Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants

Anderton, BH; Breinburg, D; Downes, MJ; Green, PJ; Be, Tomlinson; Ulrich, J.; Wood, JN; Kahn, J.

doi:10.1097/00002093-198701040-00019

Cited by 56 publications

(70 citation statements)

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“…Solvents were distilled before use. (Anderton et al, 1982) and mouse glial fibrillary acidic protein (GFAP) antiserum were a gift of Dr. F. S. Walsh (Institute of Neurology, The National Hospital, London, UK).…”

Section: Methodsmentioning

confidence: 99%

Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures: II. Modulatory effects of gangliosides

et al. 1988

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This paper analyzes the effects of exogenously supplied GM1 on the development, i.e., specific neurotransmitter uptake capability and survival, of the dopaminergic neurons present in fetal mouse- dissociated mesencephalic cells. Exogenous GM1, but not asialo-GM1, sialic acid, or the oligosaccharide chain of GM1, enhances in a time- and concentration-dependent manner the specific 3H-dopamine uptake (increase of the apparent Vmax and decrease of the apparent Km value) and the long-term survival of the dopaminergic neurons. The GM1 effects on the behavior of the dopaminergic neurons require the presence of cell-derived neuronotrophic influences present within the culture system and are associated with an increase in the response of the cells to the trophic influences. GM1 effects are not limited to dopaminergic neurons, and depend on the stable association of the ganglioside molecule with the cells. It is suggested that GM1 is not a trophic agent per se, but rather potentiates neuronotrophic activities and/or exerts independent influences to which neurons respond only if appropriately supported.

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Section: Methodsmentioning

confidence: 99%

Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures: II. Modulatory effects of gangliosides

et al. 1988

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“…Monoclonal antibody recognizes polyphosphorylated epitopes on neurofilaments and other molecules such as rhodopsin (Anderton et al, 1982). In mouse retina, this antibody mainly labeled processes of horizontal cells and axons of ganglion cells (Dräger et al, 1984;Inoue et al, 2002).…”

Section: Anti-sv2mentioning

confidence: 99%

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Ricatti

Alfie

Lavoie

et al. 2008

Synapse

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Here we show a detailed immunocytochemical localization of two enzymes of the ENTPDase family in the retinal layers of two vertebrate species, namely, the mouse and the zebrafish. In the mouse retina, NTPDase2 was chiefly localized in Mü ller glia and ganglion cell processes. NTPDase1 was located on neurons as well, since it was expressed by horizontal and ganglion cell processes, suggesting that nucleotides such as ATP and ADP can be hydrolyzed at the surface of these cells. NTPDase1 was also detected in intraretinal blood vessels of the mouse. Regarding zebrafish, NTPDases1 and 2 seem to be differentially localized in horizontal cell processes, photoreceptor segments, and ganglion cell dendrites and axons, but absent from Mü ller glia. Moreover, NTPDases1 and 2 appear to be expressed within the germinal margin of the zebrafish retina that contains proliferative and differentiating cells. Retinal homogenates from both species exhibited ecto-ATPase activity which might be attributed at least to NTPDases1 and 2, whose expression is described in this report. Our results suggest a compartmentalized regulation of extracellular nucleotide/nucleoside concentration in the retinal layers, supporting a relevant role for extracellular nucleotide mediated-signaling in vertebrate retinas. Synapse 63:291-307,

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“…Immunofluorescence and immuno-EM Neurons were fixed and processed for biotin immunoreactivity with a rabbit polyclonal anti-biotin antibody (1:100 dilution in Tris-buffered saline), monoclonal antibodies (Sternberger Monoclonals, Bethesda, MD) directed against phosphorylated epitopes (SMI-31) and nonphospho-epitopes (SMI-32) shared by NF-H and NF-M, a monoclonal antibody (RT97) directed against developmentally delayed phosphorylated epitopes of NF-H (Anderton et al, 1982) or a monoclonal anti-NF-H antibody (generous gift of Virginia Lee University of Pennsylvania, PA, USA), followed by either goat antirabbit or goat anti-mouse secondary antibody conjugated to Texas Red at a 1:150 dilution (Sigma). Microinjected neurons were fixed 2 hours after injection.…”

Section: Microinjection and Transfectionmentioning

confidence: 99%

Cdk5 regulates axonal transport and phosphorylation of neurofilaments in cultured neurons

Shea

Yabe

Ortiz

et al. 2004

Journal of Cell Science

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Phosphorylation has long been considered to regulate neurofilament (NF) interaction and axonal transport, and, in turn, to influence axonal stability and their maturation to large-caliber axons. Cdk5, a serine/threonine kinase homologous to the mitotic cyclin-dependent kinases, phosphorylates NF subunits in intact cells. In this study, we used two different haptenized NF subunits and manipulated cdk5 activity by microinjection, transfection and pharmacological inhibition to monitor the effect of Cdk5-p35 on NF dynamics and transport. We demonstrate that overexpression of cdk5 increases NF phosphorylation and inhibits NF axonal transport, whereas inhibition both reduces NF phosphorylation and enhances NF axonal transport in cultured chicken dorsal-root-ganglion neurons. Large phosphorylated-NF `bundles' were prominent in perikarya following cdk5 overexpression. These findings suggest that Cdk5-p35 activity regulates normal NF distribution and that overexpression of Cdk5-p35 induces perikaryal accumulation of phosphorylated-NFs similar to those observed under pathological conditions.

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Monoclonal antibodies show that neurofibrillary tangles and neurofilaments share antigenic determinants

Cited by 56 publications

References 0 publications

Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures: II. Modulatory effects of gangliosides

Development and survival of neurons in dissociated fetal mesencephalic serum-free cell cultures: II. Modulatory effects of gangliosides

Immunocytochemical localization of NTPDases1 and 2 in the neural retina of mouse and zebrafish

Cdk5 regulates axonal transport and phosphorylation of neurofilaments in cultured neurons

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