Monoclonal antibodies against Met-enkephalin as probe in the central nervous system

Pontarotti, Pierre; Kaouël, C. Le Borgne De; Verrier, M.; Cupo, A.

doi:10.1016/0165-5728(83)90063-2

Cited by 19 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it did not cross-react with P-endorphin or dynorphins. An additional anti-enkephalin antibody (clone B21; Pontarotti et al, 1983) was also used. It was raised against met-enkephalin and was reported to show no cross-reaction with leu-enkephalin, C-terminally extended enkephalins and with dynorphins.…”

Section: Primary Antibodiesmentioning

confidence: 99%

Choline Acetyltransferase, Glutamate Decarboxylase, Tyrosine Hydroxylase, Calcitonin Gene‐related Peptide and Opioid Peptides Coexist in Lateral Efferent Neurons of Rat and Guinea‐pig

Safieddine

Prior

Eybalin

1997

Eur J of Neuroscience

View full text Add to dashboard Cite

The lateral efferent (olivocochlear) innervation of the cochlea originates in the brainstem lateral superior olive. It is likely to use acetylcholine, gamma-aminobutyric acid, dopamine and various neuropeptides as neurotransmitters and/or neuromodulators. In order to determine the different coexistence patterns of these molecules in lateral efferent perikarya, we have used double and triple immunofluorescence co-localization techniques to colocalize choline acetyltransferase, glutamate decarboxylase, tyrosine hydroxylase, calcitonin gene-related peptide and enkephalins in single sections of the lateral superior olive. We also used a non-radioactive in situ hybridization technique onto serial sections of this nucleus to confirm the immunofluorescence co-localization data at the mRNA level. Whatever the pair or triplet of primary antibodies tested was, a high ratio of coexistence was observed in the immunofluorescence experiments. In triple co-localization experiments, 90-93% of the choline acetyltransferase-like immunoreactive neurons were also immunoreactive to the two other antigens investigated. The in situ hybridization co-localization data, based on the use of biotin-labelled oligoprobes, qualitatively confirmed these immunofluorescence data. In conclusion, it can be postulated that acetylcholine, gamma-aminobutyric acid, dopamine, calcitonin gene-related peptide, enkephalins and dynorphins (whose coexistence with choline acetyltransferase and enkephalins has been previously described immunocytochemically) coexist in lateral efferent neurons. Based on these results, it is tempting to propose the lateral efferent innervation as a useful model with which the functional implications of the coexistence of neurotransmitters/neuromodulators can be investigated in vivo.

show abstract

Section: Primary Antibodiesmentioning

confidence: 99%

Choline Acetyltransferase, Glutamate Decarboxylase, Tyrosine Hydroxylase, Calcitonin Gene‐related Peptide and Opioid Peptides Coexist in Lateral Efferent Neurons of Rat and Guinea‐pig

Safieddine

Prior

Eybalin

1997

Eur J of Neuroscience

View full text Add to dashboard Cite

show abstract

“…Immunohistochemistry was performed as described by Pruss et al (21,22) using mouse monoclonal anti-enkephalin (23) combined with the rabbit anti-porcine GAL used in the RIA (see below). Rhodamine-and fluorescein-coupled goat antiserum specific for mouse and rabbit immunoglobulins was purchased from Cappel (Durham, NC).…”

Section: Immunohistochemistrymentioning

confidence: 99%

Galanin Gene Expression in Chromaffin Cells Is Controlled by Calcium and Protein Kinase Signaling Pathways

1990

View full text Add to dashboard Cite

The neuropeptide galanin (GAL) is widely distributed throughout the diffuse neuroendocrine system, and is coexpressed with acetylcholine, norepinephrine, prolactin, and a variety of other messenger substances in different cell types. Bovine chromaffin cells in primary culture synthesize and store GAL along with catecholamines, chromogranin A, and enkephalin peptides, as well as other neurosecretory products, and secrete all these molecules in response to nicotinic stimulation. The regulation of GAL biosynthesis and secretion were studied by measuring changes in messenger RNA (mRNA(GAL], and peptide immunoreactivity, 24-72 h after stimulation of secretion (40 mM potassium or 10 microM veratridine), or exposure to stimulators of protein kinase C (100 nM phorbol myristate acetate) and protein kinase A (25 microM forskolin). Depolarization-induced stimulation of GAL biosynthesis, like that of enkephalin and other neuropeptides, was calcium dependent, suggesting that calcium generally mediates both exocytotic release and new peptide synthesis thus coordinating maintenance of neuropeptide levels in chromaffin cells. GAL and mRNA(GAL) were also upregulated by stimulation of protein kinase A with forskolin. Treatment with PMA increased GAL and mRNA(GAL) to an even greater extent than depolarization. Thus GAL expression can be regulated by three distinct signal transduction systems in chromaffin cells: depolarization-stimulated calcium influx, activation of protein kinase C and activation of protein kinase A, which in addition differentially up- and down-regulate the expression of several other neurosecretory proteins and peptides resulting in different patterns of GAL/neuropeptide coexistence in bovine chromaffin cells. GAL coexistence with diverse neuroendocrine substances may reflect the relative activity of these three signalling systems in other neuroendocrine cell types as well.

show abstract

From outer to inner space: Traveling along a scientific career from astro-chemistry to drug research

Glasel

2001

Progress in Drug Research 57

View full text Add to dashboard Cite

Monoclonal antibodies against Met-enkephalin as probe in the central nervous system

Cited by 19 publications

References 14 publications

Choline Acetyltransferase, Glutamate Decarboxylase, Tyrosine Hydroxylase, Calcitonin Gene‐related Peptide and Opioid Peptides Coexist in Lateral Efferent Neurons of Rat and Guinea‐pig

Choline Acetyltransferase, Glutamate Decarboxylase, Tyrosine Hydroxylase, Calcitonin Gene‐related Peptide and Opioid Peptides Coexist in Lateral Efferent Neurons of Rat and Guinea‐pig

Galanin Gene Expression in Chromaffin Cells Is Controlled by Calcium and Protein Kinase Signaling Pathways

From outer to inner space: Traveling along a scientific career from astro-chemistry to drug research

Contact Info

Product

Resources

About