Monoclonal antibodies for detection of Histoplasma capsulatum variety capsulatum antigen in patients with histoplasmosis

Fojtasek, M F; Kamel, S; Karimi, S T; Connolly‐Stringfield, Patricia; Wheat, L J

doi:10.1016/0888-0786(93)90038-2

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Currently, the RIA uses rabbit polyclonal antisera, although monoclonal antibodies have been used in an attempt to replace the polyclonal sera. However, the results arising from this modification were not reported as being satisfactory (5).…”

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confidence: 89%

Development of a novel antigen detection test for histoplasmosis

et al. 1997

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Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals living or travelling in areas of endemicity, who, without antifungal therapy, may develop a progressive disseminated fatal infection. For such patients, the detection of antibody responses by immunodiffusion or complement fixation test is of limited use. In contrast, the detection of Histoplasma capsulatum circulating antigens may provide a more practical approach to the rapid diagnosis of the disease. Accordingly, an inhibition enzyme-linked immunosorbent assay (ELISA) for the detection of a 69-to 70-kDa H. capsulatumspecific determinant and incorporating a species-specific murine monoclonal antibody was developed. With sera from patients with different forms of the disease (n ‫؍‬ 35), the overall sensitivity of the test was found to be 71.4%, while the specificity was found to be 98% with normal human sera from areas of endemicity (n ‫؍‬ 44) and 85.4% with sera from patients with other chronic fungal or bacterial infections (n ‫؍‬ 48). This novel, highly specific ELISA provides a significant addition to the existing diagnostic tests for the detection of histoplasmosis.

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confidence: 89%

Development of a novel antigen detection test for histoplasmosis

et al. 1997

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“…Also, those investigators used a monoclonal anti- body to an H. capsulatum protein antigen whereas in this investigation we used a polyclonal antibody to whole H. capsulatum. While we would have preferred to use a monoclonal antibody, we have been unable to prepare monoclonal antibodies capable of detecting the carbohydrate antigen found in patients (3).…”

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confidence: 99%

Comparison of an Established Antibody Sandwich Method with an Inhibition Method of Histoplasma capsulatum Antigen Detection

Garringer¹,

Wheat

Brizendine³

2000

J Clin Microbiol

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The Histoplasma antigen immunoassay utilizes an antibody sandwich method that provides a rapid and reliable means of diagnosing the more severe forms of histoplasmosis. Inhibition assays have been developed for antigen detection and offer at least one potential advantage, namely, reduced antibody requirements. We have developed an inhibition assay using the polyclonal antibody employed in our standard sandwich assay. Urine and serum specimens from patients with culture-proven histoplasmosis and controls were tested using both methods. The two methods had similar sensitivities for detection of antigen in urine (antibody sandwich = 92.5% versus inhibition = 87.5%, P = 0.500) and serum (82.5% versus 80.0%, P = 1.000). With serum, the specificities of both methods were similar (antibody sandwich assay = 95.0% versus inhibition assay = 92.5%,P = 1.000), and with urine, the specificity of the antibody sandwich method was superior (97.5% versus 80.0%,P = 0.039). While the overall reproducibility of both methods was excellent (with urine, antibody sandwich assay intraclass correlation coefficient = 0.9975 and with serum = 0.9949; correlation coefficient of the inhibition assay with urine = 0.9736 and with serum = 0.9850), that of the inhibition method was only fair to poor for the controls: urine = −0.0152, serum = 0.5595. Reproducibility was good for the controls using the sandwich method: urine = 0.7717, serum = 0.9470. Cross-reactivity was observed in specimens from patients infected with Blastomyces dermatitidis,Paracoccidioides brasiliensis, and Penicillium marneffei. In conclusion, the decreased specificity and inferior reproducibility with control specimens suggest that the inhibition assay has poorer precision toward the lower end of the detection range.

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