Monoclonal antibodies against human T cell leukemia-lymphoma virus (HTLV) p24 internal core protein. Use as diagnostic probes and cellular localization of HTLV.

Abstract

Four monoclonal antibodies, human T cell leukemia-lymphoma virus (HTLV) 6, 7, 8, and 9, which react with the 24,000 dalton internal core protein of HTLVI, have been developed. These monoclonal antibodies reacted with only HTLV-infected cells and not with a broad spectrum of normal, neoplastic, mitogen-stimulated, or virus-infected cells and tissues. HTLV 6, 7, 8, and 9 identified at least two different antigenic determinants on HTLV p24 that were also recognized by antibodies present in HTLV+ patient sera. Monoclonal antibodies HTLV 6, 7, 8, and 9 reacted in indirect immunofluorescence assays with HTLV p24 localized at the cell surface of 5-d cultures of HTLV-infected T cells and, as well, reacted with T cells infected with HTLVII, a new type of HTLV isolated from a patient (MO) with a T cell variant of hairy cell leukemia. Thus, HTLV 6, 7, 8, and 9 should prove to be useful diagnostic reagents in the identification of HTLV- and HTLVII-infected T cells.

In Vitro and In Vivo Characterization of Anthrax Anti-Protective Antigen and Anti-Lethal Factor Monoclonal Antibodies after Passive Transfer in a Mouse Lethal Toxin Challenge Model To Define Correlates of Immunity

“…Female BALB/c mice were similarly immunized with recombinant LF (rLF; List Biological Laboratories, Campbell, CA), and spleens were harvested and used to make hybridomas. Immune spleen cells were fused with mouse myeloma cells using standard procedures routinely used in our laboratory (14,31,35,44,45,52,55). Hybridomas producing anti-rPA or anti-rLF antibodies were screened by enzyme-linked immunosorbent assay (ELISA) using rPA or rLF as the coating antigen, respectively.…”

Viral Aetiology of Adult T-Cell Leukaemia

“…Anti-HTLV p19 was shown to react not only with HTLV-infected lymphocytes but also with the thymus tissue of seronegative humans and syncytial trophoblast cells from normal human placenta (RobertGuroff et al, 1981). Monoclonal antibodies against p24 (Palker et al, 1984;Fujii et al, 1984) and gp21 (Sugamura et al, 1984a) have also been reported. Anti-p24 antibody has been used to identify a precursor protein, p53 (Fujii et al,t984).…”

Epidemiologic assessment of screening tests for antibody to human T lymphotropic virus type I (HTLV-I).

“…13,14 Definitions In this study, a seropositive serum sample was defined by the presence of antibodies to gag (p24) and env (gp46 or precursor gp61/68) on WIB or RIPA. 'o A serum sample with immunoreactivity to at least one HTLV-I gene product but not satisfying these criteria was called indeterminate.…”