Monoclonal antibodies directed against human Rh antigens in tests with the red cells of nonhuman primates

Socha, W. W.; Ruffié, J

doi:10.1016/s1140-4639(05)80004-8

Cited by 20 publications

(15 citation statements)

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“…The deduced amino acid sequence indicated that the monkey Rh-like proteins exhibit 75-78% similarity to the human Rh proteins. The Rh-like proteins presumably inserted in the red cell membrane would account for the reactivity of the M. mulatta red blood cells with two monoclonal antibodies (anti-Rh29) that recognize nonpolymorphic epitopes at the red cell surface of human and nonhuman primates (Socha and Ruffi6 1990). Furthermore, these polypeptides may correspond to the Rh analog protein of 32 kDa isolated from rhesus monkey erythrocyte membranes (Saboori et al 1989).…”

Section: Discussionmentioning

confidence: 99%

“…do not. Human anti-c antibodies agglutinate gibbon, chimpanzee, orangutan, and gorilla red cells whereas human anti-D react only with chimpanzee, orangutan, and gorilla cells (Wiener et al 1964;Shaw 1986;Socha and Ruffi6 1990). Analogs of nonhuman Rh proteins have also been described recently in monkey, cow, cat, and rat as 32-kDa erythrocyte polypeptides (Saboori et al 1989).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of the rh-like locus and gene transcripts from the rhesus monkey (Macaca mulatta)

et al. 1994

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The human Rh blood group locus consists of two structurally related genes (D and CcEe) in Rh-positive haplotypes but a single gene (CcEe) in Rh-negative haplotypes. The genome of rhesus monkeys (Macaca mulatta), while not expressing any of the human Rh D, C, c, E, or e specificities, carries a Rh-like locus strongly related to the human Rh locus. Southern blot analysis suggested the presence of only one Rh-like gene with an additional truncated fragment corresponding to the 5' region. RNA preparations from M. mulatta bone marrow cells contained Rh-like species of 1.7 kb. Two allelic Rh-like transcripts were amplified by PCR and sequenced. The predicted translation product of the first transcript was a 417-amino-acid protein closely similar to the human Rh counterpart. The predicted product of the second transcript consisted of a 361-amino-acid polypeptide truncated in the NH2 terminal region and differing from the former by a few substitutions. The macaque Rh-like protein sequences differed from those of human D and Cc/Ee polypeptides by 22-25%, whereas the degree of identity between the human proteins was 91.5%. Implications of these results in the analysis of the evolutionary pathway of the Rh locus are discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the rh-like locus and gene transcripts from the rhesus monkey (Macaca mulatta)

et al. 1994

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“…As previouly reported, chimpanzees' red cells display antigens closely related to human D and, therefore, may serve as another reference system for defining minute differences between monoclonal anti-D reagents [2],…”

Section: Introductionmentioning

confidence: 90%

“…(6) Tests with human mAbs were carried out, as previously de scribed [2], by three standard hemagglutination methods: direct ag glutination of untreated cells, and ficin-treated red cells (enzymetreated cell method, ETC) and antiglobulin (indirect antiglobulin test. IAT) (using rabbit antihuman IgG serum).…”

Section: Introductionmentioning

confidence: 99%

Diversity of Human Anti-D Monoclonal Antibodies Revealed by Reactions with Chimpanzee Red Blood Cells

Blancher¹,

Socha²,

Ruffie³

1992

Vox Sanguinis

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Fifty-three human anti-D monoclonal antibodies (mAbs) revealed a striking diversity of reactions in tests with panels of chimpanzee red blood cells (RBCs) of various R-C-E-F blood group phenotypes (counterparts of the human Rh- Hr groups). The reactivities of these antibodies, which depended on the agglutination technique used, could be classified into four main types. These patterns of reactivity of anti-D mAbs with chimpanzee RBCs showed only limited correlation with types of reactions observed with human D variant RBCs. Primate red cells may, therefore, constitute an independent test system for subclassification of human monoclonal antibodies. Comparison of reactivities of human anti-D mAbs with chimpanzee and human D variant RBCs confirms the homology between the chimpanzee Rc, and the human D antigens. The chimpanzee Rc shares with human D the epitopes epD5, epD6/7 and epD8, but lacks epitopes epD1, epD2, epD3 and epD4 of the Rh mosaic, thus resembling the human D variants IVb and Vc.

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“…This was reflected in survey articles, in that many of the reviewed mAb articles dealt with basic-type mAb science, such as comparing antigenic properties of red and white blood cells (Neubauer et al, 1981;Socha and Ruffie, 1990). While many studies found cross-reactivity with human and chimpanzee epitopes, such findings were by no means uniform, and a number of studies confirmed differences between humans and chimpanzees (Hammond and Robinson, 1984;Palmer et al, 1987;lu et al, 1987).…”

Section: Topics Of Researchmentioning

confidence: 99%

Systematic review of chimpanzee use in monoclonal antibody research and drug development: 1981-2010

Bettauer

2011

ALTEX

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Monoclonal antibodies directed against human Rh antigens in tests with the red cells of nonhuman primates

Cited by 20 publications

References 3 publications

Molecular characterization of the rh-like locus and gene transcripts from the rhesus monkey (Macaca mulatta)

Molecular characterization of the rh-like locus and gene transcripts from the rhesus monkey (Macaca mulatta)

Diversity of Human Anti-D Monoclonal Antibodies Revealed by Reactions with Chimpanzee Red Blood Cells

Systematic review of chimpanzee use in monoclonal antibody research and drug development: 1981-2010

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