Monoclonal antibodies against Caprine arthritis-encephalitis virus epitopes in the p28 and p55gag viral proteins

Brandão, Camila Fonseca Lopes; Campos, Gúbio Soares; Silva, Ana Carolina Requião; Torres, Jorge Martínez; Tigre, Dellane Martins; Sardi, Silvia Inês

doi:10.1016/j.jviromet.2012.10.020

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…The IDEXX ELISA kit, which was used in our study, uses a mixture of the transmembrane protein (TM ENV gene) and recombinant p28 protein (GaG gene) for the detection of the infection as a multi-antigen approach. It was reported that monoclonal antibodies against p28 are also reactive against p55 (gag) protein and the intermediate cleavage products, p44, p36, and p22 [ 38 ]. The antibody response was significantly higher among arthritic than asymptomatic goats [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

et al. 2021

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Background and Aim: Several reports described the detection of specific caprine arthritis-encephalitis virus (CAEV) antibodies in Russian goat populations, which indicates the circulation of CAEV in Russian goat farms. The aim of this study was to use a multi-target approach to testing with both serological tests and an in-house real-time (RT) molecular test to investigate the prevalence of CAEV in goats from three hobbyist farms in the Republic of Tatarstan, Russia. Materials and Methods: We applied a multi-target approach to testing with both enzyme-linked immunosorbent assay (ELISA) and an in-house RT polymerase chain reaction test to investigate the prevalence of CAEV in goats. Animals from the three hobbyist farms were used in this study. The animals from two farms (n=13 for F1 and n=8 for F2) had clinical signs of arthritis and mastitis. In the third farm (n=15 for F3), all goats were home-bred and had no contact with imported animals. Results: CAEV antibodies (ELISA targets TM env and gag genes) were detected in serum samples from two farms (F1 and F2), indicating seroprevalence of 87.50-92.31%. Specific CAEV antibodies were also detected in milk samples. CAEV proviral DNA was detected in 53.85-62.50%. The results from all tests performed in the third farm (F3) were negative, indicating that all tests were 100% specific. Conclusion: The results showed that CAEV is circulating and present in small hobbyist goat farms in Russia. Serological and molecular tests could be important for programs to control and eradicate CAEV in Russia for hobbyist goat farms.

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Section: Discussionmentioning

confidence: 99%

Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

et al. 2021

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“…The IDEXX ELISA kit, which was used in our study, uses a mixture of the transmembrane protein (TM ENV gene) and recombinant p28 protein (GaG gene) for detection of the infection as a multi-antigen approach. It was reported that monoclonal antibodies against p28 are also reactive against p55 (gag) protein and the intermediate cleavage products, p44, p36 and p22 [38]. The antibody response was significantly higher among arthritic than asymptomatic goats [28].…”

Section: Discussionmentioning

confidence: 99%

Initial Multitarget Approach Shows Importance for Improved Caprine Arthritis-encephalitis Virus Control Program in Russia for Hobbyist Goat Farms

Shuralev¹,

Khammadov²,

Osyanin³

et al. 2021

Preprint

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Aim: The aim of this study was to use a multi target approach to testing with both serological tests and an in-house real-time molecular test to investigate the prevalence of the caprine arthritis-encephalitis virus (CAEV) in goats from three hobbyist farms in the Republic of Tatarstan, Russia. Materials and Methods: We have approached the detection of using a multi target approach testing with both ELISA and an in-house real-time PCR test to investigate the prevalence of CAEV in goats. Animals from three hobbyist farms were used in this study. The animals from two farms (n=13 for F1 and n=8 for F2) had clinical signs of arthritis and mastitis. In the third farm (n=15 for F3), all goats were homebred and had no contact with imported animals. Results: CAEV antibodies (ELISA targets TM env and gag genes) were detected in serum samples from two farms (F1 and F2), indicating a seroprevalence 87.50-92.31%. Specific CAEV antibodies were also detected in milk samples. CAEV proviral DNA was detected in 53.85-62.50%. Results from all tests performed in the third farm (F3) were negative, indicting all tests were 100% specific.Conclusion: Results of this work show that CAEV is circulating and present in small hobbyist goat farms in Russia. Serological and molecular tests could be of importance for CAEV control and eradication programs in Russia for hobbyist goat farms.

show abstract

Section: Discussionmentioning

confidence: 99%

Initial Multitarget Approach Shows Importance for Improved Caprine Arthritis-encephalitis Virus Control Program in Russia for Hobbyist Goat Farms

Shuralev¹,

Khammadov²,

Osyanin³

et al. 2020

Preprint

View full text Add to dashboard Cite

In this study we have approached the detection of caprine arthritis-encephalitis virus (CAEV) using a multi target approach testing with both ELISA and an in-house real-time PCR test to investigate the prevalence of CAEV in goats from hobbyist farms in the Republic of Tatarstan, Russia. Animals from three hobbyist farms were used in this study. The animals from two farms (n=13 for F1 and n=8 for F2) had clinical signs of arthritis and mastitis. In the third farm (n=15 for F3), all goats were homebred and had no contact with imported animals. CAEV antibodies (ELISA targets TM ENV and GaG genes) were detected in serum samples from two farms (F1 and F2), indicating a seroprevalence 87.50-92.31%. Specific CAEV antibodies were also detected in milk samples. CAEV proviral DNA was detected in 53.85-62.50%. Results from all tests performed in the third farm (F3) were negative, indicting all tests apparent specificity of 100%. The results of this work show that CAEV is circulating and present in small hobbyist goat farms in Russia. Serological and molecular tests could be of importance for CAEV control and eradication programs in Russia for hobbyist goat farms.

show abstract

Monoclonal antibodies against Caprine arthritis-encephalitis virus epitopes in the p28 and p55gag viral proteins

Cited by 6 publications

References 14 publications

Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

Initial multi-target approach shows importance of improved caprine arthritis-encephalitis virus control program in Russia for hobbyist goat farms

Initial Multitarget Approach Shows Importance for Improved Caprine Arthritis-encephalitis Virus Control Program in Russia for Hobbyist Goat Farms

Initial Multitarget Approach Shows Importance for Improved Caprine Arthritis-encephalitis Virus Control Program in Russia for Hobbyist Goat Farms

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