Monoamine oxidase activities in human cystic and colonic arteries — influence of age

Figueiredo, Isabel Vitória; Coscolla, A. Martinez; Cotrim, Maria Dulce; Caramona, Margarida; Callingham, B. A.

doi:10.1007/978-3-7091-6499-0_20

Cited by 4 publications

(3 citation statements)

References 18 publications

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“…For selective MAO-A and MAO-B inhibition, 0.1 mM selegiline was used as an MAO-B inhibitor and 1.0 µM clorgyline as an MAO-A inhibitor (10). All other reagents used were of analytical grade.…”

Section: Methodsmentioning

confidence: 99%

Correlation between total nitrite/nitrate concentrations and monoamine oxidase (types A and B) and semicarbazide-sensitive amine oxidase enzymatic activities in human mesenteric arteries from non-diabetic and type 2 diabetic patients

Nunes

Figueiredo

Pereira

et al. 2012

Braz J Med Biol Res

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The aim of this study was to determine the correlation between total nitrite/nitrate concentrations (NOx) and the kinetic parameters of monoamine oxidase enzymes (MAO-A and MAO-B) and semicarbazide-sensitive amine oxidase (SSAO) in human mesenteric arteries. Arteries were from non-diabetic and type 2 diabetic patients with sigmoid or rectum carcinoma for whom surgery was the first option and who were not exposed to neo-adjuvant therapy. Segments of human inferior mesenteric arteries from non-diabetic (61.1 ± 8.9 years old, 7 males and 5 females, N = 12) and type 2 diabetic patients (65.8 ± 6.2 years old, 8 males and 4 females, N = 12) were used to determine NOx concentrations and the kinetic parameters of MAO-A, MAO-B and SSAO by the Griess reaction and by radiochemical assay, respectively. The NOx concentrations in arteries from diabetic patients did not differ significantly from those of the non-diabetic group (10.28 ± 4.61 vs 10.71 ± 4.32 nmol/mg protein, respectively). In the non-diabetic group, there was a positive correlation between NOx concentrations and MAO-B parameters: Km (r = 0.612, P = 0.034) and Vmax (r = 0.593, P = 0.042), and a negative correlation with the SSAO parameters: Km (r = -0.625, P = 0.029) and Vmax (r = -0.754, P = 0.005). However, in the diabetic group no correlation was found between NOx concentrations and the three kinetic parameters of the enzymes. These results suggest an important function of sympathetic nerves and vascular NOx concentrations in arteries of non-diabetic patients. Thus, these results confirm the importance of a balance between oxidants and antioxidants in the maintenance of vascular homeostasis to prevent oxidative stress.

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Section: Methodsmentioning

confidence: 99%

Correlation between total nitrite/nitrate concentrations and monoamine oxidase (types A and B) and semicarbazide-sensitive amine oxidase enzymatic activities in human mesenteric arteries from non-diabetic and type 2 diabetic patients

Nunes

Figueiredo

Pereira

et al. 2012

Braz J Med Biol Res

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“…MAO (A and B) and SSAO activities were determined by radiochemical methods using 3 H-5hydroxytryptamine creatinine sulphate ( 3 H-5-HT), in a concentration range between 50-1 000 μM for MAO-A, 14 C-β-phenylethylamine hydrochloride ( 14 C-β-PEA), 5-160 μM for MAO-B and 14 C-benzylamine ( 14 C-BZ), in a concentration range 50-1 600 μM for SSAO (Figueiredo et al 1998). 5-HT, β-PEA and BZ were purchased from Sigma-Aldrich (Madrid, Spain), 3 H-5-HT (15.1 Ci/mmol) and 14 C-BZ (54 mCi/mmol) from Amersham International (London, United Kingdom) and 14 C-β-PEA (43.8 mCi/mmol) from Perkin Elmer LAS (Boston, MA).…”

Section: Determination Of Mao (A and B) And Ssao In The Vascular Tissuesmentioning

confidence: 99%

“…This enzyme is also known as vascular adhesion protein-1 (VAP-1) and plays an important role in the adhesion and migration of leukocytes to sites of inflammation (Bour et al 2009). The mesenteric innervation system plays an important role in controlling mesenteric capacitance, and there are some studies reporting MAO (type A and B) and SSAO activity in non-diabetic patients (Figueiredo et al 1998, Birch et al 2008. However, the impact of those enzymes in the inferior mesenteric arteries of patients with type 2 diabetes remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Monoamine Oxidase and Semicarbazide-Sensitive Amine Oxidase Kinetic Analysis in Mesenteric Arteries of Patients With Type 2 Diabetes

Nunes

Figueiredo²,

Pereira³

et al. 2011

Physiol Res

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Monoamine oxidase (MAO, type A and B) and semicarbazide-sensitive amine oxidase (SSAO) metabolize biogenic amines, however, the impact of these enzymes in arteries from patients with type 2 diabetes remains poorly understood. We investigated the kinetic parameters of the enzymes to establish putative correlations with noradrenaline (NA) content and patient age in human mesenteric arteries from type 2 diabetic patients. The kinetic parameters were evaluated by radiochemical assay and NA content by high-performance liquid chromatography (HPLC). The activity of MAO-A and SSAO in type 2 diabetic vascular tissues was significantly lower compared to the activity obtained in non-diabetic tissues. In the correlation between MAO-A (Km) and NA content, we found a positive correlation for both the diabetic and non-diabetic group, but no correlation was established for patient age. In both groups, MAO-B (Vmax) showed a negative correlation with age. The results show that MAO-A and SSAO activities and NA content of type 2 diabetic tissues are lower compared to the non-diabetic tissues, while MAO-B activity remained unchanged. These remarks suggest that MAO-A and SSAO may play an important role in vascular tissue as well as in the vascular pathophysiology of type 2 diabetes.

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Changes in the activities of semicarbazide-sensitive amine oxidase in inferior mesenteric artery segments and in serum of patients with type 2 diabetes

et al. 2010

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This study aimed to evaluate the semicarbazide-sensitive amine oxidase (SSAO) activity in human arterial tissues and in serum of patients with type 2 diabetes. The SSAO activity, with (14)C-benzylamine as substrate, was measured in homogenates of human inferior mesenteric arteries obtained at surgery, from 10 patients with type 2 diabetes and 16 non-diabetic patients and in the serum of 39 patients with type 2 diabetes and 40 non-diabetic control patients. The SSAO activity in the homogenates of vascular tissue was significantly lower in the diabetics than in the non-diabetics (P = 0.001). The SSAO activity in the serum of patients with type 2 diabetes was higher when compared with control group (P = 0.0001). In conclusion, the SSAO activity increased in the serum and decreased in the arterial tissue. These findings suggest damage in the vascular tissue and support the hypothesis that serum SSAO may be a useful biochemical marker for diabetes.

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Monoamine oxidase activities in human cystic and colonic arteries — influence of age

Cited by 4 publications

References 18 publications

Correlation between total nitrite/nitrate concentrations and monoamine oxidase (types A and B) and semicarbazide-sensitive amine oxidase enzymatic activities in human mesenteric arteries from non-diabetic and type 2 diabetic patients

Correlation between total nitrite/nitrate concentrations and monoamine oxidase (types A and B) and semicarbazide-sensitive amine oxidase enzymatic activities in human mesenteric arteries from non-diabetic and type 2 diabetic patients

Monoamine Oxidase and Semicarbazide-Sensitive Amine Oxidase Kinetic Analysis in Mesenteric Arteries of Patients With Type 2 Diabetes

Changes in the activities of semicarbazide-sensitive amine oxidase in inferior mesenteric artery segments and in serum of patients with type 2 diabetes

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