Monoclonal antibodies against human astrocytomas and their reactivity pattern

Stavrou, D; Keiditsch, E; Schmidberger, F.; Bise, K.; Funke, I.; Eisenmenger, Wolfgang; Kurrle, R.; Martin, B.; Stöcker, Ulrich

doi:10.1016/0022-510x(87)90155-9

Cited by 28 publications

(9 citation statements)

References 19 publications

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“…GAA-positive/GFAP-positive cells can be of either neoplastic or reactive origin, because GAA positive material could have been delivered from necrotic tumor cells and then have been incorporated by reactive cells. The recognition of reactive astrocytes by glioma-directed MAbs has been described (Stavrou et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

“…Using human astrocytoma cells as immunogen, we have established hybridoma clones producing antibodies, which react predominantly with GAA (Stavrou et al, 1987). In the present study, GFAP and 2 MAb-defined GAA are demonstrated simultaneously in human astrocytomas in order to identify cells which were recognized by anti-glioma MAbs .…”

mentioning

confidence: 88%

“…MUC 2-63 (IgG1) binds to cell surface membranes (native and fixed material) and may also be employed in vivo with good results in external scintigraphy (data not shown). Details on the reactivity patterns have been published (Stavrou et al, 1987).…”

Section: Antibodiesmentioning

confidence: 99%

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Simultaneous Demonstration of glia- and glioma-associated antigens in human astrocytomas

et al. 1988

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Glial flbrillary acidic protein (GFAP) and glioma‐assodated antigens (GAA) defined by monoclonal antibodies (MAbs) were demonstrated simultaneously in human astrocytoma tissue. GFAP was stained by PAP‐method, GAA were visualized by avidin‐biotin‐technique using alcaline phosphatase. In primary and secondary tumors as well as in tissue culture heterogeneity of GFAP‐ and GAA‐expression is obvious. GFAP is mostly restricted to cell processes and less marked in the perinudear space. Depending on the individual antibody, MAbs‐positive material is located either in the tumor cell plasma (MUC 8‐22) or on cell surface membranes (MUC 2‐63). There is remarkable expression of GAA in celt clusters which fail to express Gf AP. At higher magnification, 3 types of cellular reactivity are detectable: (a) cells which react only with anti‐GFAP, (b) cells which react only with anti‐GAA and (c) tells which express both, GFAP and GAA, especially those of protoplasmic astrocyte type. These celts also occur in subcutaneous tumor grafts, and may thus represent not only a reactive event, but be part of tumor cell populations.

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Section: Discussionmentioning

confidence: 99%

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confidence: 88%

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Simultaneous Demonstration of glia- and glioma-associated antigens in human astrocytomas

et al. 1988

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“…23 Infection with HIV-I was achieved by cocultivation of 85HG-66 cells with T-lymphoma cells (KE37/I) productively infected with HIV-illB. Expression ofHIV-1 antigen following exposure of cells to virus was monitored at a single-cell level by direct immunoperoxidase staining (IPS).…”

Section: Establishment Of Chronically Hiv-i-infected Human Glioma Celmentioning

confidence: 99%

Molecular Interaction of HIV-1 in Glioma Cells

Erfle

Kleinschmidt

Neumann

et al. 1995

Technical Advances in AIDS Research in the Human Nervous System

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“…Accordingly, SGCs have high potential to express symbiotic markers. Many types of whole cells have been used as antigens to immunize mice and generate antibodies that could recognize cell-surface antigens, such as human astrocytomas 19 , human B lymphoblastic leukemia cells 20 , Burkitt’s lymphoma tumor cells 21 , sheep red blood cells 22 , and killed whole cells of Streptococcus mutans 23 . Therefore, in the present study, whole SGCs were used as the antigen to generate symbiosis-specific antibodies for possible symbiotic markers to investigate the characteristics of cnidarian-dinoflagellate endosymbiosis.…”

Section: Introductionmentioning

confidence: 99%

Generation of clade- and symbiont-specific antibodies to characterize marker molecules during Cnidaria-Symbiodinium endosymbiosis

Huang

Lin

et al. 2017

Sci Rep

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The endosymbiosis between cnidarians and dinoflagellates is responsible for the formation of coral reefs. Changes in molecules have been identified during the process of cnidaria-Symbiodinium endosymbiosis. However, the complexity of the molecular interaction has prevented the establishment of a mechanistic explanation of cellular regulation in this mutualistic symbiosis. To date, no marker molecules have been identified to specifically represent the symbiotic status. Because the endosymbiotic association occurs in the symbiotic gastrodermal cells (SGCs), whole cells of isolated SGCs were used as an antigen to generate monoclonal antibodies (mAb) to screen possible molecular candidates of symbiotic markers. The results showed that one of the generated monoclonal antibodies, 2–6F, specifically recognized clade C symbiotic Symbiodinium but not its free-living counterpart or other Symbiodinium clades. The expression levels of 2–6F mAb-recognized proteins are highly correlated with the symbiotic status, and these proteins were characterized as N-linked glycoproteins via treatment with peptide N-glycosidase F. Furthermore, their glycan moieties were markedly different from those of free-living Symbiodinium, potentially suggesting host regulation of post-translational modification. Consequently, the 2–6F mAb can be used to detect the symbiotic state of corals and investigate the complex molecular interactions in cnidaria-Symbiodinium endosymbiosis.

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Monoclonal antibodies against human astrocytomas and their reactivity pattern

Cited by 28 publications

References 19 publications

Simultaneous Demonstration of glia- and glioma-associated antigens in human astrocytomas

Simultaneous Demonstration of glia- and glioma-associated antigens in human astrocytomas

Molecular Interaction of HIV-1 in Glioma Cells

Generation of clade- and symbiont-specific antibodies to characterize marker molecules during Cnidaria-Symbiodinium endosymbiosis

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