Monoclonal Antibodies against Pectin

Liners, Françoise; Letesson, Jean‐Jacques; Didembourg, Christian; Cutsem, Pierre Van

doi:10.1104/pp.91.4.1419

Cited by 206 publications

(82 citation statements)

References 18 publications

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“…The specificities of the primary antibodies JIM5 and JIM7 (CarboSource) have been extensively described (Liners et al, 1989;Knox et al, 1990;Knox, 1997;Willats et al, 2001aWillats et al, , 2001bWillats et al, , 2001cMacquet et al, 2007a;Pattathil et al, 2010;Xu et al, 2011). JIM13, MAC207, JIM8, and CCRC-M7 (CarboSource) have also been previously described (Bradley et al, 1988;Pennell et al, 1989Pennell et al, , 1991Knox et al, 1991;Puhlmann et al, 1994;Yates and Knox, 1994;Steffan et al, 1995;Yates et al, 1996;Majewska-Sawka and Nothnagel, 2000;Pattathil et al, 2010).…”

Section: Microscopy and Image Analysismentioning

confidence: 99%

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

Griffiths¹,

Tsai²,

Xue

et al. 2014

Plant Physiology

135

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Interactions between cell wall polymers are critical for establishing cell wall integrity and cell-cell adhesion. Here, we exploit the Arabidopsis (Arabidopsis thaliana) seed coat mucilage system to examine cell wall polymer interactions. On hydration, seeds release an adherent mucilage layer strongly attached to the seed in addition to a nonadherent layer that can be removed by gentle agitation. Rhamnogalacturonan I (RG I) is the primary component of adherent mucilage, with homogalacturonan, cellulose, and xyloglucan constituting minor components. Adherent mucilage contains rays composed of cellulose and pectin that extend above the center of each epidermal cell. CELLULOSE SYNTHASE5 (CESA5) and the arabinogalactan protein SALT-OVERLY SENSITIVE5 (SOS5) are required for mucilage adherence through unknown mechanisms. SOS5 has been suggested to mediate adherence by influencing cellulose biosynthesis. We, therefore, investigated the relationship between SOS5 and CESA5. cesa5-1 seeds show reduced cellulose, RG I, and ray size in adherent mucilage. In contrast, sos5-2 seeds have wild-type levels of cellulose but completely lack adherent RG I and rays. Thus, relative to each other, cesa5-1 has a greater effect on cellulose, whereas sos5-2 mainly affects pectin. The double mutant cesa5-1 sos5-2 has a much more severe loss of mucilage adherence, suggesting that SOS5 and CESA5 function independently. Doublemutant analyses with mutations in MUCILAGE MODIFIED2 and FLYING SAUCER1 that reduce mucilage release through pectin modification suggest that only SOS5 influences pectin-mediated adherence. Together, these findings suggest that SOS5 mediates adherence through pectins and does so independently of but in concert with cellulose synthesized by CESA5.

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Section: Microscopy and Image Analysismentioning

confidence: 99%

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

Griffiths¹,

Tsai²,

Xue

et al. 2014

Plant Physiology

135

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“…Immunolabelling of demethylesterified HG was conducted on transverse sections of meristems using 2F4 antibodies (Liners et al, 1989). All immunolabelling experiments were carried out using a buffer containing 0.5 mM CaCl 2 and milk as previously described (Peaucelle et al, 2008).…”

Section: Immunolabelling Of Pectinsmentioning

confidence: 99%

The transcription factor BELLRINGER modulates phyllotaxis by regulating the expression of a pectin methylesterase in Arabidopsis

et al. 2011

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SUMMARYPlant leaves and flowers are positioned along the stem in a regular pattern. This pattern, which is referred to as phyllotaxis, is generated through the precise emergence of lateral organs and is controlled by gradients of the plant hormone auxin. This pattern is actively maintained during stem growth through controlled cell proliferation and elongation. The formation of new organs is known to depend on changes in cell wall chemistry, in particular the demethylesterification of homogalacturonans, one of the main pectic components. Here we report a dual function for the homeodomain transcription factor BELLRINGER (BLR) in the establishment and maintenance of the phyllotactic pattern in Arabidopsis. BLR is required for the establishment of normal phyllotaxis through the exclusion of pectin methylesterase PME5 expression from the meristem dome and for the maintenance of phyllotaxis through the activation of PME5 in the elongating stem. These results provide new insights into the role of pectin demethylesterification in organ initiation and cell elongation and identify an important component of the regulation mechanism involved.

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“…First, certain components of pectin are localized appropriately to play this role. Monoclonal antibodies that recognize specific regions of pectin have been used to visualize pectin immunocytochemically (Knox et al, 1990;Liners et al, 1989;Puhlmann et al, 1994;Williams et al, 1996). Unesterified pectin is localized to the expanded region of the middle lamella proximal to the intercellular space, and to the surface of the wall at the intercellular space in carrot roots (Knox et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall

1998

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SummaryThe quartet (qrt) mutants of Arabidopsis thaliana produce tetrad pollen in which microspores fail to separate during pollen development. Because the amount of callose deposition between microspores is correlated with tetrad pollen formation in other species, and because pectin is implicated as playing a role in cell adhesion, these cell-wall components in wild-type and mutant anthers were visualized by immunofluorescence microscopy at different stages of microsporogenesis. In wild-type, callose was detected around the pollen mother cell at the onset of meiosis and around the microspores during the tetrad stage. Microspores were released into the anther locule at the stage where callose was no longer detected. Deposition and degradation of callose during tetrad pollen formation in qrt1 and qrt2 mutants were indistinguishable from those in wild-type. Enzymatic removal of callose from wild-type microspores at the tetrad stage did not release the microspores, suggesting that callose removal is not sufficient to disperse the microspores in wild-type. Pectic components were detected in the primary wall of the pollen mother cell. This wall surrounded the callosic wall around the pollen mother cell and the microspores during the tetrad stage. In wild-type, pectic components of this wall were no longer detectable at the time of microspore release. However, in qrt1 and qrt2 mutants, pectic components of this wall persisted after callose degradation. This result suggests that failure of pectin degradation in the pollen mother cell wall is associated with tetrad pollen formation in qrt mutants, and indicates that QRT1 and QRT2 may be required for cell type-specific pectin degradation to separate microspores.

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Monoclonal Antibodies against Pectin

Cited by 206 publications

References 18 publications

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

SALT-OVERLY SENSITIVE5 Mediates Arabidopsis Seed Coat Mucilage Adherence and Organization through Pectins

The transcription factor BELLRINGER modulates phyllotaxis by regulating the expression of a pectin methylesterase in Arabidopsis

Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall

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