Monoclonal antibody 2C5: A marker for a subpopulation of small neurones in rat dorsal root ganglia

Lawson, Sally N.; Harper, Ei; Harper, A. A.; Garson, Jeremy A.; Coakham, H. B.; Randle, B.J.

doi:10.1016/0306-4522(85)90009-0

Cited by 39 publications

(10 citation statements)

References 24 publications

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“…Subpopulations of neurones containing specific carbohydrate epitopes which can be labelled with the monoclonal antibodies 2C5 and LA4 (Dodd & Jessel, 1985;Lawson, Harper, Harper, Garson, Coakham & Randle, 1985) were also reduced by 51 and 53 % respectively. The number of neurones containing FRAP was decreased by 37 %.…”

Section: Peptide Histochemistry Of Injured Primary Sensory Neuronesmentioning

confidence: 97%

Pathobiological reactions of C‐fibre primary sensory neurones to peripheral nerve injury

Jancsó

1992

Experimental Physiology

101

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Mammalian primary sensory neurones display profound anatomical and chemical changes in response to injury of their peripheral processes. Peripheral nerve damage results in transganglionic degeneration of central terminals of A‐fibre primary afferent neurones terminating in the deeper layers of the medullary or spinal dorsal horn. The depletion of neuropeptides and other neurone‐specific macromolecules from neurones in sensory ganglia and from the superficial dorsal horn, the known central projection area of C‐fibre primary afferents, is a salient phenomenon commencing after peripheral nerve lesions. A recently devised new experimental procedure, termed the capsaicin‐gap method, permitted evaluation of the transganglionic degenerative phenomena which develop in C‐fibre primary sensory neurones after a lesion is inflicted upon their peripheral branches. The experimental findings indicated that C‐fibre primary afferent terminals underwent transganglionic degeneration following a perineural treatment with capsaicin, and that this was associated with, and probably resulted from, ganglionic cell degeneration. Studies on the effects of peripheral nerve section yielded similar results. It has therefore been suggested that the observed depletion of specific macromolecules, including sensory peptides, specific glycoconjugates and sensory neurone specific acid phosphatase, may be accounted for, at least in part, by an irreversible loss of sensory ganglion neurones. In contrast, many injured neurones express peptides, including vasoactive intestinal polypeptide, peptide histidine‐isoleucine and galanin, which can be demonstrated in only a few neurones under normal conditions. These seem to be involved in dorsal horn regenerative and/or compensatory processes following peripheral nerve damage. There is suggestive evidence that the partial deafferentation caused by the transganglionic degeneration of C‐fibre primary afferents creates favourable circumstances for an anatomical rearrangement of neuronal connections within the spinal cord dorsal horn. These changes may provide a morphological substrate of some of the functional alterations demonstrated after peripheral nerve lesions.

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Section: Peptide Histochemistry Of Injured Primary Sensory Neuronesmentioning

confidence: 97%

Pathobiological reactions of C‐fibre primary sensory neurones to peripheral nerve injury

Jancsó

1992

Experimental Physiology

101

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“…Globoseries carbohydrates, and the GM1 ganglioside recognized by the cholera toxin B subunit, are associated with larger diameter, rapid conducting, low threshold afferents which terminate in the deeper laminar of the dorsal horn (Dodd, et al, 1984;Robertson, et al, 1991). In contrast, the small diameter, slowly conducting afterents which terminate in the superficial dorsal horn are associated with various lactoseries and ganglioseries carbohydrates, a subset of which terminates with an o~-D-Galactose (RGal) residue Lawson et al, 1985;Alvarez et al, 1989a;Chou et al, 1989). * To whom correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 94%

Transganglionic labelling of primary sensory afferents in the rat lumbar spinal cord: comparison between wheatgerm agglutinin and the I-B4 isolectin fromBandeiraea simplicifolia

et al. 1994

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We recently reported that the I-B4 isolectin from Bandeiraea simplicifolia could be used as a transganglionic neuronal tracer which appears to be selective for unmyelinated cutaneous afferents (C fibres) and their terminals in the superficial dorsal horn. As terminals in the superficial dorsal horn are also labelled by wheatgerm agglutinin, we sought to compare these two neuronal tracers. Three days after the injection of 1% wheatgerm agglutinin-HRP or 1% BSI-B4-HRP into the sciatic nerve of adult rats the lumbar spinal cord was processed for HRP reactivity. The majority of labelled structures was found in the superficial dorsal horn, with fewer labelled structures seen in the overlying white matter (including Lissauer's tract). In wheatgerm agglutinin-HRP experiments most labelled structures were synaptic terminals (63%) and unmyelinated axons (32%). About 3% of wheatgerm agglutinin-HRP-labelled structures were fine myelinated fibres (which were found only in lamina I and outer lamina II) and about 2% of label was located in neuronal somata. In contrast, label from BSI-B4-HRP experiments was found only in synaptic terminals (37%) and unmyelinated axons (63%). Previous studies have shown that small diameter dorsal root ganglion neurons and their terminals in the superficial dorsal horn express a range of structurally related carbohydrates that contain binding sites for BSI-B4 or wheatgerm agglutinin or both. Comparison of the labelling patterns produced by the two transganglionic tracers in the present study suggests that unmyelinated sciatic afferents express wheatgerm agglutinin and BSI-B4 binding sites, but some thin myelinated afferents, and a distinct form of synaptic terminal in lamina I/II outer, express the wheatgerm agglutinin binding site and not the BSI-B4 binding site.

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“…On the basis of cytologic criteria at the light and electron microscopic level, three major classes of DRG neurons have been described: type A or large light-colored cells, type B or intermediate-sized dark-colored cells, and type C or small-sized dark-colored cells ( Andres , 196 1 ;Lawson, 1961;Rambourg et al, 1983;Sommer et al, 1985). More recently, monoclonal antibodies have been produced that specifically label large cells (Lawson et al, 1984), small cells (Lawson et al, 1985), or subsets of small and intermediate neurons (Dodd et al, 1984). However, the latter populations of DRG neurons have been characterized mainly on the basis of their cytochemical content of peptides, neurotransmitters, or neurotransmitter-related enzymes.…”

Section: Introductionmentioning

confidence: 99%

Neurotransmitter phenotype plasticity in cultured dissociated adult rat dorsal root ganglia: An immunocytochemical study

Schoenen

Delrée

Leprince

et al. 1989

J of Neuroscience Research

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Culturing sympathetic ganglion neurons in vitro may modify phenotypic expression of some neurotransmitters. For dorsal root ganglia (DRG), contradictory results have been reported; most studies have used immature material. We have therefore performed a detailed immunocytochemical analysis of the transmitter content of cultured adult rat DRG neurons. To demonstrate possible modifications of neurotransmitter phenotypes, we have compared the results obtained with the same techniques on neurons cultured for 3 days and on freshly dissociated DRG cells. Also, the transmitter profile of cultured neurons was compared with that known from in situ studies. Out of 22 antigens studied, 20 were detected in cultured DRG neurons. All of them were expressed in small and/or intermediate-sized cells. Large neurons only contained CGRP, VIP, NPY, beta-END, ENK, and GABA. The percentage of immunostained neurons varied for the various antisera: less than 10% of cultured neurons were positive for ENK, beta-LPH, beta-END, DYN, VASO, and OXY; 10-30% for SOM, CCK, CAT, and SP; and greater than 30% for NPY, CRF, GLU, NT, VIP, GABA, GRP, CGRP, 5-HT, and TRH. In the latter two groups of transmitters (except CGRP), the proportion of immunoreactive neurons was by far larger in cultured than in freshly dissociated DRG. The most pronounced (greater than 25%) increase in the proportion of positively stained neurons after culturing was observed for the GRP, CRF, TRH, and 5-HT antisera. Serotonin was the only transmitter identified in cultured but not in freshly dissociated cells. These data indicate, on one hand, that various antigens, for example, CAT, GABA, NT, TRH, NPY, beta-LPH, and beta-END, which up to now have not been described in DRG in situ, can be detected immunocytochemically a few hours after dissociation of adult rat DRG. On the other hand, several transmitters, for example, VIP, NPY, SP, GABA, GLU, NT, GRP, CRF, TRH, and 5-HT, are expressed in a significantly higher proportion of cells in cultured than in freshly dissociated preparations. This might reflect a change in the phenotypic expression of transmitters due to the new environment generated by the culture conditions, a hypothesis that can be tested by measuring specific mRNA levels. Moreover, considering the plasticity and multipotentiality of their transmitter phenotype, cultured adult DRG neurons might represent an interesting material for autografts into the injured central nervous system.

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Monoclonal antibody 2C5: A marker for a subpopulation of small neurones in rat dorsal root ganglia

Cited by 39 publications

References 24 publications

Pathobiological reactions of C‐fibre primary sensory neurones to peripheral nerve injury

Pathobiological reactions of C‐fibre primary sensory neurones to peripheral nerve injury

Transganglionic labelling of primary sensory afferents in the rat lumbar spinal cord: comparison between wheatgerm agglutinin and the I-B4 isolectin fromBandeiraea simplicifolia

Neurotransmitter phenotype plasticity in cultured dissociated adult rat dorsal root ganglia: An immunocytochemical study

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