Monoclonal antibodies directed against the calcium binding protein parvalbumin

Celio, Marco R.; Baier, Wolfgang; Schärer, Lukas; Viragh, Pierre A. de; Gerday, Ch.

doi:10.1016/0143-4160(88)90027-9

Cited by 383 publications

(176 citation statements)

References 11 publications

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“…Antibodies directed against the calcium-binding proteins were characterized by Celio's group. Thus, immunoblotting elucidated single protein bands of 12, 28 or 29 kDa recognized by mouse anti-PARV (Celio et al, 1988), mouse anti-CALB (Celio et al, 1990) and rabbit anti-CALR (Schwaller et al, 1993), respectively. Western blotting with rabbit anti-TH revealed a single protein band of about 60 kDa, whereas sheep anti-TrpH exclusively recognized a band of approximately 55 kDa (Chemicon/Millipore).…”

Section: Characterization Of Antibodiesmentioning

confidence: 99%

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Riedel

Westerholz

Braun

et al. 2008

Journal of Chemical Neuroanatomy

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The lateral septum (LS) plays a role in the adjustment of behavioral responses according to environmental demands. This is a complex integrative process wherein a variety of modulatory systems, i.e. cholinergic, dopaminergic and serotonergic projections forming pericellular baskets around LS neurons, are involved.Recently, vesicular glutamate transporter 3 (VGLUT3)-immunoreactive (-ir) structures outlining unlabeled somata and their proximal dendrites were described in the LS. However, the vesicular transporters for acetylcholine and GABA were not or only rarely co-expressed with VGLUT3.In this study, the morphology and distribution of these VGLUT3-ir structures were systematically analyzed revealing that (1) they form distinct pericellular baskets (PBs) displaying variable shapes, (2) they are arranged in a layer-like pattern similar to the terminals of other modulatory systems, (3) beside a few exceptions (e.g., choline acetyltransferase), they are generally not or very sparsely colocalized with other neurochemical markers characterizing major neuron populations or afferent systems of the LS, i.e. calcium-binding proteins, tyrosine hydroxylase, tryptophan hydroxylase, vesicular glutamate transporters 1 (VGLUT1) and 2 (VGLUT2) and the vesicular GABA transporter.Thus, in the LS, a separate population of neurons is covered by VGLUT3-ir PBs. The distribution pattern and the lack of co-localization indicate that the VGLUT3-expressing cells of origin are located in the brainstem and that they could be pure glutamatergic projection neurons-different from the well-defined canonical VGLUT1-and VGLUT2-expressing neurons. Alternatively, they could

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Section: Characterization Of Antibodiesmentioning

confidence: 99%

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Riedel

Westerholz

Braun

et al. 2008

Journal of Chemical Neuroanatomy

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“…After ISH, the sections were rinsed briefly in PBS, treated with 0.2% Triton X-100 in PBS for 30 min at RT, and then preincubated for blocking in PBS containing 2.5% normal goat serum (NGS) for 1 hr at RT. They were incubated overnight at 4ЊC with two primary antibodies: one of anti-GluR1, 2/3, and 4 antibodies (2 g/ml, rabbit anti-glutamate receptor 1, 2/3, 4 polyclonal antisera; Chemicon International, Temecula, CA) (Petralia and Wenthold, 1992;, and one of anti-parvalbumin antibody (diluted 1:1000, monoclonal anti-parvalbumin mouse ascites fluid; Sigma, St. Louis, MO) (Celio et al, 1988) and anti-calbindin-D28k antibody (diluted 1:200, monoclonal anti-calbindin-D mouse ascites fluid; Sigma) . On the next day, the sections were washed three times for 5 min each time in PBS, treated in biotinylated anti-mouse IgG (diluted 1:200, second antibody for parvalbumin and calbindin-D28k; Vector Laboratories, Burlin- game, CA) for 1 hr at RT, and washed three times for 10 min each again.…”

Section: Methodsmentioning

confidence: 99%

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

1997

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The functional properties of AMPA-type glutamate receptors are determined by their subunit composition. We detected the expression of the AMPA receptor subunits (GluR1-GluR4) in neurons in the somatosensory cortex of adult rats by combining nonradioactive in situ hybridization using digoxigenin-labeled RNA probes of GluR1 and GluR2 with immunocytochemistry using specific antibodies against GluR1, GluR2/3, and GluR4. On the basis of differential expression of the GluR1 and GluR2 subunits, we classified the cortical neurons into four categories. To correlate the differential expression of AMPA receptor subunits in each neuron with that of two calcium-binding proteins, parvalbumin and calbindin-D28k, we used a triple-labeling method. The majority of cortical neurons (ϳ2/3) showed expression of GluR2 and undetectable expression of GluR1. GluR1-/GluR2-expressing neurons and GluR1-expressing/GluR2-undetectable neurons comprised ϳ1/10 each. Regarding the morphology, most GluR1-undetectable/GluR2-expressing neurons were pyramidal cells in layers II/III, V, and VI, whereas most GluR1-expressing/GluR2-undetectable neurons were nonpyramidal cells in layers II-VI. The GluR1-/GluR2-expressing neurons were either pyramidal or nonpyramidal. The majority of GluR1-/GluR2-expressing nonpyramidal cells was intensely stained with monoclonal antibody against calbindin-D28k, and one-half of the GluR1-undetectable/GluR2-expressing pyramidal neurons in layer II/III were lightly stained with this antibody. Most of GluR1-expressing/GluR2-undetectable neurons possessed parvalbumin immunoreactivity. These results indicate that neurons in the rat somatosensory cortex express differential combinations of GluR subunits, which correlate with the specific expression of the calcium-binding proteins.

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“…The following antisera were used for immunohistochemical staining (dilution in parentheses): (1) CaMKIV (1:500), a rabbit polyclonal antiserum raise against a recombinant protein corresponding to amino acids 328 -474 mapping to the C-terminal end of human CaMKIV (Santa Cruz Biotechnology, Santa Cruz, CA); (2) NeuN (1:1000), a mouse monoclonal antiserum (Chemicon, Temecula, CA); (3) Zif268 (1:10,000), a rabbit polyclonal antiserum (a gift from R. Bravo, BristolMyers Squibb, Princeton, NJ) that has been characterized previously (Herdegen et al, 1991); (4) SMI-32 (1:5000), a mouse monoclonal antiserum (Sternberger Monoclonals, Baltimore, MD) that has been characterized previously (Sternberger and Sternberger, 1983); (5) GABA (1: 200), a rabbit polyclonal antiserum (Chemicon) that has been characterized previously (Seguela et al, 1984); (6) GABA (1:1000), a guinea pig polyclonal antiserum (Chemicon); (7) parvalbumin (1:1000), a mouse monoclonal antiserum (Swant, Bellinzona, Switzerland) that has been characterized previously (Celio et al, 1988); (8) calbindin D-28K…”

Section: Methodsmentioning

confidence: 99%

Monocular Enucleation Induces Nuclear Localization of Calcium/Calmodulin-Dependent Protein Kinase IV in Cortical Interneurons of Adult Monkey Area V1

Lalonde¹,

Lachance²,

Chaudhuri³

2004

J. Neurosci.

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Elevation of intracellular Ca2ϩ levels activates calcium/calmodulin-dependent protein kinase (CaMK) IV, which in turn plays an important role in neuroprotection and neuroplasticity. The possibility that CaMKIV is similarly involved in neocortical tissue has not been examined previously, especially with regard to the plastic nature of ocular dominance features in the primary visual cortex (area V1). We addressed this question by way of monocular enucleation (ME) to disrupt sensory input and examine CaMKIV expression changes in monkey area V1. Immunohistochemical staining of area V1 in normal infants showed a nuclear presence of CaMKIV, which did not changed after ME. However, a striking set of layer-and time-dependent changes in nuclear CaMKIV expression was observed in adult area V1 after ME. A strong increase in nuclear CaMKIV levels was evident in cortical layers II/III and VI after 1 d of ME and in layer IVC after 5 d of ME. These specific laminar changes persisted after 30 d of ME and, most notably, showed a columnar profile in which CaMKIV expression was linked to open-eye columns. Real-time quantitative reverse transcription-PCR and Western blot analysis showed that total amounts of CaMKIV mRNA and protein remained unchanged after ME, suggesting that a nuclear translocation may occur from the cytoplasm. Finally, double-label immunohistochemical staining with a pyramidal cell marker (SMI-32) showed that CaMKIV was absent in this subtype, whereas coincidental expression with GABA, parvalbumin, and calretinin, but not calbindin, showed its clear presence in a subset of interneurons. We propose that CaMKIV activity within diverse groups of cortical interneurons may play an important role in adaptive plastic reorganization of adult neocortical tissue.

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Monoclonal antibodies directed against the calcium binding protein parvalbumin

Cited by 383 publications

References 11 publications

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Vesicular glutamate transporter 3-immunoreactive pericellular baskets ensheath a distinct population of neurons in the lateral septum

Combinations of AMPA Receptor Subunit Expression in Individual Cortical Neurons Correlate with Expression of Specific Calcium-Binding Proteins

Monocular Enucleation Induces Nuclear Localization of Calcium/Calmodulin-Dependent Protein Kinase IV in Cortical Interneurons of Adult Monkey Area V1

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