Monoclonal antibodies as tools to define receptors for C3b, C3bi and C3d on human lymphocytes, phagocytes, erthyrocytes and renal glomerulum cells

Dierich, M. P.; Scheiner, Otto; Mussel, H.-H.; Schmitt, Michèle; Burger, Roger A.; Ehlen, Thomas; Peters, Helmut

doi:10.1016/0161-5890(82)90042-6

Cited by 1 publication

(6 citation statements)

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“…A similar effect was observed with culture supernatant of clone 298-D10 as published in a recent paper [19] where possible ex planations for this finding are discussed. Figure 6 shows the influence of DFP on the rosette formation of Raji cells with C3b-, iC3b-, and C3d-DFF; mM lymphocytes and C3b, C3bi, and C3d cells, respec tively, during rosette formation revealed no effect on the rosette formation with these intermediates (fig.…”

Section: Reactivity O F Raji Cells With Monoclonal Anti-c3hsupporting

confidence: 72%

“…Therefore, we concluded that Raji cells do indeed bind to EACI4°23b via their C'3b receptors. Furthermore, monoclonal antibodies react ing exclusively with C3b receptors [19] on human lymphoid, erythroid, and renal cells interfered with the rosette formation of Raji cells with EACI4°23b ( fig. 4).…”

Section: Discussionmentioning

confidence: 99%

“…Monoclonal antibodies directed against C3b receptors were prepared as described by Dierich et al [19]. Clone 298DI0 was selected for the experiments described in this paper.…”

Section: Methodsmentioning

confidence: 99%

“…The cells were washed twice in VBS-EDTA of the same temperature and C3b receptor ac tivity was assessed as described above. Inhibition of rosette forma tion of EACI4°23b with Raji cells with monoclonal antibodies was performed as described previously [19], with the exception that as cites fluid was used.…”

Section: Methodsmentioning

confidence: 99%

“…Receptor A ntibodies Raji cells preincubated with monoclonal antibod ies (clone 298-D10) [19] in different concentrations exhibited a dose-dependent inhibition of the capacity to form rosettes with EACI4°23b ( fig. 5).…”

Section: Reactivity O F Raji Cells With Monoclonal Anti-c3hmentioning

confidence: 99%

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A Comparative Evaluation of Receptor Reactivities for C3b, iC3b, and C3d on Raji Lymphoblastoid Cells

Scheiner¹,

Hammann²,

Schmid³

et al. 1982

Int Arch Allergy Immunol

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Raji cells were described to carry receptors for iC3b, C3d, C3b-β1H and β1H. Controversial opinions, however, exist whether or not these cells carry also receptors for C3b. Using highly purified C3, definitely devoid of β1H and C5, for preparation of C3b intermediates, it could be shown that Raji cells bound to C3b cells. Furthermore, Raji cells reacted with monoclonal antibodies that interfered with binding of C3b to human erythrocytes, lymphocytes and renal cells. The receptor for C3b on Raji cell, however, exhibited some special properties and, therefore, required some distinct experimental conditions for its detection: (1) The origin of the erythrocytes used for preparation of the C3b intermediates seemed to be important; this was not the case when iC3b and C3d receptor reactivity was assessed. (2) Rosettes already formed between Raji cells and EAC 1423b showed the tendency to disintegrate within the first 30 min after the rosette formation assay. Again, this effect could not be observed with iC3b- and C3d-dependent rosette formation. (3) Incubation of the Raji cells at 37 °C as well as at 4°C before rosette formation resulted in a rhythmic loss and reappearance of C3b receptor reactivity. At room temperature (19–22°C) this effect was much less expressed. There was no influence of preincubation at 4 and 37 °C, respectively, on the iC3b and C3d receptor reactivity of Raji cells. (4) Diisopropylfluorophosphate (DFP) present during rosette formation enhanced, within a certain range of concentration, the percentage of C3b-dependent rosette formation. iC3b and C3d receptor reactivity was not influenced. A similar reaction pattern was observed with pokeweed mitogen (PWM)-stimulated tonsil lymphocytes. In the concentrations tested, DFP showed no effect on the rosette formation between C3b, iC3b, and C3d cells, respectively, and unstimulated tonsil lymphocytes.The data presented suggest that C3b receptors on Raji cells undergo some special metabolism, possibly controlled by fluid phase or cell-bound proteases. This might be a common property of C3b receptors on blast-like and transformed cells, differing from that of unstimulated small lymphocytes.

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Section: Reactivity O F Raji Cells With Monoclonal Anti-c3hsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

“…Monoclonal antibodies directed against C3b receptors were prepared as described by Dierich et al [19]. Clone 298DI0 was selected for the experiments described in this paper.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Reactivity O F Raji Cells With Monoclonal Anti-c3hmentioning

confidence: 99%

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