Monoclonal Antibodies as Probes for the Analysis of the Secretory Ependymal Differentiation in the Subcommissural Organ of the Chick Embryo

Didier, Robert; Meiniel, R; Meiniel, Annie

doi:10.1159/000111646

Cited by 26 publications

(12 citation statements)

References 9 publications

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“…As previously reported, the chick SCO starts to synthesize AFRU-immunoreactive secretory material around E2.5-E3 (Schoebitz et al 1986;Didier et al 1992Didier et al , 2007. Use of either the polyclonal or the monoclonal antibody revealed immunoreactive SCO cells in prosomere 1 at E3 (Fig.…”

Section: Sco Differentiation and Pc Development In Early Chick Embryossupporting

confidence: 76%

“…Several lines of evidence support such a relationship. First, in the chick, the SCO develops early, between E2.5 and E3 (Schoebitz et al 1986;Didier et al 1992Didier et al , 2007, and PC pioneering axons start to cross the midline at E3 (Figdor and Stern 1993;Chedotal et al 1995). Second, the SCO is located in the dorsal midline of the brain, the midline of the developing brain being an important "choice point" or "intermediate target" for pathfinding axons (Kaprielian et al 2000Dickson 2002;Williams et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Various lines of evidence suggest the involvement of the SCO in PC formation: (1) in the chick, SCO differentiation occurs at E2.5-E3 (Schoebitz et al 1986;Didier et al 1992Didier et al , 2007 concomitantly with the crossing of pioneering axons of the PC (Figdor and Stern 1993;Chedotal et al 1995), although such temporal coincidence does not occur in rodent species (Schoebitz et al 1993); (2) the SCO is located in the dorsal midline and the role of this midline as an intermediate target in the axonal pathway is widely accepted (Kaprielian et al 2000Dickson 2002;Williams et al 2004); (3) data from various null mutant mice indicate that animals lacking SCO or with SCO alterations fail to form a normal PC (Louvi and Wassef 2000;Estivill-Torrus et al 2001;Fernandez-Llebrez et al 2004;Ramos et al 2004); (4) SCO-spondin belongs to the thrombospondin superfamily, sharing type 2 TSR domains with molecules implicated in axonal pathfinding during ependymalnervous system development, such as Rspondin, F-spondin, mindin and semaphorins (Feinstein and Klar 2004;Kamata et al 2004;Meiniel and Meiniel 2007);(5) in vitro experiments on SCO-conditioned medium, RF solubilized compounds or synthetic peptides derived from SCO-spondin have shown, in various cell culture systems, the potential role of SCO-spondin in neuronal survival and aggregation and in neurite outgrowth (Monnerie et al , 1997(Monnerie et al , 1998El-Bitar et al 2001); (5) SCO-spondin is mainly expressed by two midline structures, viz. the SCO (Creveaux et al 1998;Gobron et al 2000) and the rostral floor plate, albeit only during certain stages of embryonic development (Lichtenfeld et al 1999;Richter et al 2001;Guiñazú et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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The subcommissural organ and the development of the posterior commissure in chick embryos

et al. 2009

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The subcommissural organ (SCO) is an ependymal differentiation located in the diencephalon under the posterior commissure (PC). SCO-spondin, a glycoprotein released by the SCO, belongs to the thrombospondin superfamily and shares molecular domains with axonal pathfinding molecules. Several lines of evidence suggest a relationship between the SCO and the development of the PC in the chick: (1) their close location to each other, (2) their differentiation at the same developmental stage in the chick, (3) the abnormal PC found in null mutants lacking an SCO and (4) the release by the SCO of SCO-spondin. By application of DiI crystals in the PC of chick embryos, we have identified the neurons that give rise to the PC. Labelling is confined to the magnocellular nucleus of the PC (MNPC). To gain insight into the role of the SCO in PC development, coculture experiments of explants of the MNPC region (MNPCr) from embryos at embryonic day 4 (E4) with SCO explants from E4 or E13 embryos have been performed and the neurite outgrowth from the MNPCr explants has been analysed. In the case of coculture of E4 MNPCr with E4 SCO, the number of neurites growing from the MNPCr is higher at the side facing the SCO. However, when E4 MNPCr and E13 SCO are cocultured, the neurites grow mostly at the side opposite to the SCO. These data suggest that, at early stages of development, the SCO releases some attractive or permissive molecule(s) for the growing of the PC, whereas at later stages, the SCO has a repulsive effect over neurites arising from MNPCr.

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Section: Sco Differentiation and Pc Development In Early Chick Embryossupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The subcommissural organ and the development of the posterior commissure in chick embryos

et al. 2009

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“…Monoclonal antibodies against the secretory products of the SCO have previously been obtained by using crude extracts of the SCO of bovine (Meiniel et al 1988) or chick embryos (Didier et al 1992) as the immunogen; these extracts contain the precursor and processed forms of the secretory compounds. The present investigation reports the production of monoclonal antibodies against the constituent glycoproteins of the bovine RF, that is the processed forms of the material secreted by the SCO.…”

Section: Discussionmentioning

confidence: 99%

Bovine Reissner's fiber (RF) and the central canal of the spinal cord: an immunocytochemical study using a set of monoclonal antibodies against the RF-glycoproteins

Pérez

Garrido

Cifuentes

et al. 1996

Cell and Tissue Research

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The subcommissural organ secretes N-linked complex-type glycoproteins into the cerebrospinal fluid. These glycoproteins condense to form Reissner's fiber (RF), which extends along the fourth ventricle and central canal of the spinal cord. A set of three monoclonal antibodies (Mabs 3E6, 3B1, and 2A5) has been obtained using these glycoproteins as immunogens. Competitive and sandwich enzyme-linked immunoassay methods have demonstrated that the three monoclonal antibodies are directed against different epitopes, and that there is no competition among them for their binding to glycoproteins of RF. Mab 3E6 displays immunoblotting properties that are similar to those of a polyclonal antibody against the pool of glycoproteins from RF, but that are different from those of Mabs 3B1 and 2A5. All three antibodies immunostain the bovine subcommissural organ and RF. A population of ependymal cells is stained by the polyclonal antibody, and Mabs 2A5 and 3E6, but not by Mab 3B1. The material present in a population of ependymal cells of the central canal, and the glycoproteins secreted by the subcommissural organ thus probably have partial chemical identity. Some evidence suggests that the immunoreactive ependymal cells are secretory cells. The luminal surface of the central canal is coated by a thin layer of material with immunocytochemical characteristics different from those of the ependymal cells; such a coat may correspond to material released from RF.

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“…The SCO/RF complex is consistently found in the brain of all chordate classes (Oksche et al 1993;Olsson 1993) and appears very early during ontogenesis (Didier et al 1992). The complex-type nature of these secreted glycoproteins has been established by the use of lectin histochemistry (Rodríguez et al 1986;Meiniel et al 1988b;Herrera et al 1990).…”

Section: Introductionmentioning

confidence: 96%

Reissner's fibre promotes neuronal aggregation and influences neuritic outgrowth in vitro

Monnerie

Dastugue

Meiniel

1997

Cell and Tissue Research

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Reissner's fibre is the condensed form of glycoproteins secreted by the subcommissural organ; it extends through the central canal to the caudal end of the spinal cord. The effect of Reissner's fibre was assessed on dissociated embryonic chick cortical neuronal cells grown in chemically defined medium, by using two cell culture systems: (1) low-density cultures, in which neuronal cells remained evenly distributed; (2) high-density cultures, in which neuronal cells aggregated, displaying prominent neuritic outgrowth. Reissner's fibre, when added to low-density cultures, induced neuronal aggregation and neuritic outgrowth but this effect was restricted to an area centred around Reissner's fibre. Reissner's fibre, when added to high-density cultures, potentiated cell aggregation. Antibodies inhibiting the neural cell adhesion molecule or N-cadherin, and soluble Reissner's fibre material (reported previously to have anti-aggregative activity), did not prevent the aggregative activity induced by Reissner's fibre. Neuronal cells showed a similar reaction pattern when heparin or Reissner's fibre was added to the culture. These results suggest that the subcommissural organ/Reissner's fibre complex has multifunctional activities and may modulate cell-cell interactions during the development of the central nervous system.

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Monoclonal Antibodies as Probes for the Analysis of the Secretory Ependymal Differentiation in the Subcommissural Organ of the Chick Embryo

Cited by 26 publications

References 9 publications

The subcommissural organ and the development of the posterior commissure in chick embryos

The subcommissural organ and the development of the posterior commissure in chick embryos

Bovine Reissner's fiber (RF) and the central canal of the spinal cord: an immunocytochemical study using a set of monoclonal antibodies against the RF-glycoproteins

Reissner's fibre promotes neuronal aggregation and influences neuritic outgrowth in vitro

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