Monoclonal antibodies that distinguish between New World species of Leishmania

Pratt, Diane McMahon; David, John R.

doi:10.1038/291581a0

Cited by 179 publications

(26 citation statements)

References 11 publications

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“…This result supports the finding that L. mexicana and L. braziliensis can be differentiated by using speciesspecific monoclonal antibodies (29,30 …”

Section: Resultssupporting

confidence: 81%

Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies.

Fong¹,

Chang²

1982

Proc. Natl. Acad. Sci. U.S.A.

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The fusion of SP2/O myeloma cells with spleen cells from mice immunized with Leishmania msexcana amazonensw promastigotes produced hybridoma clones. Indirect immunofluorescent antibody assay with live leishmanias showed that the monoclonal antibody 6H12 recognized only the antigens bound to the surface of L. mexicana amazonensus promastigotes. It also showed that the antibody bound to neither amastigotes ofthis species nor to other Leishmania species-i.e., L. braziliensis braziliensis, L. tropica, and L. donovani. Monoclonal antibodies from three other clones (4D11, 4H9, and 6A11) were found to compete with 6H12 for binding to L. mexicana promastigotes. With lysates of [asS]methionine-labeled promastigotes, all four monoclonal antibodies precipitated the same triplet set of protein bands at the -68,000-dalton region, whereas another monoclonal antibody (6G5) precipitated a different band at -90,000 daltons. During differentiation of L. mexicana amazonensis from amastigotes to promastigotes, there was a 4-to 8-fold increase above the initial level in the binding of 6H12 monoclonal antibody to leishmanias, as detected by enzyme-linked immunosorbent assay and quantitative fluorometric assay, respectively. Thus, we have demonstrated the use ofmonoclonal antibodies as probes for antigens that change during leishmanial differentiation.

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“…This result supports the finding that L. mexicana and L. braziliensis can be differentiated by using speciesspecific monoclonal antibodies (29,30 …”

Section: Resultssupporting

confidence: 81%

Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies.

Fong¹,

Chang²

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…Isoenzyme electrophoresis was performed to identify the species for the strains not accurately typed by reactivity to monoclonal antibodies (Pratt and David, 1981; McMahon-Pratt et al 1982; Saravia et al 1998). …”

Section: Methodsmentioning

confidence: 99%

Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR

et al. 2014

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SUMMARY Variation in clinical accuracy of molecular diagnostic methods for cutaneous leishmaniasis (CL) is commonly observed depending on the sample source, the method of DNA recovery and the molecular test. Few attempts have been made to compare these variables. Two swab and aspirate samples from lesions of patients with suspected CL (n = 105) were evaluated alongside standard diagnosis by microscopic detection of amastigotes or culture of parasites from lesion material. Three DNA extraction methods were compared: Qiagen on swab and aspirate specimens, Isohelix on swabs and Boil/Spin of lesion aspirates. Recovery of Leishmania DNA was evaluated for each sample type by real-time polymerase chain reaction detection of parasitic 18S rDNA, and the diagnostic accuracy of the molecular method determined. Swab sampling combined with Qiagen DNA extraction was the most efficient recovery method for Leishmania DNA, and was the most sensitive (98%; 95% CI: 91–100%) and specific (84%; 95% CI: 64–95%) approach. Aspirated material was less sensitive at 80% (95% CI: 70–88%) and 61% (95% CI: 50–72%) when coupled to Qiagen or Boil-Spin DNA extraction, respectively. Swab sampling of lesions was painless, simple to perform and coupled with standardized DNA extraction enhances the feasibility of molecular diagnosis of CL.

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“…Isolates were tested with a modified indirect immunofluorescence technique using a fluorochrome conjugated with avidin/biotin accordingly as previously described 31 with 23 leishmanial-specific monoclonal antibodies: B2, B5, B12, B11, B13, B18, B19, CO1, CO2, CO3, D13, L1, LA2, M2, N2, N3, V1, WA2, W1, W2, WH1, WIC, and T3. 32,33 Promastigotes of L. infantum isolated from a patient with kala-azar from Brazil were used to construct the standard curve. Initially, the DNA was extracted from 10 6 parasites and the DNA concentration and purity were measured in a NanoDrop 2000 Spectrophotometer (Wilmington, DE).…”

Section: Patientsmentioning

confidence: 99%

Bone Marrow Parasite Burden among Patients with New World Kala-Azar is Associated with Disease Severity

Silva¹,

Zacarias

Figueirêdo

et al. 2014

The American Society of Tropical Medicine and Hygiene

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Abstract. Kala-azar or visceral leishmaniasis, found mostly throughout the Indian Subcontinent, East Africa, and Brazil, kills 20,000-40,000 persons annually. The agents, Leishmania donovani and Leishmania infantum, are obligatory intracellular protozoa of mononuclear phagocytes found principally in the spleen and bone marrow. Protracted fever, anemia, wasting, hepatosplenomegaly, hemorrhages, and bacterial co-infections are typical features. One hundred and twenty-two (122) in-hospital patients were studied to verify if higher bone marrow parasite load estimated by quantitative polymerase chain reaction is associated with severe disease. The estimated median parasite load was 5.0 parasites/ 10 6 human nucleated cells. It is much higher in deceased than among survivors (median 75.0 versus 4.2). Patients who lost more weight had a higher parasite burden, as well as patients with epistaxis, abdominal pain, edema, and jaundice. This study suggests that higher parasite load is influenced by wasting, which may lead to more severe disease.

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Monoclonal antibodies that distinguish between New World species of Leishmania

Cited by 179 publications

References 11 publications

Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies.

Surface antigenic change during differentiation of a parasitic protozoan, Leishmania mexicana: Identification by monoclonal antibodies.

Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR

Bone Marrow Parasite Burden among Patients with New World Kala-Azar is Associated with Disease Severity

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