Monoclonal antibodies directed against plasmid-encoded released proteins of enteropathogenic Yersinia

Heesemann, J

doi:10.1016/0378-1097(86)90122-9

Cited by 5 publications

(7 citation statements)

References 6 publications

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“…Our results support and extend data given in a previous report [9], which was based on a different collection of Yersinia strains, namely, Y. enterocolitica enterocolitica bacteria was observed.…”

Section: Serological Relationship Between Omps Of Different Yersinia supporting

confidence: 95%

“…The virulence [4] and a number of temperature-inducible features of these bacteria, such as the production of V and W antigens [5], Ca++-dependent growth at 37~ in vitro [5,7], adherence to Hep-2 cells [15] and autoagglutination [ 12] have been associated with the presence of a 40-46 Mdal plasmid. This plasmid codes also for the temperature-dependent expression of at least five outer membrane proteins (OMPs) of molecular mass of approximately 200 kd, 47 kd, 41 kd, 34-36 kd and 25 kd [2,19] which show related antigenic features as documented by immunoblot analysis using hyperimmune sera [9]. The plasmidencoded OMPs expressed by Y. enterocolitica are similar in size when compared with those expressed by Y. pseudotuberculosis [ 1,19].…”

Section: Introductionmentioning

confidence: 98%

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Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Weninger

Schoerner

Wartenberg

et al. 1989

Med Microbiol Immunol

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Plasmid-positive Yersinia bacteria of different species and different serotypes were analysed with respect to the immunological relationship of two of their plasmid-encoded outer membrane proteins (OMPs) by the immunoblot technique using three monoclonal antibodies (mAb), which were induced against OMPs of Yersinia enterocolitica serotype O:9 bacteria. Evidence is presented that these OMPs display discrete epitopes, which are common to all plasmid-positive Yersinia strains tested, with only one exception, indicating a close structural relationship of the OMPs analysed.

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Section: Serological Relationship Between Omps Of Different Yersinia supporting

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Weninger

Schoerner

Wartenberg

et al. 1989

Med Microbiol Immunol

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“…Although Yops of all three pathogenic Yersinia spp. are closely related, they can vary in size (39,41). YopM (ranging from 49 kDa to 57 kDa) exhibits significant homology with several domains of the human platelet glycoprotein GPIba (59,73).…”

Section: Pathogenic Factors Of Yersinia Enterocoliticamentioning

confidence: 99%

Experimental Yersinia enterocolitica infection in rodents: A model for human yersiniosis

Heesemann

Gaede

Autenrieth

1993

APMIS

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enterocolitica infection in rodents: A model for human yersiniosis. APMIS 101: 417429, 1993. Yersiniu enterocoliticu infection in humans causes a broad spectrum of diseases ranging from acute bowel disease to extraintestinal manifestations such as reactive arthritis, erythema nodosum and uveitis. During the last decade a fascinating part of the molecular biology of the pathogenicity of human pathogenic Yersinia species has been unraveled. Pathogenicity factors such as protein tyrosine phosphatase, protein kinase, thrombin-and collagen-binding factors have been identified and characterized on the molecular level. In contrast to many animal models for human enteropathogenic microorganisms, experimental Y enterocoliticu infection in rodents resembles yersiniosis in humans and thus offers extraordinary opportunities to study the sequential steps of the infectious process. Rabbits are suitable animals in which to study Yersinia-induced enteritis (enterotoxin-mediated) and the humoral immune response after oral infection. The role of Peyer's patches (PP) in the entry of enteropathogenic Yersinia species has been elucidated in mice and rabbits. M cells are probably the primary target cells of invading Yersiniae. Surprisingly, after penetration of the mucosal epithelial cell layer Yersinia bacilli were visualized to be exclusively extracellular in PP tissue. Obviously neutrophils within PP were unable to phagocytize the invading microorganisms. Presently, it is not clear how the microorganisms disseminate from PP into lymph nodes, spleen, liver and lung of mice where they form abscesses and granuloma-like lesions. Immunohistologically the involvement of macrophages and T cells could be demonstrated in Yersinia-induced lesions of mice. Direct evidence for the role of T cells and cytokine-activated macrophages in the host defense reaction against a primary Yersinia infection in mice could be obtained from experiments including adoptive transfer of Yersinia-specific T cells and in vivo neutralization of TNF-alpha and IFN-gamma. The experimental rat model turned out to be a suitable model for studying Yersinia-induced aseptic arthritis. Lewisand SHR rats proved to be arthritis-susceptible. Arthritogenicity of Yersinia for rats appeared to be restricted to I: enterocoliticu of serotype 08 and correlated with the virulence potential of this serotype. Surprisingly, expression of YadA, the collagen-binding factor, was not necessary for arthritis induction. A close association between both susceptibility to arthritis induction and Yersinia infection could be demonstrated in various rat strains. Depletion of alpha/beta T-cell receptor (crp-TCR)-positive T cells by treatment with ap-TCR-specific antibody revealed that T cells were required for clearance of the pathogen. However, in contrast to e.g. adjuvant arthritis, Yersinia-induced arthritis in Lewis rats was not affected by T-cell depletion. Thus, we expect significant differences between the pathomechanisms of Yersinia-triggered arthritis and antigen-induced arthritis in Le...

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“…Following three booster immunizations, splenocytes of these * Corresponding author. mice were fused with Ag 8.653 myeloma cells and resulting hybridomas were screened for reactivity with tachyzoites and bradyzoites by immunoblotting and immunofluorescence (6). Tachyzoites for all experiments were harvested from infected P388D1-macrophage cell cultures as described previously (5).…”

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confidence: 99%

“…Cysts were separated from brains of mice, infected intraperitoneally with 103 tachyzoites 6 weeks before, by continuous density gradient centrifugation as previously described (4). For immunoblotting, lysates of 3 x 106 tachyzoites per well or 200 cysts per well were separated on 11.8% acrylamide gels by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (6,9). Following electrophoresis, proteins were electrophoretically transferred onto 0.45-pm-pore-size nitrocellulose membranes.…”

mentioning

confidence: 99%

Induction of bradyzoite-specific Toxoplasma gondii antigens in gamma interferon-treated mouse macrophages

1993

Self Cite

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By using stage-specific monoclonal antibodies, an in vitro model has been developed to analyze the kinetics of expression of stage-specific antigens during the conversion process between tachyzoites and bradyzoites of Toxoplasma gondii. Following infection of murine macrophages with bradyzoites, the expression of bradyzoitespecific antigens declined, whereas the expression of tachyzoite-specific antigens increased during the first 72 h postinfection. Conversely, in gamma interferon-treated murine macrophages infected with tachyzoites, the inhibitory effect of gamma interferon on replication of parasites was accompanied by the induction of bradyzoite-specific antigens.

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Monoclonal antibodies directed against plasmid-encoded released proteins of enteropathogenic Yersinia

Cited by 5 publications

References 6 publications

Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Detection of cross-reacting epitopes on plasmid-encoded outer membrane proteins of enteropathogenic Yersinia by monoclonal antibodies

Experimental Yersinia enterocolitica infection in rodents: A model for human yersiniosis

Induction of bradyzoite-specific Toxoplasma gondii antigens in gamma interferon-treated mouse macrophages

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