Monoclonal Antibodies to Six-Transmembrane Epithelial Antigen of the Prostate-1 Inhibit Intercellular Communication <i>In vitro</i> and Growth of Human Tumor Xenografts <i>In vivo</i>

Challita-Eid, Pia M.; Morrison, Kendall; Etessami, S.; An, Zili; Morrison, Karen; Pérez-Villar, Juan J.; Raitano, Arthur; Jia, Xiao‐Chi; Gudas, Jean M.; Kanner, Steven B.; Jakobovits, Aya

doi:10.1158/0008-5472.can-06-3849

Cited by 91 publications

(115 citation statements)

References 25 publications

Supporting

Mentioning

110

Contrasting

Order By: Relevance

“…Given that STEAP1 was shown to be expressed in prostate cancer metastases [3] and to be associated with invasive behaviour of Ewing tumors [34], an association between STEAP1 expression and tumor progression and patients' survival was investigated. A high STEAP1 expression was significantly associated with a reduced overall survival in patients with colorectal cancer (n=177; P = .02), with diffuse large B cell lymphoma (n=414; P = .008), with acute myeloid leukemia (n=79; P = .0005), or with multiple myeloma (MM) (n=206 P = .01 and n=345; P =.02) (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

“…Recent study described a function of STEAP1 in mediating the transfer of small molecules between adjacent cells in culture, indicating its potential role in tumor cell intercellular communication [3]. Antibodies directed to STEAP1 inhibited STEAP1 induced intercellular communication of prostate cancer cells in a dose-dependent manner.…”

Section: Normalized Steap1 Protein Expression Was Significantly Corrementioning

confidence: 99%

“…STEAP1 is highly expressed in human prostate cancer and is up-regulated in various cancers, including lung, bladder, colon, ovarian, and Ewing cancers [1,2,3]. Immunohistochemical analysis of clinical specimens demonstrates significant STEAP1 expression at the cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells [1].…”

Section: Introductionmentioning

confidence: 99%

“…Targeting STEAP1 appeared also useful for Tcell based immunotherapy in renal and bladder cancers [7]. Finally, STEAP1 was also described as an attractive target for antibody therapy in multiple solid tumors including prostate, renal and bladder cancers [3].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

STEAP1 is overexpressed in cancers: A promising therapeutic target

Moreaux

Kassambara

Hose

et al. 2012

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

The six-transmembrane epithelial antigen of prostate (STEAP) protein was identified in advanced prostate cancer and is up-regulated in multiple cancer cell lines, including prostate, bladder, colon, ovarian, and Ewing sarcoma. STEAP1 was described as a suitable antigen for T-cell-based or antibody-based immunotherapy.We have investigated the expression of STEAP1 in 40 human tumor types -brain, epithelial, lymphoid -and in their normal tissue counterparts using publicly available gene expression data, including the Oncomine Cancer Microarray database. STEAP1 was found significantly overexpressed in 11 cancers. In addition, high STEAP1 expression was associated with poor overall survival in colorectal cancer, diffuse large B cell lymphoma, acute myeloid leukemia and multiple myeloma.Taken together, these data suggest that STEAP1 is a potential therapeutic target for Tcell based immunotherapy or antibody therapy in a large panel of cancers.3

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Normalized Steap1 Protein Expression Was Significantly Corrementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

STEAP1 is overexpressed in cancers: A promising therapeutic target

Moreaux

Kassambara

Hose

et al. 2012

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…Because of its high tumor specificity and membrane-bound localization, STEAP1 might serve as a promising candidate for targeted therapy (6,10,11). In accordance, monoclonal antibodies against STEAP1 inhibit growth of xenografted prostate and bladder cancer (12).…”

Section: Introductionmentioning

confidence: 91%

STEAP1 Is Associated with the Invasive and Oxidative Stress Phenotype of Ewing Tumors

Grünewald

Diebold

Espósito

et al. 2012

Molecular Cancer Research

111

132

View full text Add to dashboard Cite

Ewing tumors comprise the second most common type of bone-associated cancer in children and are characterized by oncogenic EWS/FLI1 fusion proteins and early metastasis. Compelling evidence suggests that elevated levels of intracellular oxidative stress contribute to enhanced aggressiveness of numerous cancers, possibly including Ewing tumors. Using comprehensive microarray analyses and RNA interference, we identified the six-transmembrane epithelial antigen of the prostate 1 (STEAP1)-a membrane-bound mesenchymal stem cell marker of unknown function-as a highly expressed protein in Ewing tumors compared with benign tissues and show its regulation by EWS/FLI1. In addition, we show that STEAP1 knockdown reduces Ewing tumor proliferation, anchorage-independent colony formation as well as invasion in vitro and decreases growth and metastasis of Ewing tumor xenografts in vivo. Moreover, transcriptome and proteome analyses as well as functional studies revealed that STEAP1 expression correlates with oxidative stress responses and elevated levels of reactive oxygen species that in turn are able to regulate redox-sensitive and proinvasive genes. In synopsis, our data suggest that STEAP1 is associated with the invasive behavior and oxidative stress phenotype of Ewing tumors and point to a hitherto unanticipated oncogenic function of STEAP1. Mol Cancer Res; 10(1); 52-65. Ó2011 AACR.

show abstract

Six‐transmembrane epithelial antigen of the prostate 1 is associated with tumor invasion and migration in endometrial carcinomas

Sun

Xie

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Six-transmembrane epithelial antigen of the prostate 1 (STEAP1), a member of the STEAP family, is a general tumor antigen. However, no information has been available to date regarding the function of STEAP1 in the progression of endometrial carcinoma. In this study, we used in vitro and in vivo strategies to prove that STEAP1 plays an important role in the progression of endometrial carcinoma. Immunohistochemistry, immunocytochemistry, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and Western blot analysis were used to detect the expression of STEAP1 in normal endometrial cells and endometrial cancer cell lines. The progression of the cell cycle, plate clone formation assay, and transwell migration and invasion assays were performed to examine the effects of STEAP1 on cell proliferation, clonogenicity, migration, and their invasive capacity. In addition, we confirmed that STEAP1 was tightly correlated with the development of tumor in vivo. The relationship between epithelial to mesenchymal transition (EMT) and STEAP1 expression was evaluated by RT-qPCR and Western blot analysis. Matrix metalloproteinase (MMP) zymography assay was used to detect the activities of MMP2 and MMP9. STEAP1 was restrictively expressed in endometrial carcinoma and downregulation of the STEAP1 gene increased proliferation and clonogenicity, as well as promoted cell migration, invasion, and the progress of EMT. STEAP1 is downregulated in endometrial carcinoma and can restrict migration and invasion of endometrial carcinoma cells. Overall, STEAP1 may be an ideal target for tumor therapy and diagnosis in the future. K E Y W O R D S endometrial carcinoma, epithelial to mesenchymal transition, invasion, migration, six-transmembrane epithelial antigen of the prostate 1A standard streptavidin-biotin-peroxidase staining procedure was used, wherein formalin-fixed, paraffin-embedded endometrial tissues were cut into 5-μm slices. After a series of processing steps, such as dewaxing in xylene, rehydration, and antigen retrieval, sections were incubated with the mouse anti-human STEAP1 monoclonal antibody (ab117454; Abcam) at a dilution of 1﹕200 . Six-transmembrane epithelial antigen of the prostate 1 is associated with tumor invasion and migration in endometrial carcinomas.

show abstract

Monoclonal Antibodies to Six-Transmembrane Epithelial Antigen of the Prostate-1 Inhibit Intercellular Communication In vitro and Growth of Human Tumor Xenografts In vivo

Cited by 91 publications

References 25 publications

STEAP1 is overexpressed in cancers: A promising therapeutic target

STEAP1 is overexpressed in cancers: A promising therapeutic target

STEAP1 Is Associated with the Invasive and Oxidative Stress Phenotype of Ewing Tumors

Six‐transmembrane epithelial antigen of the prostate 1 is associated with tumor invasion and migration in endometrial carcinomas

Contact Info

Product

Resources

About