Monoclonal antibodies against human α-fetoprotein exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

Stefanová, I; Hořejšı́, Václav; Kristofová, H; Angelisová, Pavla; Žižkovský, Václav; Hilgert, I

doi:10.1016/0022-1759(88)90060-9

Cited by 23 publications

(6 citation statements)

References 15 publications

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“…AFP, which may be an early indication of teratoblastoma, hepatocellular carcinoma, and severe congenital fetal defects, is one of the most widely used clinical tumor markers. [25][26][27] In some cases, the concentration of AFP is related to stage of disease. Thus, it is necessary to detect AFP for clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

et al. 2013

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A novel electrochemical immunosensor for the ultrasensitive detection of a-fetoprotein (AFP) is described with the use of a gold nanoparticle/(3-mercaptopropyl) trimethoxysilane modified electrode as a desirable platform and gold nanoparticle functionalized multi-walled carbon nanotube (MWCNT) nanocomposite labeled secondary antibodies (Ab 2 ) as a signal tag. At first, gold nanoparticles (AuNPs) were attached on the MWCNTs surface using poly(diallyldimethylammoniumchloride) (PDDA) as a linkage reagent to obtain AuNP-MWCNT nanocomposites. Subsequently, horseradish peroxidase (HRP) and thionine (Thi) labeled secondary antibodies (Thi-Ab 2 ) were used to bind AuNP-MWCNT nanocomposites with a high load amount and good biological activity. Moreover, the immunosensor can provide amplified signals because of the increased surface area and biocompatibility of AuNP-MWCNT nanocomposites. On the other hand, the AuNP functionalized (3-mercaptopropyl) trimethoxysilanes (MPTS) were used for the biosensor platform to increase the surface area as well as improving the electronic transmission rate to capture a large amount of primary antibodies (Ab 1 ). Thus, amplified signals can be obtained by an electrochemical sandwich immunoassay protocol in the presence of H 2 O 2 . The proposed sensing strategy provides a wide linear dynamic range from 0.01 to 50 ng mL À1 with a low detection limit of 3 pg mL À1 (S/N ¼ 3). Significantly, the developed immunoassay method showed a good stability, selectivity and reproducibility.

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Section: Introductionmentioning

confidence: 99%

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

et al. 2013

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“…The serum concentration of AFP is relatively high in the embryonic period, but that decreases rapidly after birth and falls to normal adult levels by the first year of life. Also, pregnant women who have high levels of AFP may indicate infants with an abnormal fetal brain or spinal cord [2,5]. The serum concentration of AFP in healthy adults is typically less than 25 ng/mL, if the serum levels of AFP increasing, it may predict high risk at HCC, germ cell tumors, metastatic cancer [6,7].…”

Section: Introductionmentioning

confidence: 98%

A Reagentless Amperometric Immunosensor for Alpha‐Fetoprotein Based on Gold Nanoparticles/TiO₂ Colloids/Prussian Blue Modified Platinum Electrode

Yuan

Chai

et al. 2007

Electroanalysis

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A novel reagentless amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was prepared by immobilizing TiO 2 colloids on Prussian blue (PB) modified platinum electrode, which yielded a positively charged interface with strong adsorption to deposit gold nanoparticles for immobilization of alpha-fetoprotein antibody (anti-AFP). The factors influencing the performance of the proposed immunosensors were studied in detail. Under the optimized conditions, cyclic voltammograms determination of AFP showed a specific response in two concentration ranges from 3.0 to 30.0 ng/mL and from 30.0 to 300.0 ng/mL with a detection limit of 1.0 ng/mL at a signal-to-noise ratio of 3. The proposed immunosensor exhibited high selectivity, good reproducibility, long-term stability (> 2 months) and good repeatability.

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“…It is known that the average concentration of AFP is about 25 ng ml À1 in healthy human serum [1] and elevated AFP concentration in adult plasma may be an early indication of hepatocellular carcinoma and teratoblastoma [2]. Thus, it is necessary to measure AFP for the clinical diagnosis and even early detection of original liver carcinoma.…”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer self-assembled multilayer films of multi-walled carbon nanotubes and platinum–Prussian blue hybrid nanoparticles for the fabrication of amperometric immunosensor

Chen

Yuan

et al. 2008

Journal of Electroanalytical Chemistry

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Monoclonal antibodies against human α-fetoprotein exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

Cited by 23 publications

References 15 publications

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

A Reagentless Amperometric Immunosensor for Alpha‐Fetoprotein Based on Gold Nanoparticles/TiO₂ Colloids/Prussian Blue Modified Platinum Electrode

Layer-by-layer self-assembled multilayer films of multi-walled carbon nanotubes and platinum–Prussian blue hybrid nanoparticles for the fabrication of amperometric immunosensor

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Monoclonal antibodies against human α-fetoprotein exploitation of an unusual calcium-dependent interaction with the antigen for analytical and preparative purposes

Cited by 23 publications

References 15 publications

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

Ultrasensitive electrochemical immunosensors for clinical immunoassay using gold nanoparticle coated multi-walled carbon nanotubes as labels and horseradish peroxidase as an enhancer

A Reagentless Amperometric Immunosensor for Alpha‐Fetoprotein Based on Gold Nanoparticles/TiO2 Colloids/Prussian Blue Modified Platinum Electrode

Layer-by-layer self-assembled multilayer films of multi-walled carbon nanotubes and platinum–Prussian blue hybrid nanoparticles for the fabrication of amperometric immunosensor

Contact Info

Product

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A Reagentless Amperometric Immunosensor for Alpha‐Fetoprotein Based on Gold Nanoparticles/TiO₂ Colloids/Prussian Blue Modified Platinum Electrode