Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968–1973, 1977–1993 and 1997–2003

Okada, Jun; Ohshima, Nobuharu; Kubota‐Koketsu, Ritsuko; Ota, Sayuri; Takase, Wakana; Azuma, Masachika; Iba, Yoshitaka; Nakagawa, Naoko; Yoshikawa, Tetsushi; Nakajima, Youichi; Ishikawa, Takahiro; Asano, Yoshizo; Okuno, Yoshinobu; Kurosawa, Yoshikazu

doi:10.1016/j.virol.2009.11.025

Cited by 23 publications

(38 citation statements)

References 20 publications

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“…The limited V H gene usage we have found in donor H is similar in extent to the response detected to vaccination by Moody et al (41), with up to 12 V H -related sets in any one individual; however, our data contrast with data from Okada et al (42,43), who isolated 221 unique V H sequences that reacted with H3 HA by phage display from a single individual, who, unlike our donor, Reactivity with A/USSR/90/1977. The high frequency of somatic mutations in the 4 sets of monoclonal antibodies from donor H that recognize K163 suggested that they may have been selected from the memory B cell pool (33).…”

Section: Discussionsupporting

confidence: 63%

Focused antibody response to influenza linked to antigenic drift

Huang

Rijal

Schimanski

et al. 2015

Journal of Clinical Investigation

123

129

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Section: Discussionsupporting

confidence: 63%

Focused antibody response to influenza linked to antigenic drift

Huang

Rijal

Schimanski

et al. 2015

Journal of Clinical Investigation

123

129

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“…Clones that bound to virus particles were isolated, and their binding and neutralizing activities were examined. We previously reported the results obtained from the donor born in 1960 (16,17). In the present report, we focus on clones with extremely broad strain specificity that were isolated from the donor born in 1974.…”

mentioning

confidence: 99%

“…Screening of Ab library. Phage bound to virus particles was selected by a panning method as described previously (16). In brief, formalin-treated virus particles were used as antigens (Ags) in the screenings.…”

mentioning

confidence: 99%

Naturally Occurring Antibodies in Humans Can Neutralize a Variety of Influenza Virus Strains, Including H3, H1, H2, and H5

Ohshima

Iba

Kubota‐Koketsu

et al. 2011

J Virol

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100

114

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Influenza A viruses are classified into 16 subtypes according to the serotypes of hemagglutinin (HA). It is generally thought that neutralizing antibodies (Abs) are not broadly cross-reactive among HA subtypes. We examined the repertoire of neutralizing Abs against influenza viruses in humans. B lymphocytes were collected from donors by apheresis, and Ab libraries were constructed by using phage-display technology. Anti-HA clones were isolated by screening with H3N2 viruses. Their binding activity was examined, and four kinds of Abs showing broad strain specificity were identified from one donor. Two of the Abs, F045-092 and F026-427, were extensively analyzed. They neutralized not only H3N2 but also H1N1, H2N2, and H5N1 viruses, although the activities were largely varied. Flow cytometry suggested that they have the ability to bind to HA and HA1 artificially expressed on the cell surface. They show hemagglutination inhibition activity and do not compete with C179, an Ab thought to bind to the stalk region. F045-092 competes with Abs that recognize sites A and B for binding to HA. Furthermore, the serine at residue 136 in site A could be a part of the epitope. Thus, it is likely that F045-092 and F026-427 bind to a conserved epitope in the head region formed by HA1. Interestingly, while the V H 1-69 gene can encode MAbs against the HA stem that are group 1 specific, F045-092 and its relatives that recognize the head region also use V H 1-69. The possible epitope recognized by these clones is discussed.

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“…One MAb, designated S139/1, was reported to neutralize not only H3 viruses but also H1, H2, and H13 viruses through binding of antigenic site B (52). Human H3 MAbs have been created using phage display (23,24,29), plasmablast single cell cloning (46), or hybridoma technology (50,53). Murine MAbs against 1957 H2 also have been made (21,35,39) and used for antigenic mapping (28,35,49), including an antibody directed to an HA stem region epitope that is preserved across influenza virus phylogenetic group 1 HAs (25).…”

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confidence: 99%

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

Krause

Tsibane

Tumpey

et al. 2012

J Virol

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Investigation of the human antibody response to the 1957 pandemic H2N2 influenza A virus has been largely limited to serologic studies. We generated five influenza virus hemagglutinin (HA)-reactive human monoclonal antibodies (MAbs) by hybridoma technology from the peripheral blood of healthy donors who were born between 1950 and 1968. Two MAbs reacted with the pandemic H2N2 virus, two recognized the pandemic H3N2 virus, and remarkably, one reacted with both the pandemic H2N2 and H3N2 viruses. Each of these five naturally occurring MAbs displayed hemagglutination inhibition activity, suggesting specificity for the globular head domain of influenza virus HA. When incubated with virus, MAbs 8F8, 8M2, and 2G1 each elicited H2N2 escape mutations immediately adjacent to the receptor-binding domain on the HA globular head in embryonated chicken eggs. All H2N2-specific MAbs were able to inhibit a 2006 swine H2N3 influenza virus. MAbs 8M2 and 2G1 shared the V H 1-69 germ line gene, but these antibodies were otherwise not genetically related. Each antibody was able to protect mice in a lethal H2N2 virus challenge. Thus, even 43 years after circulation of H2N2 viruses, these subjects possessed peripheral blood B cells encoding potent inhibiting antibodies specific for a conserved region on the globular head of the pandemic H2 HA.

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Monoclonal antibodies in man that neutralized H3N2 influenza viruses were classified into three groups with distinct strain specificity: 1968–1973, 1977–1993 and 1997–2003

Cited by 23 publications

References 20 publications

Focused antibody response to influenza linked to antigenic drift

Focused antibody response to influenza linked to antigenic drift

Naturally Occurring Antibodies in Humans Can Neutralize a Variety of Influenza Virus Strains, Including H3, H1, H2, and H5

Human Monoclonal Antibodies to Pandemic 1957 H2N2 and Pandemic 1968 H3N2 Influenza Viruses

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