Monoclonal antibodies against Kell glycoprotein: serology, immunochemistry and quantification of antigen sites

Parsons, Stephen; Gardner, Brigitte; Anstee, D. J.

doi:10.1111/j.1365-3148.1993.tb00051.x

Cited by 36 publications

(31 citation statements)

References 22 publications

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“…BRIO 8 (IgG2a) and BRIC68 (IgG2a) have not been shown to recognize a polymorphism, BRIC107 (IgGl) is anti-klike and BRIC203 (IgGl) is anti-Kpbc [8]. These monoclonal antibod ies were kindly provided by Dr. S. Parsons, IBGRL, Bristol, UK, as culture supernatants.…”

Section: Methodsmentioning

confidence: 99%

“…Using several monoclonal antibodies to high-incidence epitopes on the Kell protein, Parsons et al [8] have shown that BRIC107 (anti-k-like) identifies an epitope that is dis tinct from a set of overlapping epitopes recognized by BRIC18, BRIC68, BRIC203 (anti-Kpbc). These monoclonal antibodies have been used previously by the MAIEA assay to show the relative location of some Kell antigens: K/k (KEL1/KEL2), K22 (KEL22), UP (KEL10), Jsa/Jsb (KEL6/ KEL7), K14 (KEL14), Kpc (KEL21,), K18 (KEL18), Kpa…”

Section: Introductionmentioning

confidence: 99%

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A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

Jones¹,

Reid²,

Oyen³

et al. 1995

Vox Sanguinis

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A 47-year-old native American (TOU) was admitted to hospital for hip surgery. His serum agglutinated all red blood cells (RBCs) tested except Ko and DTT- treated RBCs and was weakly reactive with RBCs known to have a weak expression of Kell antigens, namely Kmod, McLeod, Kp(a+b-) (KEL:3,-4) and K:-13 (KEL:-13) phenotypes. RBCs from three siblings, a son and a daughter were incompatible with TOU’s antibody. TOU’s RBCs had the common Kell phenotype: K- k+ Kp(a-b+c-) Ku+ Js(a-b+) Ul(a-) K:ll, -17 K:14,-24 K: 12,13,18,19, 22,-23 (KEL:-1,2,-3,4,5,-6,7,-10,11,12,13,14,-17,18,19,-21,22,-23,-24). Since TOU’s RBCs were not agglutinated by an unidentified Kell-related antibody (IAN), tests were performed to show that TOU and IAN were mutually compatible. IAN is a Latino female hospitalised for a hysterectomy. The TOU antigen was shown to be located on the Kell glycoprotein by a monoclonal antibody immobilisation of erythrocyte antigen (MAIEA) assay. The unique pattem of reactivity obtained with TOU and IAN antibodies using this assay indicated the TOU epitope to be in an area remote from other Kell antigens, namely K, k, Kp^a, Kp^b, Kp^c, Ku, Js^a, Js^b Ul^a, K11, K12, K13, K14, Wk\ K18, K19, K22 and K24 (KEL1, KEL2, KEL3, KEL4, KEL5, KEL6, KEL7, KEL11, KEL12, KEL13, KEL14, KEL17, KEL18, KEL19, KEL21, KEL22 and KEL24) but close to the low-incidence antigen K23 (KEL23). Investigation of antibodies to previously identified antigens on the Kell glycoprotein by MAIEA using the mouse monoclonal antibodies BRIC18, BRIC68, BRIC107 and BRIC203 has identified six patterns of reactivity and has provided evidence for Kp^c being located in the same region as Kp^a and Kp^b.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

Jones¹,

Reid²,

Oyen³

et al. 1995

Vox Sanguinis

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“…One possibility is that the protein homologues of these antigens are also found in mice and are therefore not recognized by the murine immune system. Alternatively, it may be that there is an overwhelming immune response to the unwanted, but highly immunogenic, antigens such as A, B and H that are present on RBCs, and therefore the antigens of interest escape detection by the murine immune system (Anstee & Edwards, 1982;Parsons, 1985). Table II Kell protein has been successfully expressed on the surface of cultured cells (Lee, 1997;Russo et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…However, monoclonal antibodies (Mabs) are homogenous immunoglobulins secreted by a single clone of immune B cells. Thus, their consistent quality, absence of contaminating antibodies, and unlimited supply make Mabs especially attractive as diagnostic reagents (Parsons et al, 1993). Mabs are also useful research tools as probes for epitope mapping and in the study of the structure and function of proteins that carry blood group antigens (Russo et al, 1998;Anstee et al, 1983;Parsons et al, 1982).…”

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confidence: 99%

Production and characterization of anti‐Kell monoclonal antibodies using transfected cells as the immunogen

et al. 1999

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Summary. Monoclonal antibodies (Mabs) to blood group antigens are valuable as diagnostic reagents for typing red blood cells (RBCs) in the clinical setting, and for structure± function studies of proteins. Here, we report a powerful system that enabled us to produce Mabs to blood group antigens. A murine erythroleukaemia (MEL) cell line expressing Kell protein, a transmembrane glycoprotein that carries a number of clinically relevant antigens, was used as a novel immunogen.

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“…Parsons et al [14] provide evidence that the BRIC 107 epitope is remote from the overlapping sites of BRIC18, BRIC 68 and BRIC 203 ( fig. 5).…”

Section: Discussionmentioning

confidence: 99%

K12 Is Located on the Kell Blood Group Protein in Proximity to K/k and Js^a/Js^b

Reid¹,

Øyen²,

Redman³

et al. 1995

Vox Sanguinis

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K12 is a high-prevalence antigen, with no known antithetical partner, that is associated with the Kell blood group system. We report studies on the 5th propositus (MS) with the K:-12 phenotype and the second case to show that the K12 is inherited. The anti-K12 in his serum did not destroy antigen-positive incompatible red cells transfused on at least 3 occasions over 6 years. Red cell membranes from MS possessed the Kell protein that was indistinguishable from control membranes. K12 antigen was shown by immunoprécipitation to be on a protein with an apparent molecular mass of 93,000 and by monoclonal antibody immobilization of erythrocyte antigens (MAIEA) assay to be on the Kell protein in proximity to K/k and Js^a/Js^b antigens. These data remove the K:-12 phenotype from its current Kell-related (or para Kell) status and elevates the K12 antigen to a bona fide member of the Kell blood group system.

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Monoclonal antibodies against Kell glycoprotein: serology, immunochemistry and quantification of antigen sites

Cited by 36 publications

References 22 publications

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

Production and characterization of anti‐Kell monoclonal antibodies using transfected cells as the immunogen

K12 Is Located on the Kell Blood Group Protein in Proximity to K/k and Js^a/Js^b

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