Monoclonal antibodies to bovine platelet factor 4: Species interactions to platelets and megakaryocytes using indirect immunocytofluorescence

Ciaglowski, Raymond E.; Martinez, Joanne; Ryan, Thomas J.; Lewandowski, Leon J.; Barnhart, Marion I.; Weller, Mary A.; Chen, Shan te; Walz, Daniel A.

doi:10.1016/0049-3848(86)90384-1

Cited by 9 publications

(2 citation statements)

References 23 publications

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“…It should be noted that despite some similarities in terms of platelet surface antigens between men and rodents, it is crucial to use different antibodies for labeling human, murine, and rat blood platelets. Application of the same antibodies for these species is not indicated as any putative cross-reactivity of antibodies against human, murine, and rat platelet surface antigens is minimal or none [4]. The selection of proper antibodies can be a particular problem for the researcher, as the range of commercially available monoclonal antibodies against the rodent platelet antigens is quite limited, especially in the case of rat platelets.…”

Section: From the Beginning To The End-search For An Optimal Discrimimentioning

confidence: 99%

Only the Truth Would Enlighten Us — The Advantages and Disadvantages of Flow Cytometry as a Method of Choice in the Study of Mouse and Rat Platelets

Kassassir¹,

Siewiera²,

Przygodzki³

et al. 2016

Flow Cytometry - Select Topics

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Increasing number of transgenic and knockout strains of laboratory rodents has been developed to provide reliable models of human cardiovascular diseases. Due to apparent differences in platelet physiology, morphology, biochemistry, etc. between rodents and men, methods employed to study blood platelets in rodents should always consider these differences in a reasonably critical way. Flow cytometry is a convenient tool that enables to easily cope with the minute amounts of the available biological material and providing an extremely versatile information. This review focuses on the practical and methodological aspects of flow cytometry, pointing to the key elements of the commonly used protocols for determining of multiple parameters of blood platelet (patho)physiology in mice and rats. We summarized and critically reviewed the available procedures, as well as figured out how to overcome possible obstacles, shortcomings, drawbacks or artefacts that a researcher may encounter when monitoring various phenomena intimately associated with blood platelet biology. Flow cytometry assays have been also collated with some alternative techniques (intravital fluorescence microscopy, in vitro platelet adhesion under flow conditions). We hope that our paper may further facilitate other researchers to study mouse and rat platelets with the use of the most optimal and the least artefact-prone procedures.

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Section: From the Beginning To The End-search For An Optimal Discrimimentioning

confidence: 99%

Only the Truth Would Enlighten Us — The Advantages and Disadvantages of Flow Cytometry as a Method of Choice in the Study of Mouse and Rat Platelets

Kassassir¹,

Siewiera²,

Przygodzki³

et al. 2016

Flow Cytometry - Select Topics

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“…Previously, only Ciaglowski et al (41) presented data concerning the production of mAbs directed against PF4. They obtained a panel of mAbs specific for bovine molecule, some of them being cross-reactive with the human protein.…”

Section: The Control Represented By Fc Fragments (A-a)mentioning

confidence: 99%

Neutralization of the Antiheparin Activity of Platelet Factor 4 by a Monoclonal Antibody

et al. 1992

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SummaryWe have produced a panel of monoclonal antibodies (mAbs) against rabbit platelet factor 4 (PF4). Two of these mAbs have been characterized in this study. In particular the antibody called 10B2, which also recognizes the human molecule, is able to block PF4’s ability to neutralize heparin in a modified Heparin-Factor Xa chromogenic assay. The inhibition appears to be more than 95% at 1:1 mAb/PF4 molar ratio both for purified rabbit and human PF4. Similar results were obtained using supernatants from stimulated human platelets (90% of inhibition at 1:1 mAb/ PF4 molar ratio) or using Fab fragments from 10B2. Studies to determine the antigenic determinant against which 10B2 is directed, show that this is an assembled epitope which involves disulfide bonds of the PF4.

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Monoclonal Antibodies in Blood Coagulation

Prowse¹

1988

Biotechnology in Blood Transfusion

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Monoclonal antibodies to bovine platelet factor 4: Species interactions to platelets and megakaryocytes using indirect immunocytofluorescence

Cited by 9 publications

References 23 publications

Only the Truth Would Enlighten Us — The Advantages and Disadvantages of Flow Cytometry as a Method of Choice in the Study of Mouse and Rat Platelets

Only the Truth Would Enlighten Us — The Advantages and Disadvantages of Flow Cytometry as a Method of Choice in the Study of Mouse and Rat Platelets

Neutralization of the Antiheparin Activity of Platelet Factor 4 by a Monoclonal Antibody

Monoclonal Antibodies in Blood Coagulation

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