Monoclonal Antibodies to the Recombinant Protein TmpA of the<i>Treponema pallidum</i>

Moreno, Adys I. Brito; Bas, Carmen Acosta; Rodríguez, Maya; Conde, Ileana B. Baluja; Carballo, Sadys Feal; Martínez, Luisa

doi:10.1089/153685903771797101

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…At present, the antigen screening targets for syphilis diagnosis are Tp membrane lipoprotein ( 14 , 15 , 23 , 24 ) or flagellin ( 25 ); however, they have certain limitations. The number and expression rate of syphilis-specific plasma membrane lipoproteins is low.…”

Section: Discussionmentioning

confidence: 99%

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Serological evaluation of antigen Tp0693 for diagnosis of syphilis

et al. 2017

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The aim of the present study was to evaluate the diagnostic value of the Treponema pallidum (Tp) antigen Tp0693 for syphilis. ELISA was used to examine the serum levels of Tp0693. The sample-to-cutoff ratio (S/CO) value was used to generate a receiver operating characteristic (ROC) curve. A correlation analysis was performed to compare the detection efficacy of Tp0693-specific ELISA, Treponema pallidum Particle Agglutination (TPPA), Tolulized Red Unheated Serum test (TRUST) and LiZhu™ Tp-ELISA. The area under the ROC curve was 0.99, indicating good diagnostic efficacy. When the diagnostic specificity reached 100%, the diagnostic sensitivity was up to 93.5%. Tp0693-specific ELISA results were not correlated with those of TPPA, TRUST and LiZhu™ Tp-ELISA (correlation coefficient, 0.122, 0.114 and 0.025, respectively). The latent syphilis rate was highest (12%, 9/75) for all syphilis specimens with a S/CO in the grey area. In conclusion, for syphilis specimens with a S/CO in the grey area, TPPA should be used for further confirmation of the diagnosis. Tp0693 may be used as a diagnostic antigen for syphilis; however, further study regarding its potential use is required.

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Section: Discussionmentioning

confidence: 99%

“…The syphilis-specific recombinant diagnostic antigens in Tp antibody detection kits are predominantly membrane lipoproteins such as chimera of Tp17, Tp47, Tp15 and treponemal membrane protein A ( 14 , 15 ). Detection of syphilis using these antigens has certain disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Serological evaluation of antigen Tp0693 for diagnosis of syphilis

et al. 2017

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“…The identification and characterization of new immunodominant antigens of T. pallidum is central to the improvement of the current laboratory diagnosis of syphilis, a disease that has resurged worldwide in recent years, including in China. [19][20][21] A variety of T. pallidum proteins, including TpN44.5, TpN15, TpN17, TpN47, Tp0453, Tp92 (Tp0326), and TpF1 (Tp1038), 5,6,[22][23][24] have been tested in recent decades. Some of these antigens have been used in commercial tests that exhibit high sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

“…4 In recent years, automated enzyme immunoassays (EIAs) and chemiluminescence immunoassays (CIAs) have also been developed for the serodiagnosis of syphilis. These assays, using one or more of the recombinant T. pallidum proteins TpN15 (Tp0171), TpN17 (Tp0435), TpN44.5 (TmpA, Tp0768), and TpN47 (Tp0574), 5,6 have gained the interest of researchers in the field of syphilis diagnosis because they are objective, reproducible, automated, and computerized. 7 More importantly, these tests have led some large laboratories in the USA to screen patients with the reverse algorithm, which begins with a treponemal test; reactive tests are followed by a quantitative non-treponemal test and discordant samples must be rescreened with a second and different treponemal test.…”

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confidence: 99%

A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis

Xie

Jiang

et al. 2016

International Journal of Infectious Diseases

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“…Several T. pallidum proteins have been tested, including TpN15 (Tp0171), TpN17 (Tp0435), TpN44.5 (TmpA, Tp0768), TpN47 (Tp0574), Tp0453, Tp92 (Tp0326), and Tp0965 (13)(14)(15)(16)(17). Although these recombinant antigens are sometimes used in combination in commercial tests and exhibit high sensitivity, not all of these antigens can be used for the detection of the early stage of syphilis.…”

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confidence: 99%

Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis

Jiang¹,

Zhao²,

Xiao³

et al. 2013

Clin. Vaccine Immunol.

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Syphilis is a chronic infection caused by Treponema pallidum subsp. pallidum, and diagnosis with sensitive and specific methods is a challenging process that is important for its prevention and treatment. In the present study, we established a recombinant protein TpF1-based indirect immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) and a Western blot assay for human and rabbit sera. The 20-kDa recombinant protein TpF1 was detected by Western blotting performed with sera from rabbits immunized with recombinant TpF1 and infected with the T. pallidum Nichols strain and T. pallidum clinical isolates but was not detected by Western blotting with sera from uninfected rabbits. The sensitivity of the recombinant protein was determined by screening sera from individuals with primary, secondary, latent, and congenital syphilis (n ‫؍‬ 82). The specificity of the recombinant protein was determined by screening sera from uninfected controls (n ‫؍‬ 30) and individuals with potentially cross-reactive infections, including Lyme disease (n ‫؍‬ 30) and leptospirosis (n ‫؍‬ 5). The sensitivities of TpF1-based ELISAs were 93.3%, 100%, 100%, and 100% for primary, secondary, latent, and congenital syphilis, respectively, and the specificities were all 100% for sera from uninfected controls and individuals with potentially cross-reactive infections. In Western blot assays, the sensitivities and specificities of TpF1 for human sera were all 100%. The reactivities of TpF1 with syphilitic sera were proportional to the titers of the T. pallidum particle agglutination (TPPA) assay. These data indicate that the recombinant protein TpF1 is a highly immunogenic protein in human and rabbit infections and a promising marker for the screening of syphilis.

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Monoclonal Antibodies to the Recombinant Protein TmpA of theTreponema pallidum

Cited by 5 publications

References 9 publications

Serological evaluation of antigen Tp0693 for diagnosis of syphilis

Serological evaluation of antigen Tp0693 for diagnosis of syphilis

A novel ELISA using a recombinant outer membrane protein, rTp0663, as the antigen for serological diagnosis of syphilis

Evaluation of the Recombinant Protein TpF1 of Treponema pallidum for Serodiagnosis of Syphilis

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