Monoclonal antibodies recognize localized antigens in the eye and central nervous system of the marine snail Bulla gouldiana.

Bedian, Vahe; Chen, Yuli; Roberts, Michael H.

doi:10.1177/39.3.1993829

Cited by 3 publications

(4 citation statements)

References 11 publications

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“…In the mollusk Bullu, a screen for antibodies to the eye, which is believed to contain this animal's circadian pacemaker (Jacklet, 1985) produced a monoclonal antibody that recognized an antigen present in the putative pacemaker cells; this antigen exhibited a circadian fluctuation in abundance. In addition to these cells, the antibody also labeled what are believed to be glial processes in the CNS and the eyes (Roberts et al, 1989;Bedian et al, 1991). Our results suggest that glial cells may likewise play a role in pacemaker function in Drosophila. The everincreasing number of tools (e.g., Bier et al, 1989) and markers (e.g., Buchner et al, 1988;Buchner, 1991) that are becoming available in Drosophila for the study of CNS development and structure should allow the nature of per-expressing cells to be further investigated.…”

Section: Period (Hrs)mentioning

confidence: 56%

Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms

et al. 1992

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The product of the period (per) gene of Drosophila melanogaster is continuously required for the functioning of the circadian pacemaker of locomotor activity. We have used internally marked mosaics to determine the anatomical locations at which per expression is required for adult rhythmicity, and thus where the fly's circadian pacemaker is likely located in this holometabolous insect. We first provide a detailed description of the distribution and nature of per-expressing cells in the fly's CNS. Using an antibody to the per gene product, or to that of a reporter of per expression, in conjunction with an antibody to the embryonic lethal-abnormal visual system (elav) gene product--which is used as a marker of neuronal identity--we have experimentally confirmed previously proposed assignments of per-expressing cells to the neuronal and glial classes. Thus, we found that per expression and elav immunoreactivity colocalized in large cells located in the lateral cortex of the central brain, as well as in more dorsally located cells in the posterior central brain. In contrast, we found that cells located at the margins of the cortex and the neuropil, and within the neuropil, as well as smaller cortical cells found throughout the brain's cortex, were elav negative, supporting the notion that they are glial in nature. Using internally marked mosaics, we find that the pacemaker is located in brain but is not exclusive to the eyes, the ocelli, or the optic lobes, which is consistent with previous reports obtained in this and other insects of this class. Although the pacemaker may be a paired structure, we show that the functioning of one of them is sufficient for rhythmicity. Finally, we report that glial expression is sufficient for some behavioral rhythmicity to be manifest. However, the rhythmicities of animals for which per expression was confined to glia were weak, suggesting that neuronal per expression as well may be required for normal pacemaker function.

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Section: Period (Hrs)mentioning

confidence: 56%

Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms

et al. 1992

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“…There are indications that glial cells may play a role in the pacemaker function of the mammalian suprachiasmatic nuclei (SCN) (35)(36)(37), of the eye of Bulla (38,39), and also in Drosophila (11). In the mammalian SCN, glial cells show ultradian oscillations of calcium concentration (as observed in the SCN neurons), which can be influenced by neurotransmitters from the SCN neurons (36).…”

Section: Discussionmentioning

confidence: 99%

“…If glial activity is disrupted by different agents, the in vitro circadian rhythm of neural activity and the in vivo circadian activity rhythm are also disturbed (37). In the mollusk Bulla, antigens exhibiting circadian fluctuations in their abundance were present in pacemaker and in glial cells (38,39). In Drosophila, PER-containing glial cells exhibit circadian fluctuations in the abundance of PER as do the PER-containing neurons (9,10).…”

Section: Discussionmentioning

confidence: 99%

The period clock gene is expressed in central nervous system neurons which also produce a neuropeptide that reveals the projections of circadian pacemaker cells within the brain of Drosophila melanogaster.

Helfrich‐Förster

1995

Proc. Natl. Acad. Sci. U.S.A.

377

307

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The period clock gene is expressed in central nervous system neurons which also produce a neuropeptide that reveals the projections of circadian pacemaker cells within ABSTRACTThe period protein (PER) is an essential component of the circadian clock in Drosophila melanogaster. Although PER-containing pacemaker cells have been previously identified in the brain, the neuronal network that comprises the circadian clock remained unknown. Here it is shown that some PER neurons are also immunostained with an antiserum against the crustacean pigment-dispersing hormone (PDH). This antiserum reveals the entire arborization pattern of these pacemaker cells. The arborizations of these neurons are appropriate for modulation of the activity of many neurons and they might interact with PER-containing glial cells. A putative physiological role of PDH in the circadian system is discussed.

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“…, 1973Nagle et al. 1989a, b;Baux et al 1990;Bedian et al. 1991;Hickmott and Carew, 1991;Ziv et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Changes Occur in the Central Nervous System of the Nudibranch Berghia verrucicornis (Mollusca, Opisthobranchia) During Metamorphosis

Carroll

Kempf

1994

The Biological Bulletin

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The structure of the larval and juvenile central nervous system (CNS) in Berghia verrucicornis, an aeolid nudibranch, was examined using 1-{mu}m serial sections. The CNS consists of paired optic, cerebral, pleural (also known as sub- and supra-intestinal ganglia), pedal, and buccal ganglia, and a single visceral ganglion. A pleurovisceral loop is present. The organization of the CNS changes as the nudibranch undergoes metamorphosis. In general, there is a condensation of the CNS. The cerebral and pleural ganglia fuse to form the prominent cerebropleural ganglia. The single visceral ganglion fuses with the pleural portion of the left cerebropleural ganglion. The buccal ganglia enlarge and fully organize into a cortex of nerve cell bodies and medulla of nerve fibers. Rhinophoral ganglia develop anterior to each cerebropleural ganglion and a pair of nervous processes extend from each: one to the developing rhinophore and the other anteroventral toward the mouth and associated structures. These metamorphic changes are similar to those seen in other commonly studied opisthobranch species, suggesting that Berghia verrucicornis is an appropriate model for the developmental examination of structure and function in molluscan nervous systems.

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Monoclonal antibodies recognize localized antigens in the eye and central nervous system of the marine snail Bulla gouldiana.

Cited by 3 publications

References 11 publications

Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms

Expression of the period clock gene within different cell types in the brain of Drosophila adults and mosaic analysis of these cells' influence on circadian behavioral rhythms

The period clock gene is expressed in central nervous system neurons which also produce a neuropeptide that reveals the projections of circadian pacemaker cells within the brain of Drosophila melanogaster.

Changes Occur in the Central Nervous System of the Nudibranch Berghia verrucicornis (Mollusca, Opisthobranchia) During Metamorphosis

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