. The complete genome of two representative isolates were, therefore, sequenced and analysed in silico. These viruses, which were tentatively named yellow pepper mild mottle virus (YPMMoV) and chilli pepper mild mottle virus (CPMMoV), shared highest nucleotide genome sequence identities of 83 and 85 % with bell pepper mottle virus (BpeMV), respectively. Mechanical inoculation of indicator plants with YPMMoV and CPMMoV isolates did not show any obvious differences in host ranges. These viruses were also inoculated mechanically on pepper plants harbouring different resistance L alleles to determine their pathotypes. Pepper plants carrying unfunctional L alleles (L 0) to tobamoviruses were infected by all isolates and presented differential symptomatology for YPMMoV and CPMMoV. On the other hand, pepper plants carrying L 1, L 2, L 3 and L 4 alleles were resistant to all isolates, indicating that these viruses belong to pathotype P0.

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Section: Full-textsupporting

confidence: 87%

Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles

Vélez‐Olmedo

Fribourg

Melo

et al. 2021

Journal of General Virology

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“…Polyclonal antibodies were also developed noncommercially for the detection of Tobamoviruses. The antibodies developed for Pepper mild mottle virus (PMMoV) specifically detected another four Tobamoviruses (including ToMV) in an antibody dilution of 1:1000 and infected plant sap dilution of 1:5120 [48]. The polyclonal antibody developed for the detection of ToMV exhibited twice as high sensitivity in ELISA testing as commercial antibodies [49].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Mrkvová

Hančinský

Grešíková

et al. 2022

Viruses

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Plant viruses threaten agricultural production by reducing the yield, quality, and economical benefits. Tomato mosaic virus (ToMV) from the genus Tobamovirus causes serious losses in the quantity and quality of tomato production. The management of plant protection is very difficult, mainly due to the vector-less transmission of ToMV. Resistant breeding generally has low effectiveness. The most practical approach is the use of a rapid diagnostic assay of the virus’ presence before the symptoms occur in plants, followed by the eradication of virus-infected plants. Such approaches also include serological detection methods (ELISA and Western immunoblotting), where antibodies need to be developed for an immunochemical reaction. The development and characterization of polyclonal antibodies for the detection of ToMV with appropriate parameters (sensitivity, specificity, and cross-reactivity) were the subjects of this study. A new polyclonal antibody, AB-1, was developed in immunized rabbits using the modified oligopeptides with antigenic potential (sequences are revealed) derived from the coat protein of ToMV SL-1. the developed polyclonal antibody. AB-1, showed higher sensitivity when compared with commercially available analogs. It also detected ToMV in infected pepper and eggplant plants, and detected another two tobamoviruses (TMV and PMMoV) and ToMV in soil rhizosphere samples and root residues, even two years after the cultivation of the infected tomato plant.

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“…ELISA is used not only in human diagnostics but also in the agricultural sector, particularly for the detection of certain plant viruses, fungal phytopathogens, and viral pathogens. Usually, the assay detects proteins (e.g., a viral coat protein) specific to an individual genus or species via polyclonal or monoclonal antibodies [26]. The detection of these proteins is accomplished by complexing antibodies and antigens to produce measurable, usually colored results.…”

Section: Serological Testsmentioning

confidence: 99%

Applicability of Smart Tools in Vegetable Disease Diagnostics

Ovesná

Tsiropoulos²,

Collier

et al. 2023

Agronomy

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Various diseases and pests cause serious damage to vegetable crops during the growing season and after harvesting. Growers attempt to minimize losses by protecting their crops, starting with seed and seedling treatments and followed by monitoring their stands. In many cases, synthetic pesticide treatments are applied. Integrated pest management is currently being employed to minimize the impact of pesticides upon human health and the environment. Over the last few years, “smart” approaches have been developed and adopted in practice to predict, detect, and quantify phytopathogen occurrence and contamination. Our review assesses the currently available ready-to-use tools and methodologies that operate via visual estimation, the detection of proteins and DNA/RNA sequences, and the utilization of brand-new innovative approaches, highlighting the availability of solutions that can be used by growers during the process of diagnosing pathogens.

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Monoclonal antibody-based diagnostic assays for pepper mild mottle virus

Cited by 5 publications

References 10 publications

Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles

Tobamoviruses of two new species trigger resistance in pepper plants harbouring functional L alleles

Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Applicability of Smart Tools in Vegetable Disease Diagnostics

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