Monoclonal antibodies directed against the calcium binding protein Calbindin D-28k

Celio, Marco R.; Baier, Wolfgang; Schärer, Lukas; Gregersen, H.; Viragh, Pierre A. de; Norman, Anthony W.

doi:10.1016/0143-4160(90)90014-l

Cited by 275 publications

(124 citation statements)

References 12 publications

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“…Various antibodies to calbindin D-28k have been used to immunostain horizontal cells in mammalian retinae (Rö-hrenbeck et al, 1987;Lyser et al, 1994;Peichl and González-Soriano, 1994;Massey and Mills, 1996;Johnson and Vardi, 1998;Haverkamp and Wässle, 2000;Völgyi and Bloomfield, 2002;Raven and Reese, 2003;Loeliger and Rees, 2005). The labeling patterns of horizontal cells in rabbit retina produced were similar with the mouse and rabbit calbindin antibodies that were used: mouse monoclonal antibody against calbindin (1:2,500 -5,000; Sigma; C9848, clone CB-955; Celio et al, 1990) and rabbit polyclonal antibody against calbindin (1:1,000; Swant, Bellinzona, Switzerland; CB38; Garcia-Segura et al, 1984).…”

Section: Antibodiesmentioning

confidence: 89%

Cellular distribution and subcellular localization of molecular components of vesicular transmitter release in horizontal cells of rabbit retina

Hirano

Brandstätter

Brecha

2005

J of Comparative Neurology

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The mechanism underlying transmitter release from retinal horizontal cells is poorly understood. We investigated the possibility of vesicular transmitter release from mammalian horizontal cells by examining the expression of synaptic proteins that participate in vesicular transmitter release at chemical synapses. Using immunocytochemistry, we evaluated the cellular and subcellular distribution of complexin I/II, syntaxin-1, and synapsin I in rabbit retina. Strong labeling for complexin I/II, proteins that regulate a late step in vesicular transmitter release, was found in both synaptic layers of the retina, and in somata of A-and B-type horizontal cells, of ␥-aminobutyric acid (GABA)-and glycinergic amacrine cells, and of ganglion cells. Immunoelectron microscopy demonstrated the presence of complexin I/II in horizontal cell processes postsynaptic to rod and cone ribbon synapses. Syntaxin-1, a core protein of the soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) complex known to bind to complexin, and synapsin I, a synaptic vesicle-associated protein involved in the Ca 2ϩ -dependent recruitment of synaptic vesicles for transmitter release, were also present in the horizontal cells and their processes at photoreceptor synapses. Photoreceptors and bipolar cells did not express any of these proteins at their axon terminals. The presence of complexin I/II, syntaxin-1, and synapsin I in rabbit horizontal cell processes and tips suggests that a vesicular mechanism may underlie transmitter release from mammalian horizontal cells.

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Section: Antibodiesmentioning

confidence: 89%

Cellular distribution and subcellular localization of molecular components of vesicular transmitter release in horizontal cells of rabbit retina

Hirano

Brandstätter

Brecha

2005

J of Comparative Neurology

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“…Antibodies directed against the calcium-binding proteins were characterized by Celio's group. Thus, immunoblotting elucidated single protein bands of 12, 28 or 29 kDa recognized by mouse anti-PARV (Celio et al, 1988), mouse anti-CALB (Celio et al, 1990) and rabbit anti-CALR (Schwaller et al, 1993), respectively. Western blotting with rabbit anti-TH revealed a single protein band of about 60 kDa, whereas sheep anti-TrpH exclusively recognized a band of approximately 55 kDa (Chemicon/Millipore).…”

Section: Characterization Of Antibodiesmentioning

confidence: 99%

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Riedel

Westerholz

Braun

et al. 2008

Journal of Chemical Neuroanatomy

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The lateral septum (LS) plays a role in the adjustment of behavioral responses according to environmental demands. This is a complex integrative process wherein a variety of modulatory systems, i.e. cholinergic, dopaminergic and serotonergic projections forming pericellular baskets around LS neurons, are involved.Recently, vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (-ir) structures outlining unlabeled somata and their proximal dendrites were described in the LS. However, the vesicular transporters for acetylcholine and GABA were not or only rarely co-expressed with VGLUT3.In this study, the morphology and distribution of these VGLUT3-ir structures were systematically analyzed revealing that (1) they form distinct pericellular baskets (PBs) displaying variable shapes, (2) they are arranged in a layer-like pattern similar to the terminals of other modulatory systems, (3) beside a few exceptions (e.g., choline acetyltransferase), they are generally not or very sparsely colocalized with other neurochemical markers characterizing major neuron populations or afferent systems of the LS, i.e. calcium-binding proteins, tyrosine hydroxylase, tryptophan hydroxylase, vesicular glutamate transporters 1 (VGLUT1) and 2 (VGLUT2) and the vesicular GABA transporter.Thus, in the LS, a separate population of neurons is covered by VGLUT3-ir PBs. The distribution pattern and the lack of co-localization indicate that the VGLUT3-expressing cells of origin are located in the brainstem and that they could be pure glutamatergic projection neurons-different from the well-defined canonical VGLUT1-and VGLUT2-expressing neurons. Alternatively, they could

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“…300, Swant) is a mouse IgG1 (Celio et al, 1990). It recognizes a single band of 28 kDa in Western blots of different vertebrate species including mice and rats (manufacturer's data).…”

Section: Antibody Characterizationmentioning

confidence: 99%

Distribution and targets of the relaxin‐3 innervation of the septal area in the rat

Olucha-Bordonau¹,

Otero-García²,

Sánchez-Pérez³

et al. 2012

J of Comparative Neurology

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Neural tracing studies have revealed that the rat medial and lateral septum are targeted by ascending projections from the nucleus incertus, a population of tegmental GABA neurons. These neurons express the relaxin‐family peptide, relaxin‐3, and pharmacological modulation of relaxin‐3 receptors in medial septum alters hippocampal theta rhythm and spatial memory. In an effort to better understand the basis of these interactions, we have characterized the distribution of relaxin‐3 fibers/terminals in relation to different septal neuron populations identified using established protein markers. Dense relaxin‐3 fiber plexuses were observed in regions of medial septum containing hippocampal‐projecting choline acetyltransferase (ChAT)‐, neuronal nitric oxide synthase (nNOS)‐, and parvalbumin (PV)‐positive neurons. In lateral septum (LS), relaxin‐3 fibers were concentrated in the ventrolateral nucleus of rostral LS and the ventral nucleus of caudal LS, with sparse labeling in the dorsolateral and medial nuclei of rostral LS, dorsal nucleus of caudal LS, and ventral portion nuclei. Relaxin‐3 fibers were also observed in the septofimbrial and triangular septal nuclei. In the medial septum, we observed relaxin‐3‐immunoreactive contacts with ChAT‐, PV‐, and glutamate decarboxylase‐67‐positive neurons that projected to hippocampus, and contacts between relaxin‐3 terminals and calbindin‐ and calretinin‐positive neurons. Relaxin‐3 colocalized with synaptophysin in nerve terminals in all septal areas, and ultrastructural analysis revealed these terminals were symmetrical and contacted spines, somata, dendritic shafts, and occasionally other axonal terminals. These data predict that this GABA/peptidergic projection modulates septohippocampal activity and hippocampal theta rhythm related to exploratory navigation, defensive and ingestive behaviors, and responses to neurogenic stressors. J. Comp. Neurol. 520:1903–1939, 2012. © 2011 Wiley Periodicals, Inc.

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Monoclonal antibodies directed against the calcium binding protein Calbindin D-28k

Cited by 275 publications

References 12 publications

Cellular distribution and subcellular localization of molecular components of vesicular transmitter release in horizontal cells of rabbit retina

Cellular distribution and subcellular localization of molecular components of vesicular transmitter release in horizontal cells of rabbit retina

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Distribution and targets of the relaxin‐3 innervation of the septal area in the rat

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