Monoclonal antibodies to human cartilage oligomeric matrix protein: epitope mapping and characterization of sandwich ELISA

Vilı́m, Vladimı́r; Vobůrka, Zdeněk; Vytášek, Richard; Šenolt, Ladislav; Tchetverikov, I.; Kraus, Virginia B.; Pavelka, Karel

doi:10.1016/s0009-8981(02)00375-3

Cited by 39 publications

(37 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…28 We now show that the serum concentration of COMP is stable during daytime-that is, when serum samples are normally obtained. Our study corroborates and extends a study by Vilim et al, 29 who studied COMP levels in patients with rheumatoid arthritis and osteoarthritis, in serum samples obtained at two time points, in the morning before breakfast and 2 h after lunch. These authors found that the serum levels of COMP did not change considerably between the two sample occasions in either group.…”

Section: Discussionsupporting

confidence: 92%

Diurnal variation in serum levels of cartilage oligomeric matrix protein in patients with knee osteoarthritis or rheumatoid arthritis

Andersson

Petersson

Karlsson

et al. 2006

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

Objective: To monitor changes in serum concentrations of cartilage oligomeric matrix protein (COMP) during a 24-h period to determine any diurnal variation, and to estimate the half life of COMP in the circulation in patients with symptomatic knee osteoarthritis and in those with rheumatoid arthritis. Methods: Serum samples were drawn every 4 h (7 samples/patient over 24 h) in 10 patients with knee osteoarthritis and 14 patients with rheumatoid arthritis. Osteoarthritis was defined radiographically and clinically (American College of Rheumatology (ACR) criteria) and rheumatoid arthritis according to the 1987 ACR criteria. Serum COMP was measured by sandwich ELISA. A statistical model for the diurnal variation in the COMP levels was developed using the computer program NONMEM. Results: No considerable changes in COMP levels were observed during the day between 08:00 and 21:00 in either group. A significant decrease in serum COMP was apparent during bed rest at night, reaching the lowest levels between 04:00 and 05:00 (p,0.03 or better v all other time points) in patients with osteoarthritis and in those with rheumatoid arthritis. From the rate of decreasing serum COMP levels, a putative half life of COMP in the circulation was estimated to be 7.4 h. Conclusion: During normal daytime activities, serum COMP levels are constant. The decrease during the night indicates a rapid elimination of COMP once it has reached the circulation. The stable COMP levels during the day suggest that it is not necessary to further standardise the time of serum sampling in clinical practice.

show abstract

Section: Discussionsupporting

confidence: 92%

Diurnal variation in serum levels of cartilage oligomeric matrix protein in patients with knee osteoarthritis or rheumatoid arthritis

Andersson

Petersson

Karlsson

et al. 2006

Annals of the Rheumatic Diseases

View full text Add to dashboard Cite

show abstract

“…Characteristics of these COMP fragments have previously been described using mouse monoclonal epitope mapping (32,33). However, the present report for the first time defines ending amino acids, neoepitopes, within these COMP fragments.…”

Section: Discussionmentioning

confidence: 73%

Novel Cartilage Oligomeric Matrix Protein (COMP) Neoepitopes Identified in Synovial Fluids from Patients with Joint Diseases Using Affinity Chromatography and Mass Spectrometry

Åhrman

Lorenzo

Holmgren

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: To understand molecular processes underlying cartilage destruction, we analyzed COMP fragments released into synovial fluid in joint diseases. Results: Twelve novel COMP neoepitopes have been identified. Conclusion:The release of COMP neoepitopes provides means for monitoring disease progression. Significance: Based on the specificity, selectivity, and sensitivity of each neoepitope, a new generation of biomarkers for cartilage destruction can be developed.

show abstract

“…COMP was quantified in guinea pig synovial fluid by competitive enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody 12C4. This antibody recognizes an epitope at the beginning of the COOH-terminal globular domain of human COMP (22) and cross-reacts with guinea pig COMP. The ELISA was performed as previously described (23) with slight modifications, including the use of synovial fluid (1:40 to 1:320 dilution) or standard (purified human COMP at 0.0035-0.5 g/ml) in Tween 20-phosphate buffered saline (PBS) and the 12C4 monoclonal antibody (1:500,000 dilution).…”

Section: Study Design the Institutional Animal Care And Usementioning

confidence: 99%

Ascorbic acid increases the severity of spontaneous knee osteoarthritis in a guinea pig model

Kraus

Huebner

Stäbler

et al. 2004

Arthritis & Rheumatism

View full text Add to dashboard Cite

Objective. To determine whether ascorbic acid might be of benefit for the treatment of spontaneous osteoarthritis (OA) when administered over a long period of time.Methods. We investigated the effects of 8 months' exposure to low, medium, and high doses of ascorbic acid on the in vivo development of histologic knee OA in the male Hartley guinea pig. The low dose represented the minimum amount needed to prevent scurvy. The medium dose was the amount present in standard laboratory guinea pig chow and resulted in plasma levels comparable with those achieved in a person consuming 200 mg/day (5 fruits and vegetables daily). The high dose was the amount shown in a previous study of the guinea pig to slow the progression of surgically induced OA.Results. We found an association between ascorbic acid supplementation and increased cartilage collagen content but, in contrast to findings in a previous study of surgically induced OA in the guinea pig, ascorbic acid worsened the severity of spontaneous OA. Active transforming growth factor ␤ (TGF␤) was expressed in marginal osteophytes, whose size and number were significantly increased with increasing intake of ascorbic acid. Synovial fluid levels of cartilage oligomeric matrix protein, a biomarker of cartilage turnover, corroborated the histologic findings.Conclusion. Ascorbic acid has been shown to activate latent TGF␤. Prolonged intraarticular exposure to TGF␤ has been shown to cause OA-like changes. We found expression of active TGF␤ in osteophytes, a prominent feature of the joint histology seen in association with ascorbic acid treatment. Thus, the deleterious effects of prolonged ascorbic acid exposure may be mediated in part by TGF␤. This worsening of OA with ascorbic acid supplementation suggests that ascorbic acid intake should not be supplemented above the currently recommended dietary allowance (90 mg/day for men and 75 mg/day for women).

show abstract

Monoclonal antibodies to human cartilage oligomeric matrix protein: epitope mapping and characterization of sandwich ELISA

Cited by 39 publications

References 23 publications

Diurnal variation in serum levels of cartilage oligomeric matrix protein in patients with knee osteoarthritis or rheumatoid arthritis

Diurnal variation in serum levels of cartilage oligomeric matrix protein in patients with knee osteoarthritis or rheumatoid arthritis

Novel Cartilage Oligomeric Matrix Protein (COMP) Neoepitopes Identified in Synovial Fluids from Patients with Joint Diseases Using Affinity Chromatography and Mass Spectrometry

Ascorbic acid increases the severity of spontaneous knee osteoarthritis in a guinea pig model

Contact Info

Product

Resources

About