Monoamines of taste buds in the fungiform and foliate papillae of the mouse.

Takeda, Masako; Shishido, Yoko; Kitao, Kenji

doi:10.1679/aohc.45.239

Cited by 22 publications

(7 citation statements)

References 10 publications

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“…Serotonin was also reported to occur in type I11 taste cells in rabbit and monkey taste buds (Fujimoto et al, 1987). However, in taste buds from mice, serotonin was detected only after administering its precursor, 5-hydroxytryptophan (5-HTP; Geerdink and Drukker, 1973;Takeda and Kitao, 1980;Takeda et al, 1982;Suzuki and Takeda, 1983). Because 5-HTP uptake is not a reliable and unique marker for serotonergic cells, the presence of this monoamine in untreated mammalian taste buds was not established by these studies.…”

Section: Indexing Terms: Chemosensory Cells Gustation Monoamines Imentioning

confidence: 99%

Localization of serotonin in taste buds: A comparative study in four vertebrates

Kim

Roper

1995

J of Comparative Neurology

101

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To investigate monoaminergic synaptic mechanisms in taste buds, we examined taste buds of mice, rats, rabbits, and mudpuppies for the presence of the neurotransmitter candidate, serotonin. Immunocytochemistry revealed serotonin-like immunostaining in cells in mammalian taste buds and Merkel-like basal cells in taste buds of mudpuppies. In untreated mudpuppies and in mammals injected with the precursor to serotonin, L-tryptophan, certain taste cells showed serotonin-like immunoreactivity, although in mammalian taste buds the immunostaining was relatively weak. After pretreating mammals with 5-hydroxytryptophan (5-HTP), the intermediate precursor between L-tryptophan and serotonin, several taste cells showed strong immunoreactivity for serotonin. These findings indicate that mammalian taste cells normally contain serotonin and that taste cells can take up 5-HTP and convert it to serotonin. Immunocytochemistry on wholemount preparations demonstrated that serotonergic cells of mudpuppies (i.e., Merkel-like basal cells) were disposed in a ring at the periphery of taste buds. Similarly, serotonergic cells in mammalian taste buds tended to be located at the periphery of taste buds. Based on the position of serotonergic cells in the taste bud and on recent physiological studies on the actions of serotonin in taste buds, we postulate that serotonin functions as a neuromodulator or neurotransmitter in vertebrate taste buds.

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Section: Indexing Terms: Chemosensory Cells Gustation Monoamines Imentioning

confidence: 99%

Localization of serotonin in taste buds: A comparative study in four vertebrates

Kim

Roper

1995

J of Comparative Neurology

101

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“…One group of investigators has classified taste cells into dark, intermediate, and light cell types and suggested that the different cellular morphologies represent stages in development of a single cell line within taste buds Delay et al, 1986;Royer and Kinnamon, 1988). Other investigators have taken the view that three distinct cell types (types I, 11, and 111) are present in the taste buds of mice, analogous to those in the rabbit (Takeda and Hoshino, 1975;Takeda, 1976Takeda, ,1977Takeda and Kitao, 1980;Takeda et al, 1982Takeda et al, , 1985Murray, 1993). Both groups have agreed on the presence of undifferentiated peripheral cells and basal cells (type IV).…”

Section: Introductionmentioning

confidence: 99%

HVEM ultrastructural analysis of mouse fungiform taste buds, cell types, and associated synapses

Kinnamon

Henzler

Royer

1993

Microscopy Res & Technique

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We have used high voltage electron microscopy and computer-generated three-dimensional reconstructions from serial sections to elucidate the structure of taste bud cells and their associated synapses in fungiform taste buds of the mouse. Five fungiform taste buds (two of which were serially sectioned) were examined with the high-voltage electron microscope (HVEM). We identified the synaptic connections from taste cells onto sensory nerve fibers and classified the presynaptic taste cells based on previously established ultrastructural criteria. From those data we have distinguished dark, intermediate, and light cells in murine fungiform taste buds. Synapses in murine fungiform taste buds are fewer in number, but contain many more vesicles than synapses in either foliate or circumvallate taste buds. Synapses in mouse circumvallate and foliate taste buds typically contain a few to several synaptic vesicles per section, whereas fungiform synapses may have in excess of 100 vesicles per profile. The significance of these differences in the numbers of synapses and synaptic structure between fungiform and circumvallate/foliate synapses is not known. Based on the small number of synapses observed in fungiform taste buds, we speculate that fungiform taste buds have only a few cells transducing sensory stimuli at any given time. Alternatively, communication of sensory information from the taste receptor cells to the afferent nerve fibers may be mediated by some other mechanism(s) in addition to classical chemical synapses.

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“…Verification of the existence of a type I11 cell is essential to the proof of three separate cell lines in the mouse. The independent existence of this cell type in the mouse has been supported in a series of reports (Nada and Hirata, 1975;Takeda, 1976Takeda, , 1977Takeda and Kitao, 1980;Takeda et al, 1982Takeda et al, , 1985. Identification of the type I11 cell in the rabbit foliate buds rests on the presence of small dense-cored vesicles, "chemical" synaptic contact and a prominent apical process that extends well out through the pore (Murray, 1986).…”

Section: Analysis and Discussionmentioning

confidence: 84%

Cellular relations in mouse circumvallate taste buds

Murray

1993

Microscopy Res & Technique

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The fine structure of the taste buds of circumvallate papillae of two strains of mice was studied by electron microscopy. Mice anesthetized with ketamine were perfused through the heart with a double aldehyde mixture in cacodylate buffer and the tissues embedded in Epon. Semi-serial sections were employed. The morphology and relationships of cell types are consistent with the majority of descriptions of mammalian taste buds served by the ninth cranial nerve. Cells of type II are particularly well documented, as the stages in their origin, maturation and degeneration could be followed. Significant differences, however, relate to cell type I. These cells contain large dense-cored granules, contrasted with the more irregular and somewhat larger dark granules of the type I cells in the rabbit. These granules do not produce a dense homogenous product for the pore, as seen in the rabbit. Rather the pore substance consists of small, empty vesicles in a diffuse dark matrix. These granules are only moderately larger than the dense-cored vesicles of the type III cells. All features of the type III cell were demonstrated, although no instance of a complete cell was seen in any section. No significant differences were noted between the two strains of mice. Intimate proximity of a nerve to a cell nucleolus, suggestive of a trophic pathway, is illustrated.

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Monoamines of taste buds in the fungiform and foliate papillae of the mouse.

Cited by 22 publications

References 10 publications

Localization of serotonin in taste buds: A comparative study in four vertebrates

Localization of serotonin in taste buds: A comparative study in four vertebrates

HVEM ultrastructural analysis of mouse fungiform taste buds, cell types, and associated synapses

Cellular relations in mouse circumvallate taste buds

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