Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase‐M in developing and adult rat lung

Fujiwara, Naoyuki; Ikeda, Mitsunobu; Hirabayashi, Susumu; Mori, Hiroki; Shirasawa, Madoka; Kansaku, Ai; Sunamori, Makoto; Hata, Yoshinobu

doi:10.1111/j.1440-1843.2006.00961.x

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…CpM expression has been described in the developing and adult lung in Alveolar type I cells [21,22]. Recently, the expression of CpM was described to be one of the genes expressed in the Foxa2-expressing endoderm cells [23].…”

Section: Discussionmentioning

confidence: 99%

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells

et al. 2011

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BackgroundWe developed an efficient in vitro method to differentiate mouse ES cells into the definitive endoderm (DE) and then Pdx1-expressing pancreatic lineages using mesodermal-derived supporting cells, M15. Using this method, resulting ES cell-derived DE and Pdx1-expressing cells were isolated by cell sorting, and their gene expression profiles were investigated with DNA microarray. Genes that were specifically expressed in DE and/or in Pdx1-expressing cells were extracted and their expression patterns in normal embryonic development were studied.ResultsGenes whose expression increased in DE and Pdx1 positive cells compared to the undifferentiated ES cells were chosen and in situ hybridizations were performed. Out of 54 genes examined, 27 were expressed in the DE of E8.5 mouse embryos and 15 genes were expressed in distinct domains in the pancreatic buds of E14.5 embryos. Among those genes expressed were Foxq1, CpM, Foxp4, Pcdh1, and Zmiz1, which were previously reported in other endodermal tissues. Genes, such as Parm1, Tmem184a, Hipk2 and Sox4 were reported to be expressed during early pancreatic development. Nptx2, C2cd4b, Tcf7l2 and Kiss1r were reported to be associated with beta cell or pancreatic functions in the adult. Akr1c19, Aebp2, Pbxip1 and Creb3l1, were novel and have not been described as being expressed either in DE or the pancreas.ConclusionsWe identified 27 genes, including 4 novel genes expressed in DE and pancreatic progenitor cells during normal development using an ES cell in vitro differentiation system. These results showed that DE cells and Pdx1/GFP-expressing cells obtained from our M15 based differentiation method mimic cells during the normal developmental processes. Additionally, ES cells are an excellent model for studies of early developmental processes.

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Section: Discussionmentioning

confidence: 99%

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells

et al. 2011

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“…Among the genes whose expression is increased in the ES cell-derived DE population, Foxq1 [50], CpM [51][52][53], Foxp4 [54,55], Pcdh1 [56], and Zmiz1 [57] were found to be expressed in the foregut, hindgut, or whole gut at E8.5. Parm1 [6], Tmem184 [58], HIPK-2 [59], Nptx2 [60,61], Tcf7l2 [62][63][64][65], C2Cd4b [66], Sox4 [67][68][69], and Kiss1r [70][71][72] were revealed for the first time to be expressed at this early stage of E8.5 and E14.5.…”

Section: Daf1/cd55 Foxq1 Nptx2 and Pga5mentioning

confidence: 99%

Profiling of Embryonic Stem Cell Differentiation

2014

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■ AbstractEmbryonic stem (ES) cells have been shown to recapitulate normal developmental stages. They are therefore a highly useful tool in the study of developmental biology. Profiling of ES cell-derived cells has yielded important information about the characteristics of differentiated cells, and allowed the identification of novel marker genes and pathways of differentiation. In this review, we focus on recent results from profiling studies of mouse embryos, human islets, and human ES cell-derived differentiated cells from several research groups. Global gene expression data from mouse embryos have been used to identify novel genes or pathways involved in the developmental process, and to search for transcription factors that regulate direct reprogramming. We introduce gene expression databases of human pancreas cells (Beta Cell Gene Atlas, EuroDia database), and summarize profiling studies of islet-or human ES cell-derived pancreatic cells, with a focus on gene expression, microRNAs, epigenetics, and protein expression. Then, we describe our gene expression profile analyses and our search for novel endoderm, or pancreatic, progenitor marker genes. We differentiated mouse ES cells into mesendoderm, definitive endoderm (DE), mesoderm, ectoderm, and Pdx1-expressing pancreatic lineages, and performed DNA microarray analyses. Genes specifically expressed in DE, and/or in Pdx1-expressing cells, were extracted and their expression patterns in normal embryonic development were studied by in situ hybridization. Out of 54 genes examined, 27 were expressed in the DE of E8.5 mouse embryos, and 15 genes were expressed in distinct domains in the pancreatic buds of E14.5 mouse embryos. Akr1c19, Aebp2, Pbxip1, and Creb3l1 were all novel, and none has been described as being expressed, either in the DE, or in the pancreas. By introducing the profiling results of ES cell-derived cells, the benefits of using ES cells to study early embryonic development will be discussed.

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“…The high CPM activity may stem from its induction and release in acute lung disease (16). Importantly, CPM is associated with monocyte to macrophage differentiation and regarded as a marker of macrophage maturation, indicating it may be important in inflammatory responses (18,19). Correlation analysis results indicate that rs73329476 may be associated with the expression of CPM, subjects carrying the risk CT and TT genotypes at rs73329476 had lower mRNA expression level of CPM as compared with those carrying CC genotype (P ¼ 0.0252) ( Fig.…”

Section: Discussionmentioning

confidence: 99%

A genome-wide association study identifies susceptibility loci of silica-related pneumoconiosis in Han Chinese

Chu¹,

Chen

et al. 2014

Human Molecular Genetics

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Pneumoconiosis is the most serious occupational disease in China and its leading cause is occupational silica exposure. Pneumoconiosis takes several years to develop depending on the exposure level of silica. However, individual variation in the susceptibility to pneumoconiosis has been observed among the subjects with similar exposure. We conducted a genome-wide screening with 710,999 single nucleotide polymorphisms (SNPs) in a cohort of 400 coal workers (202 cases and 198 exposed controls) for pneumoconiosis susceptible loci. Seven promising variants were evaluated in an independent cohort of 568 coal workers (323 cases and 245 exposed controls), followed by a second replication on 463 iron ore workers (167 cases and 296 exposed controls). By pooling all of the genome-wide association studies and replication stages together, we found a genome-wide significant (P < 5.0 × 10(-8)) association for rs73329476 (P = 1.74 × 10(-8), OR = 2.17, 95% CI = 1.66-2.85) and two additional replicated associations for rs4320486 (P < 0.05) and rs117626015 (P < 0.05) with combined P-values of 4.29 × 10(-6) and 5.05 × 10(-6), respectively. In addition, the risk allele T of rs73329476 was significantly associated with lower mRNA expression levels of carboxypeptidase M (CPM) in total cellular RNA from whole blood of 156 healthy individuals (P = 0.0252). The identified pneumoconiosis susceptibility loci may provide new insights into the pathogenesis of pneumoconiosis, and may also have some clinical utility for risk prediction for pneumoconiosis and high-risk population screening for workers with occupational silica exposure.

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Monoclonal antibody 7F9 recognizes rat protein homologous to human carboxypeptidase‐M in developing and adult rat lung

Cited by 5 publications

References 15 publications

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells

An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells

Profiling of Embryonic Stem Cell Differentiation

A genome-wide association study identifies susceptibility loci of silica-related pneumoconiosis in Han Chinese

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