Monoclonal antibodies to snakehead, Channa striata immunoglobulins: Detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs

Sood, Neeraj; Chaudhary, Dharmendra Kumar; Rathore, Gaurav; Singh, Apekshita; Lakra, W.S.

doi:10.1016/j.fsi.2010.12.004

Cited by 16 publications

(20 citation statements)

References 26 publications

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“…In fish, B cells are readily detectable in the thymus (Vigliano et al, 2011;DeLuca et al, 1983;Miyadai et al, 2004;Sood et al, 2011Sood et al, , 2012 as well as in the kidney ( Figure 6E), the major hematopoietic site in teleosts; the proportion of B cells in the fish thymus can reach up to 5%. B cells remain in the fish thymus even in the absence of foxn1 ( Figure S5F), indicating that coexpression of foxn4 and foxn1 genes in TECs of the fish thymus supports B cell development alongside T cell development ( Figure 2).…”

Section: Formation Of a Fish-like Thymus In Micementioning

confidence: 99%

Conversion of the Thymus into a Bipotent Lymphoid Organ by Replacement of Foxn1 with Its Paralog, Foxn4

et al. 2014

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The thymus is a lymphoid organ unique to vertebrates, and it provides a unique microenvironment that facilitates the differentiation of immature hematopoietic precursors into mature T cells. We subjected the evolutionary trajectory of the thymic microenvironment to experimental analysis. A hypothetical primordial form of the thymus was established in mice by replacing FOXN1, the vertebrate-specific master regulator of thymic epithelial cell function, with its metazoan ancestor, FOXN4, thereby resetting the regulatory and coding changes that have occurred since the divergence of these two paralogs. FOXN4 exhibited substantial thymopoietic activity. Unexpectedly, histological changes and a functional imbalance between the lymphopoietic cytokine IL7 and the T cell specification factor DLL4 within the reconstructed thymus resulted in coincident but spatially segregated T and B cell development. Our results identify an evolutionary mechanism underlying the conversion of a general lymphopoietic organ to a site of exclusive T cell generation.

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Section: Formation Of a Fish-like Thymus In Micementioning

confidence: 99%

Conversion of the Thymus into a Bipotent Lymphoid Organ by Replacement of Foxn1 with Its Paralog, Foxn4

et al. 2014

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“…The Ig+ cells are not present in C. striatus thymus as observed in than other lymphoid organs including kidney and spleen of C. striatus (Sood et al . ).…”

Section: Ontology and Key Lymphoid Organs Of C Striatusmentioning

confidence: 97%

“…Recently, Sood et al . () have discovered that Ig+ cells are present in white and red pulp compartments of spleen. The concentration of Ig+ cells was observed to be higher in mantle layer of white pulp than the later.…”

Section: Ontology and Key Lymphoid Organs Of C Striatusmentioning

confidence: 99%

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Innate and adaptive immune molecules of striped murrel Channa striatus

Palanisamy

Bhatt

Kumaresan

et al. 2016

Reviews in Aquaculture

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Channa striatus, also called snakehead murrel, is an important freshwater teleost fish which has been widely cultured for its tasty flesh along with nutritional and medicinal values. The growth of both cultured and wild murrels is affected by various physical, chemical and biological factors. As a teleost fish, C. striatus is an intermediate organism between invertebrates and vertebrates. They have a well‐developed innate immune system than invertebrates and a primitive adaptive immune system compared to that of higher vertebrates, thus an interesting unique immune structure to explore. Studies have identified that a few external stimulants do instigate the immune system to fight against the pathogens at the time of infection in C. striatus. This review discusses the physicochemical and biological stress factors, immune system and immune molecules of C. striatus which are potentially involved in combating the stress factors.

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“…Polyclonal and monoclonal antibodies to fish Ig have been crucial for developing immunological tools to evaluate total and specific concentrations of serum antibodies produced in response to inoculated antigen and to monitor the occurrence and prevalence of pathogenic microorganisms (Crosbie & Nowak 2002, Beelen et al 2004, Grove et al 2006b, Li et al 2007, Rathore et al 2008, Suresh Babu et al 2008, Lim et al 2009, Santos et al 2009, Sood et al 2011, Boardman et al 2012, Hedfors et al 2012, Purcell et al 2012. Anti-immunoglobulin antibodies have also been used to characterize and study the ontogeny and tissue distribution of cells subsets bearing membrane bound Ig (Beelen et al 2004, Grove et al 2006a, Tian et al 2009 and in cross reactivity studies to gain information on phylogenetic relationship amongst different species (Swennes et al 2007, Rathore et al 2008, Bag et al 2009, Santos et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

Kreutz

Canova²,

Nied³

et al. 2016

Pesq. Vet. Bras.

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Knowledge on fish immunoglobulin (Ig) characteristics and the availability of monoclonal or polyclonal antibodies to fish Igs are essential to evaluate the humoral immune response and the Ig distribution on leukocyte cells. We demonstrated that silver catfish serum Ig is composed of one immunodominant H chain with approximately 75k Da and one L chain with approximately 28 kDa, similar to human IgM. Rabbit polyclonal antibodies to the catfish IgM-like Ig recognized both the H and L chain and were useful in developing an indirect ELISA to measure the production of antibodies in fish immunized with bovine serum albumin. Dot blot and western blot cross-reactivity studies indicated a wide degree of epitope sharing amongst Ig from several Siluriformes and Characiformes fish indigenous to Brazilian rivers. In these fish species, polyclonal antibodies reacted mostly with the H chain. The results presented here are central to the development of tools and strategies to investigate the antibody production to inoculated antigens and tissue distribution of Ig molecules in native fish species. Furthermore, because of the wide range of cross-reactivity, polyclonal antibodies to silver catfish IgM-like Ig might be used to develop immunoassays to measure the humoral immune response in other fish species.

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Monoclonal antibodies to snakehead, Channa striata immunoglobulins: Detection and quantification of immunoglobulin-positive cells in blood and lymphoid organs

Cited by 16 publications

References 26 publications

Conversion of the Thymus into a Bipotent Lymphoid Organ by Replacement of Foxn1 with Its Paralog, Foxn4

Conversion of the Thymus into a Bipotent Lymphoid Organ by Replacement of Foxn1 with Its Paralog, Foxn4

Innate and adaptive immune molecules of striped murrel Channa striatus

Characterization of an IgM-like immunoglobulin from silver catfish (Rhamdia quelen) serum and its use for the production of polyclonal antibodies and development of immunoassays

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