Monoclonal antibodies against a Marek's disease tumor-associated surface antigen.

Higashihara, Tomoko; Mikami, Takeshi; Ohashi, Kazuhiko; Kodama, Hiroshi; Onuma, Misao; Izawa, Hisao

doi:10.1292/jvms1939.46.649

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“…The specificity of 2B9 to MATSA is confirmed by the absorption test using MD lymphoma (Higashihara et al, 19846) and the blocking CELISA test using anti-MATSA serum (Horie er al., 1989). There is no cross-reactivity of 2B9 with the antigen expressed on normal chicken lymphocytes and lymphoblastoid cells derived from avian leukosis (AL) or reticuloendotheliosis (RE) by the indirect IF tests (Hone et al., 1989;Higashihara et al, 1984b). We used 2B9 antibody and highly-absorbed anti-MATSA rabbit serum, whose specificity against MATSA was demonstrated (Horie et al, 1989), as tools of physico-chemical characterization and purification of MATSA on the surface of MSB1-clo.18 cells.…”

Section: Discussionmentioning

confidence: 99%

Analysis of marek's disease tumor‐associated surface antigen on MDCC‐MSB1‐CLO.18 cells

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Kodama

et al. 1991

Intl Journal of Cancer

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The physico-chemical nature of Marek's disease tumor-associated surface antigen (MATSA) on Marek's disease (MD) lymphoblastoid tumor cell line (MDCC-MSB1-clo.18) was examined by the cellular enzyme-linked immunosorbent assay (CELISA) and the sandwich enzyme-linked immunosorbent assay (ELISA) using an anti-MATSA immune serum or a monoclonal antibody (MAb) 2B9 developed against MATSA. Our results indicate that MATSA is a glycoprotein and 2B9 recognizes an antigenic site in the protein moiety of MATSA. MATSA was solubilized from MSB1-clo.18 cells by treatment with 0.5% Nonidet P-40, and purified by affinity chromatography coupling with 2B9 and further by ion exchange chromatography on diethylaminoethylcellulose (IECD). MATSA was eluted with 0.2 to 0.3 M KCl in IECD and the purity of MATSA was increased about 2,500-fold. The purified MATSA was shown to have a molecular weight (Mr) of 70,000 by SDS-PAGE. The reactivity of purified MATSA with anti-thymus cell serum was examined. MATSA was detectable by anti-thymus cell serum, although 2B9, which was used to purify MATSA from MSB1-clo.18 cells, was not reactive to cells prepared from the thymus. However, MATSA was no longer detectable after the absorption of anti-thymus cell serum by chicken bursa cells. The absorption of anti-thymus cell serum by chicken red blood cells (RBC) had no effect on the reactivity against MATSA. These results suggest that MATSA may be a lymphocyte-specific antigen modified during leukemogenesis by Marek's disease virus (MDV).

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Section: Discussionmentioning

confidence: 99%