Monoclonal antibodies to phenobarbital-induced rat liver cytochrome P-450

Park, Sang S.; Fujino, Toyomi; Miller, Haruko; Guengerich, F. Peter; Gelboin, Harry V.

doi:10.1016/0006-2952(84)90576-8

Cited by 107 publications

(37 citation statements)

References 36 publications

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“…However, the antibody did not affect AE in microsomes from untreated rats. Similar results have previously been obtained for the effect of MAb-PB on A H H in microsomes from PR-treated and untreated animals [38].…”

Section: Effect O F ' M a H On Ahh Activity In Microsomes Ofrut Liversupporting

confidence: 88%

“…The effects of MAb-NBS (280 pg/ml of reaction mixture) o n the cell preparations were generally lower than those observed with microsomes (Fig. 2) or isolated cytochromes P-450 [38]. The maximal increase in monooxygenase activities in the presence of MAb-NBS did not exceed 36% of the controls.…”

Section: Efj'2ct Qf'mab-pb and Mah-mc On A H H Ecde And A E In Se mentioning

confidence: 65%

“…Steroids have been shown to induce a specific monooxygenase form which is capable of catalyzing aldrin epoxidation [50]. This isozyme was insensitive to the MAb-PB employed in this study as indicated by the lack of inhibition of AHH catalyzed by the purified isozyme from hepatic microsomes of pregnenolone-162-carbonitrile-treated rats [38]. As pointed out above dexamethasone causes a shift in the proportion of MAb-PB-sensitive AE relative to MAb-insensitive AE in C2Rev7 cells suggesting that the steroid may indeed induce a specific cytochrome P-450 form in the hepatoma cells, possi bly the form induced by pregnenolone-I 6a-carbonitrile iri vivo.…”

Section: Cj! T O Chuomes ' P-450'mentioning

confidence: 82%

“…The MAb-MC used is that described as 1-8-1 and belongs to a group of antibodies which inhibit by more than 90% the AHH and ECDE of a cytochrome P-450 purified from the liver of MC-treated rats. The characterization of the MAb has been presented previously by Park et al [37]. MAb-PB previously described as MAb-2-66-3p5 [38] inhibits by more than 90"/0 AHH and ECDE of a PB-induced rat liver cytochrome P-450 [38].…”

Section: Prcjparution Of M a Bmentioning

confidence: 99%

“…MAb-PB previously described as MAb-2-66-3p5 [38] inhibits by more than 90"/0 AHH and ECDE of a PB-induced rat liver cytochrome P-450 [38]. MAb-NBS was a control MAb produced by hybrid cells formed from spleen cells of unimmunized mice and myeloma cells [37].…”

Section: Prcjparution Of M a Bmentioning

confidence: 99%

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Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital

Wiebel¹,

Park

Kiefer³

et al. 1984

Eur J Biochem

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We have studied the expression of aldrin epoxidase (AE), 7-ethoxycoumarin-0-deethylase (ECDE), and aryl hydrocarbon (benzo[u]pyrene) hydroxylase (AHH) in nine differentiated or dedifferentiated cell lines derived from H4IIEC3 rat hepatoma cells. The nature of the cytochromes P-450 mediating AE, ECDE and AHH activities was analysed using monoclonal antibodies (MAb) made to the major 3-methylcholanthrene-induced cytochrome P-450 (MAb-MC) or phenobarbital-induced cytochrome P-450 (MAb-PB) from rat liver. The cells were treated with 5 pM dexamethasone for 30 h to increase the levels of the monooxygenase activities.(a) The six differentiated cell lines examined (Faza967, Fao, HFI-4, 2sFou, C2Rev7, and H4IIEC3/G contained MAb-PB-sensitive AE comprising 30-75% of the total AE activity. In most of these cell lines MAb-PB also markedly inhibited ECDE; however, the antibody had a considerably weaker effect on AHH. The results show that the hepatoma cells examined express to various degrees phenobarbital-inducible cytochrome P-450 and/or 3-methylcholanthrene-inducible cytochrome P-450. These cell lines are versatile tools for studying the regulation of monooxygenase activities and analysing their role in the activation and inactivation of xenobiotics such as carcinogens, drugs and pesticides.

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Section: Effect O F ' M a H On Ahh Activity In Microsomes Ofrut Liversupporting

confidence: 88%

Section: Efj'2ct Qf'mab-pb and Mah-mc On A H H Ecde And A E In Se mentioning

confidence: 65%

Section: Cj! T O Chuomes ' P-450'mentioning

confidence: 82%

Section: Prcjparution Of M a Bmentioning

confidence: 99%

Section: Prcjparution Of M a Bmentioning

confidence: 99%

See 3 more Smart Citations

Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital

Wiebel¹,

Park

Kiefer³

et al. 1984

Eur J Biochem

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In vitro Methoden zur Untersuchung des Phase-I Metabolismus

Härtter

2000

Pharmazie in unserer Zeit

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Ethanol interferes with regeneration-associated changes in biotransforming enzymes: A potential mechanism underlying ethanol's carcinogenicity?

et al. 1991

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The effects of chronic ethanol consumption on enzyme systems involved in carcinogen activation and detoxification were studied in a rat model of liver regeneration. In control rats, steady-state messenger RNAs of cytochrome P450j decreased 12 to 24 hr after partial hepatectomy but were fully recovered by 48 to 72 hr. In contrast, messenger RNA levels of cytochrome P450b and P450d did not vary significantly during that period. Steady-state messenger RNA levels for the placental form of glutathione S-transferase decreased within 30 min after partial hepatectomy but fluctuated until levels returned to normal by 48 hr. Preliminary nuclear run-on analyses suggest that the regulation of cytochrome P450j and the placental form of glutathione S-transferase messenger RNA levels involves posttranscriptional control in these animals. In ethanol-fed rats, as in controls, expression of cytochrome P450j and the placental form of glutathione S-transferase decreased transiently after partial hepatectomy. However, compared with control values, messenger RNA levels for cytochrome P450j were greater in ethanol-fed rats at each time point. Similar results were noted for placental glutathione S-transferase levels from 12 to 48 hr after partial hepatectomy. Ethanol feeding had no apparent effect on steady-state messenger RNA levels of cytochrome P450d, P450b or the multidrug-resistant gene. In both ethanol and control rats, only prehepatectomy levels of cytochrome P450 transcripts correlated with levels of the respective P450 isoenzymes. These data indicate that liver regeneration selectively decreases the steady-state messenger RNA expression of certain isoenzymes of cytochrome P450 and glutathione S-transferase.(ABSTRACT TRUNCATED AT 250 WORDS)

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Monoclonal antibodies to phenobarbital-induced rat liver cytochrome P-450

Cited by 107 publications

References 36 publications

Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital

Expression of cytochromes P-450 in rat hepatoma cells. Analysis by monoclonal antibodies specific for cytochromes P-450 from rat liver induced by 3-methylcholanthrene or phenobarbital

In vitro Methoden zur Untersuchung des Phase-I Metabolismus

Ethanol interferes with regeneration-associated changes in biotransforming enzymes: A potential mechanism underlying ethanol's carcinogenicity?

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